Sensitive and species-specific detection of Clonorchis sinensis by PCR in infected snails and fishes (original) (raw)

Abstract

In the present study, PCR procedures have been established for a rapid and easy preparation of DNA of parasite stages from the intermediate hosts, i.e. sporocysts and rediae in snails and metacercariae in fishes. Primers have been developed, which enable a highly sensitive and species-specific detection of Clonorchis sinensis.

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References

• Adams R, Arnold H, Pipitgool V, Sithithaworn P, Hinz E, Storch V (1993) Studies on lophocercariae from Bithynia siamensis goniumphalus (Prosobranchia: Bithyniidae). Southeast Asian J Trop Med Public Health 24:697–700
  Google Scholar
• Haas W, Granzer M, Brockelman CR (1990) Opisthorchis viverrini: finding and recognition of the fish host by the cercariae. Exp Parasitol 71:422–431
  Article PubMed CAS Google Scholar
• Le TH, Van De N, Blair D, Sithithaworn P, McManus DP (2006) Clonorchis sinensis and Opisthorchis viverrini: development of a mitochondrial-based multiplex PCR for their identification and discrimination. Exp Parasitol 112:109–114
  Article PubMed CAS Google Scholar
• Mehlhorn H (ed) (2001) Encyclopedic references of parasitology, vol 1, 2. Springer, Berlin Heidelberg New York
  Google Scholar
• Mehlhorn H, Kojima S, Rim HR, Ruenwongsa P, Andrews P, Thomas H, Bunnag (1983) Ultrastructural investigations on the effects of praziquantel on human trematodes from Asia (Clonorchis sinensis, Metagonimus yokogawai, Opisthorchis viverrini, Paragonimus westermani, Schistosoma japonicum). Drug Res 33:91–98
  CAS Google Scholar
• Müller B, Schmidt J, Mehlhorn H (2006) PCR diagnosis of infections with different species of Opisthorchiidae using a rapid clean up procedure for stool samples and specific primers. Parasitol Res (in press)
• Rim HJ (1986) The current pathology and chemotherapy of clonorchiasis. Korean J Parasitol 24(Suppl, Monographic Series No 3):78–85
  Article Google Scholar
• Schwartz DA (1980) Review: helminths in the induction of cancer: Opisthorchis viverrini, Clonorchis sinensis and cholangiocarcinoma. Trop Geogr Med 32:95–100
  PubMed CAS Google Scholar
• WHO (1995) Control of foodborne trematode infections. WHO Technical Report Series 849, Geneva, Switzerland

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Acknowledgments

We are grateful to Dr. Boris Romashov (Voronezh Biosphere Reserve, Grafskaya, Russia) for providing samples of O. felineus and P. truncatum. We thank Prof. Dr. Han-Jong Rim and Prof. Dr. IC Song (Department of Parasitology, Institute for Tropical Endemic Diseases, College of Medicine, Korea University) for kindly providing C. sinensis and host snails, and we also thank Prof. Dr. Paiboon Sithithaworn (University of Khon Kaen, Faculty of Medicine, Thailand) for providing O. viverrini. Furthermore, we thank Prof. Dr. Rolf Schuster (Central Veterinary Research Laboratory, Dubai, United Arab Emirates) for his help in collecting samples and providing helpful comments. The study received financial support from the European Commission (TREMKIT ICA4-CT-2001-10080).

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Authors and Affiliations

1. Department of Zoomorphology, Cell Biology and Parasitology, Heinrich-Heine University Duesseldorf, Dusseldorf, Germany
   Boris Müller, Jürgen Schmidt & Heinz Mehlhorn

Authors

1. Boris Müller
2. Jürgen Schmidt
3. Heinz Mehlhorn

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Correspondence toHeinz Mehlhorn.

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Müller, B., Schmidt, J. & Mehlhorn, H. Sensitive and species-specific detection of Clonorchis sinensis by PCR in infected snails and fishes.Parasitol Res 100, 911–914 (2007). https://doi.org/10.1007/s00436-006-0322-9

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• Received: 16 August 2006
• Accepted: 22 August 2006
• Published: 12 October 2006
• Issue date: March 2007
• DOI: https://doi.org/10.1007/s00436-006-0322-9

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