Genetic characterization of Echinostoma revolutum and Echinoparyphium recurvatum (Trematoda: Echinostomatidae) in Thailand and phylogenetic relationships with other isolates inferred by ITS1 sequence (original) (raw)

Abstract

Echinostomatidae are common, widely distributed intestinal parasites causing significant disease in both animals and humans worldwide. In spite of their importance, the taxonomy of these echinostomes is still controversial. The taxonomic status of two species, Echinostoma revolutum and Echinoparyphium recurvatum, which commonly infect poultry and other birds, as well as human, is problematical. Previous phylogenetic analyses of Southeast Asian strains indicate that these species cluster as sister taxa. In the present study, the first internal transcribed spacer (ITS1) sequence was used for genetic characterization and to examine the phylogenetic relationships between an isolate from Thailand with other isolates available from GenBank database. Interspecies differences in ITS1 sequence between E. revolutum and E. recurvatum were detected at 6 (3%) of the 203 alignment positions. Of these, nucleotide deletion at positions 25, 26, and 27, pyrimidine transition at 50, 189, and pyrimidine transversion at 118 were observed. Phylogenetic analysis revealed that E. recurvatum from Thailand clustered as a sister taxa with E. revolutum and not with other members of the genus Echinoparyphium. Interestingly, this result confirms a previous report based on allozyme electrophoresis and mitochondrial DNA that E. revolutum and E. recurvatum in Southeast Asia are sister species. Hence, the taxonomic status of E. recurvatum in Thailand, as well as in Southeast Asian countries needs to be confirmed and revised using more comprehensive analyses based on morphology and other molecular techniques.

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Acknowledgments

This research was financially supported by Mahasarakham University and TRF-CHE (grant no. MRG5180102 to Weerachai Saijuntha). We wish to acknowledge the Faculty of Medicine, Khon Kaen University for an Overseas Visiting Professor Programme grant. Thanks to Ms. Kunyarat Duenngai, Mr. Weerasak Saijuntha, Mr. Jatupon Saijuntha for helping with sample collection, and Dr. Anchalee Techasen for the DNA extraction.

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Authors and Affiliations

  1. Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Maha Sarakham, 44150, Thailand
    Weerachai Saijuntha
  2. Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani, 12121, Thailand
    Chairat Tantrawatpan
  3. Department of Parasitology, Liver Fluke and Cholangiocarcinoma Research Centre (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
    Paiboon Sithithaworn & Ross H. Andrews
  4. Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Kornblumen Strasse 13, Karlsruhe, Germany
    Trevor N. Petney

Authors

  1. Weerachai Saijuntha
  2. Chairat Tantrawatpan
  3. Paiboon Sithithaworn
  4. Ross H. Andrews
  5. Trevor N. Petney

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Correspondence toChairat Tantrawatpan.

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Saijuntha, W., Tantrawatpan, C., Sithithaworn, P. et al. Genetic characterization of Echinostoma revolutum and Echinoparyphium recurvatum (Trematoda: Echinostomatidae) in Thailand and phylogenetic relationships with other isolates inferred by ITS1 sequence.Parasitol Res 108, 751–755 (2011). https://doi.org/10.1007/s00436-010-2180-8

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