Characterization of microRNAs in Taenia saginata of zoonotic significance by Solexa deep sequencing and bioinformatics analysis (original) (raw)

Abstract

The beef tapeworm Taenia saginata infects human beings with symptoms ranging from nausea, abdominal discomfort to digestive disturbances and intestinal blockage. In the present study, microRNA (miRNA) expressing profile in adult T. saginata was analyzed using Solexa deep sequencing and bioinformatics analysis. A total of 15.8 million reads was obtained by Solexa sequencing, and 13.3 million clean reads (1.73 million unique sequences) was obtained after removing reads smaller than 18 nt. Ten conserved miRNAs corresponding to 607,382 reads were found when matching the reads against known miRNAs of Schistosoma japonicum in miRBase database. The miR-71 had the most abundant expression in T. saginata, followed by miR-219-5p, but some other common miRNAs such as let-7, miR-40, and miR-103 were not identified in T. saginata. Nucleotide bias analysis found that the known miRNAs showed high bias and the uracil was the dominant nucleotide, particularly at the first and 11th positions which were almost at the beginning and middle of conserved miRNAs. One novel miRNA (Tsa-miR-001) corresponding to ten precursors was identified and confirmed by stem-loop RT-PCR. To our knowledge, this is the first report of miRNA profiles in T. saginata, which will contribute to better understanding of the complex biology of this zoonotic trematode. The reported data of T. saginata miRNAs should provide valuable references for miRNA studies of closed related zoonotic Taenia cestodes such as Taenia solium and Taenia asiatica.

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Acknowledgments

This work is supported in part by the Program for the International Development Research Centre, Canada (Project No. 105509-00001002), National S & T Major Program (grant nos. 2012ZX10004220, 2008ZX10004-011, 2009ZX10004-302 and 2009ZX10004-201), National Key Technology R & D Program (Grant No. 2008BAI56B03), and the Open Funds of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (grant nos. SKLVEB2009KFKT008, SKLVEB2010KFKT009, SKLVEB2011KFKT004, and SKLVEB2011KFKT010). The experiments comply with the current laws of the country in which the experiments were performed.

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Authors and Affiliations

1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, 200025, Peoples Republic of China
   L. Ai, M. X. Chen, Y. N. Zhang, S. H. Chen, J. Guo, Y. C. Cai, X. N. Zhou & J. X. Chen
2. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province, 730046, Peoples Republic of China
   L. Ai, M. J. Xu, M. X. Chen & X. Q. Zhu
3. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, Peoples Republic of China
   X. Q. Zhu
4. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, 650201, Peoples Republic of China
   X. Q. Zhu

Authors

1. L. Ai
2. M. J. Xu
3. M. X. Chen
4. Y. N. Zhang
5. S. H. Chen
6. J. Guo
7. Y. C. Cai
8. X. N. Zhou
9. X. Q. Zhu
10. J. X. Chen

Corresponding authors

Correspondence toX. Q. Zhu or J. X. Chen.

Additional information

L. Ai and M. J. Xu contributed equally to this work.

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Ai, L., Xu, M.J., Chen, M.X. et al. Characterization of microRNAs in Taenia saginata of zoonotic significance by Solexa deep sequencing and bioinformatics analysis.Parasitol Res 110, 2373–2378 (2012). https://doi.org/10.1007/s00436-011-2773-x

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• Received: 23 May 2011
• Accepted: 07 December 2011
• Published: 28 December 2011
• Issue date: June 2012
• DOI: https://doi.org/10.1007/s00436-011-2773-x

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