Genetic profile of 22 pancreatic carcinoma cell lines (original) (raw)

Abstract.

The K-ras, p53, p16 and DPC4 genes are among those most frequently altered in pancreatic ductal carcinoma. We analyzed 22 cell lines for alterations in these genes by direct sequence analysis and methylation-specific polymerase chain reaction. These cell lines showed mutations in K-ras and p53 at frequencies of 91% and 95%, respectively. Alterations in p16 INK4a were found in all cases and included nine homozygous deletions, seven mutations and promoter methylation in six cases. Eight cell lines (36%) had an alteration of DPC4, including one mutation and seven homozygous deletions. The most typical mutational profile involved K-ras, p53, and p16 INK4a, concurrently aberrated in 20 cases (91%). Eight cell lines had alterations in all four genes. Inactivation of DPC4 was always accompanied by alteration of all of the other three genes. This comprehensive data regarding the cumulative genetic alterations in pancreatic carcinoma cell lines will be of great value for studies involving drug sensitivity or resistance that may be associated with inactivation of a particular gene or molecular pathway.

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Authors and Affiliations

  1. Department of Pathology, University of Verona, Strada Le Grazie, I-37134 Verona, Italy
    Patrick S. Moore, Simonetta Orlandini, Claudio Sorio & Aldo Scarpa
  2. Department of Surgery, University of Verona, Italy
    Claudio Bassi
  3. Department of Internal Medicine I, University of Ulm, Germany
    Thomas Gress
  4. ICRF Molecular Oncology Unit, Department of Cancer Medicine, Imperial College School of Medicine, London, UK
    Nicholas R. Lemoine
  5. Unitat de Biologia Cellular i Molecular, Institut Municipal d'Investigació Mèdica, Barcelona, Spain
    Francisco X. Real
  6. Department of Pathology and Molecular Oncology of Clinic of General Surgery University of Kiel, Germany
    Bence Sipos, Günter Klöppel, Holger Kalthoff & Hendrik Ungefroren
  7. IV Department of Internal Medicine, Medical Faculty of Mannheim, University of Heidelberg, Mannheim, Germany
    Matthias Löhr

Authors

  1. Patrick S. Moore
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  2. Bence Sipos
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  3. Simonetta Orlandini
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  4. Claudio Sorio
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  5. Francisco X. Real
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  6. Nicholas R. Lemoine
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  7. Thomas Gress
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  8. Claudio Bassi
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  9. Günter Klöppel
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  10. Holger Kalthoff
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  11. Hendrik Ungefroren
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  12. Matthias Löhr
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  13. Aldo Scarpa
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Moore, P.S., Sipos, B., Orlandini, S. et al. Genetic profile of 22 pancreatic carcinoma cell lines.Virchows Arch 439, 798–802 (2001). https://doi.org/10.1007/s004280100474

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