Expression of pyruvate kinase M2 in preneoplastic hepatic foci of N-nitrosomorpholine-treated rats (original) (raw)

Abstract

The expression of the pyruvate kinase (PK) isoenzymes L and M2 was analysed in the livers of rats treated with the hepatocarcinogenic agent _N_-nitrosomorpholine (NNM) in the drinking water. In control animals L-PK expression was restricted to liver parenchymal cells, whereas M2-PK was detected in bile duct epithelial, blood vessel wall, endothelial and Kupffer cells. In rats treated with NNM proliferating oval cells were consistently L-PK negative and M2-PK positive, while the ductal cells of cholangiofibroses were clearly L-PK positive and coexpressed M2-PK. However, no morphological differentiation of ductal cells into hepatocyte-like cells was observed. In the clear and acidophilic cell foci storing glycogen in excess strong staining for L-PK was observed. In glycogen-poor foci induced by NNM a shift from L-PK to M2-PK expression takes place.

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Authors and Affiliations

  1. Institute of Toxicology, University of Mainz, Mainz, Germany, , , , , , DE
    P. Steinberg, A. Klingelhöffer & F. Oesch
  2. Institute of Toxicology, E. Merck KGaA, Darmstadt, Germany, , , , , , DE
    A. Schäfer, G. Wüst & G. Weiße
  3. Institute of Biochemistry and Endocrinology, University of Giessen, Darmstadt, Germany, , , , , , DE
    E. Eigenbrodt

Authors

  1. P. Steinberg
  2. A. Klingelhöffer
  3. A. Schäfer
  4. G. Wüst
  5. G. Weiße
  6. F. Oesch
  7. E. Eigenbrodt

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Received: 24 March 1998 / Accepted: 13 November 1998

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Steinberg, P., Klingelhöffer, A., Schäfer, A. et al. Expression of pyruvate kinase M2 in preneoplastic hepatic foci of _N_-nitrosomorpholine-treated rats.Virchows Archiv 434, 213–220 (1999). https://doi.org/10.1007/s004280050330

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