Prevalence and genotypes of Giardia duodenalis from dogs in Spain: possible zoonotic transmission and public health importance (original) (raw)

Abstract

The prevalence of Giardia duodenalis was determinate in faecal samples from dogs and cats in Madrid, Spain and molecular characterisation of isolates. A total of 604 and 144 faecal samples from dogs and cats, respectively, were analysed by routine coprological methods. The prevalence of G. duodenalis was 16.4 % (99/604) in dogs and 4.2 % (6/144) in cats. Sixty-four G. duodenalis isolates (63 from dogs and 1 from a cat) were characterised using glutamate dehydrogenase and β-giardin genes by PCR-RFLP. The single cat sample showed a mixed infection by assemblages A + F. The assemblages found in the dog samples were A, B, C, D and E, both as single and as mixed infections. The zoonotic assemblages A and B were found in 56 (88.8 %) _G. duodenalis_-positive samples with 15.9 % of samples having assemblage A (10/63) and 73 % of samples with assemblage B (46/63), indicating high potential zoonotic risk and public health significance.

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Acknowledgments

The present study was partly funded by grant FIS PI 10/01240 from the Ministerio de Economía y Competitividad, Spain.

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Authors and Affiliations

1. Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040, Madrid, Spain
   Diana Dado, Ana Montoya & Guadalupe Miró
2. Servicio de Parasitología, Centro Nacional de Microbiología, ISCIII, Carretera de Pozuelo-Majadahonda km 2, 28224, Majadahonda, Madrid, Spain
   Diana Dado, María Alejandra Blanco, José María Saugar, Begoña Bailo & Isabel Fuentes
3. Departamento de Microbiología y Parasitología, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida, Venezuela
   María Alejandra Blanco

Authors

1. Diana Dado
2. Ana Montoya
3. María Alejandra Blanco
4. Guadalupe Miró
5. José María Saugar
6. Begoña Bailo
7. Isabel Fuentes

Corresponding author

Correspondence toIsabel Fuentes.

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Dado, D., Montoya, A., Blanco, M.A. et al. Prevalence and genotypes of Giardia duodenalis from dogs in Spain: possible zoonotic transmission and public health importance.Parasitol Res 111, 2419–2422 (2012). https://doi.org/10.1007/s00436-012-3100-x

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• Received: 30 July 2012
• Accepted: 22 August 2012
• Published: 15 September 2012
• Issue date: December 2012
• DOI: https://doi.org/10.1007/s00436-012-3100-x

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