Elevated activity and expression of Src-family kinases in human breast carcinoma tissue versus matched non-tumor tissue (original) (raw)

Abstract

Src-family kinase expression was measured in 52 human mammary tumor (T) specimens compared with non-tumor (NT) tissue from the same patient by enzymatic assays employing a Src-kinase family-specific peptide substrate and by immunoblotting with an antibody recognizing the Src-family kinases Src, Fyn, and Yes. In the T specimens, the mean enzymatic activity was moderately elevated (T: 160 fmol ATP min−1 mg−1; NT: 115 fmol ATP min−1 mg−1) with 25 tumor samples having higher activity than the corresponding NT tissue, 17 having lower activity, and no activity detectable in ten T/NT pairs. Immunoblotting revealed clearly elevated expression in 25 tumor tissues and no differences or expression below the detection limit in the remaining T/NT pairs. The data are in agreement with a possible role of Src-family kinases for the biology of mammary carcinoma.

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Authors and Affiliations

  1. Research Unit “Molecular Cell Biology”, Friedrich Schiller University, Drackendorfer Strasse 1, 07747 Jena, Germany Fax: +49-3641-304462 e-mail: i5frbo@rz.uni-jena.de;, , , , , , DE
    Diana Reissig & Frank D. Böhmer
  2. Department of Internal Medicine II, Friedrich Schiller University, Erlanger Allee 101, Jena, Germany, , , , , , DE
    Joachim Clement
  3. Institute of Pathology, Bad Berka, Germany, , , , , , DE
    Jörg Sänger
  4. Institute of Pathology, Friedrich Schiller University, Jena, Germany, , , , , , DE
    Alexander Berndt & Hartwig Kosmehl

Authors

  1. Diana Reissig
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  2. Joachim Clement
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  3. Jörg Sänger
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  4. Alexander Berndt
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  5. Hartwig Kosmehl
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  6. Frank D. Böhmer
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Received: 20 April 2000 / Accepted: 22 August 2000

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Reissig, D., Clement, J., Sänger, J. et al. Elevated activity and expression of Src-family kinases in human breast carcinoma tissue versus matched non-tumor tissue.J Cancer Res Clin Oncol 127, 226–230 (2001). https://doi.org/10.1007/s004320000197

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