Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia (original) (raw)

Abstract

Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation–immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8% of samples contained low numbers of Giardia cysts (range, 0.17–1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.

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Acknowledgement

The authors are very grateful to Mr. Wan Hafiz WI for technical assistance during sample collection.

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Authors and Affiliations

1. Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
   Yvonne A. L. Lim, Mohammed A. K. Mahdy & Wan Yusoff W. Sulaiman
2. Department of Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
   Sharmila D. Ramasame
3. Department of Parasitology, Faculty of Medicine, Sana’a University, Sana’a, Yemen
   Mohammed A. K. Mahdy
4. Scottish Parasite Diagnostic Laboratory, Stobhill Hospital, Glasgow, G21 3UW, Scotland, UK
   Huw V. Smith

Authors

1. Yvonne A. L. Lim
2. Sharmila D. Ramasame
3. Mohammed A. K. Mahdy
4. Wan Yusoff W. Sulaiman
5. Huw V. Smith

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Correspondence toYvonne A. L. Lim.

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Lim, Y.A.L., Ramasame, S.D., Mahdy, M.A.K. et al. Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia.Parasitol Res 106, 289–291 (2009). https://doi.org/10.1007/s00436-009-1602-y

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• Received: 12 November 2008
• Accepted: 12 August 2009
• Published: 25 August 2009
• Issue date: December 2009
• DOI: https://doi.org/10.1007/s00436-009-1602-y

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