Discovery of Paragonimus westermani in Vietnam and its molecular phylogenetic status in P. westermani complex (original) (raw)

Abstract

Paragonimus westermani is the most well-known species among the genus Paragonimus. It is widely distributed in Asia with considerable genetic diversity to form P. westermani species complex. While P. westermani distributed in Japan, Korea, China, and Taiwan are genetically homogeneous to form the East Asia group, those found in other geographic areas are heterogeneous and would be divided into several groups. Recent discoveries of P. westermani in India and Sri Lanka highlighted new insights on molecular phylogenetic relationship of geographic isolates of this species complex. Since Vietnam is located at the east end of Southeast Asia, the intermediate position between South and East Asia, it is of interest to see whether P. westermani is distributed in this country. Here, we report that P. westermani metacercariae were found in mountainous crabs, Potamiscus sp_._, collected in Quangtri province in the central Vietnam. Adult worms were successfully obtained by experimental infection in cats. Molecular phylogenetic analyses revealed that P. westermani of Vietnamese isolates have high similarities with those of East Asia group.

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Acknowledgments

This study was supported by the Japanese Society for Promotion of Science (JSPS) under Ronpaku Fellowship No. NCST-10430 for Pham Ngoc Doanh and in part by the Project for Zoonoses Education and Research, Miyazaki University. Special thanks were sent to Dr. David Blair, School of Marine and Tropical Biology, James Cook University and Dr. Ayako Yoshida, Faculty of Medicine, University of Miyazaki for their useful advice and constructive discussion.

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Authors and Affiliations

1. Department of Parasitology, Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology, Hanoi, Vietnam
   Pham Ngoc Doanh
2. Department of Bio-resources, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan
   Akio Shinohara
3. Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture, University of Miyazaki, Gakuen-Kibanadai-Nishi, Miyazaki, 889-2192, Japan
   Yoichiro Horii
4. Departement of Parasitology, Fukuoka University School of Medicine, Fukuoka, Japan
   Shigehisa Habe
5. Facultad de Ciencias Químco Biológicas, Universidad Autónoma de Sinaloa, Culiacán, Mexico
   Yukifumi Nawa

Authors

1. Pham Ngoc Doanh
2. Akio Shinohara
3. Yoichiro Horii
4. Shigehisa Habe
5. Yukifumi Nawa

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Correspondence toYoichiro Horii.

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Doanh, P.N., Shinohara, A., Horii, Y. et al. Discovery of Paragonimus westermani in Vietnam and its molecular phylogenetic status in P. westermani complex.Parasitol Res 104, 1149–1155 (2009). https://doi.org/10.1007/s00436-008-1302-z

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• Received: 18 November 2008
• Accepted: 26 November 2008
• Published: 13 December 2008
• Issue date: April 2009
• DOI: https://doi.org/10.1007/s00436-008-1302-z

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