Degradation of human secretory immunoglobulin A by Blastocystis (original) (raw)

Abstract

Microbial immunoglobulin A (IgA) proteases cleave human secretory IgA, promoting the mucosal adhesion of pathogens. To investigate if the enteric protozoan Blastocystis degrades human secretory IgA, cell lysate and conditioned medium from two species were exposed to immunoglobulin A. Secretory IgA was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in B. hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo.

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Acknowledgements

This work was supported by generous grants from the National Medical Research Council (R-182-000-058-213) and the Faculty of Medicine, National University of Singapore. We thank Ms. Geok Choo Ng, Mr. Ramachandran for technical support and helpful discussions. Thanks are also due to Ms. Tan Mui Hong, Ms. Tan Li Li and Dr. Punam Dahiya, for their contributions. The present experiments were carried out in compliance with the laws and regulations of Singapore.

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Authors and Affiliations

1. Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore, 117597
   Manoj K. Puthia, Aparna Vaithilingam & Kevin S. W. Tan
2. Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, DSO (Kent Ridge), Singapore, 117510
   Manoj K. Puthia & Jia Lu

Authors

1. Manoj K. Puthia
2. Aparna Vaithilingam
3. Jia Lu
4. Kevin S. W. Tan

Corresponding author

Correspondence toKevin S. W. Tan.

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Puthia, M.K., Vaithilingam, A., Lu, J. et al. Degradation of human secretory immunoglobulin A by Blastocystis.Parasitol Res 97, 386–389 (2005). https://doi.org/10.1007/s00436-005-1461-0

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• Received: 20 June 2005
• Accepted: 27 June 2005
• Published: 07 September 2005
• Issue date: November 2005
• DOI: https://doi.org/10.1007/s00436-005-1461-0

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