Morphological and molecular characterization of Eustrongylides excisus larvae (Nematoda: Dioctophymatidae) in Sander lucioperca (L.) from Northern Turkey (original) (raw)

Abstract

The genus Eustrongylides Jägerskiöld, 1909 includes parasitic nematodes (Dioctophymatidae) affecting various fish species and piscivorous birds of freshwater ecosystems. Currently, there is little information on the molecular characterization of E. excisus based on nuclear ribosomal internal transcribed spacer (ITS) rDNA regions. However, before the present study, there had been no reports of characterizing the E. excisus using nuclear small subunit ribosomal RNA (SSU rRNA) and mitochondrial cytochrome c oxidase subunit I (COI) genes sequences. In the present study, Eustrongylides spp. larvae were collected from pike-perch Sander lucioperca (L.) in Northern Turkey, and characterized by sequencing of ITS regions, SSU rRNA, and COI markers. Larvae herein morphologically identified as the fourth stage of Eustrongylides spp. were genetically identified as E. excisus based on the ITS sequence analysis. This study is the first record of SSU rRNA and COI sequences for E. excisus in GenBank. This is also a molecular characterization of E. excisus for the first time in Turkey. The ITS, SSU rRNA, and COI sequences of E. excisus can be used to establish the phylogenetic relationships of Eustrongylides species from Turkey and worldwide for further studies.

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References

• Abe N (2011) Molecular and morphological identification of helminthes found in Japanese smelt, Hypomesus transpacificus nipponensis, with notes on new host records of Eustrongylides ignotus and Raphidascaris gigi. Acta Parasitol 56:227–231
  Article Google Scholar
• Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
  Article CAS Google Scholar
• Bjelić-Čabrilo O, Novakov N, Ćirković M, Kostić D, Popović E, Aleksić N, Lujić J (2013) The first determination of Eustrongylides excisus Jägerskiöld, 1909: Larvae (Nematoda: Dioctophymatidae) in the pike-perch Sander lucioperca in Vojvodina (Serbia). Helminthologia 50:291–294
  Article Google Scholar
• Branciari R, Ranucci D, Miraglia D, Valiani A, Veronesi F, Urbani E, Lo Vaglio G, Pascucci L, Franceschini R (2016) Occurrence of parasites of the genus Eustrongylides spp (Nematoda Dioctophymatidae) in fish caught in Trasimeno Lake Italy. Ital J Food Saf 5:6130
  PubMed PubMed Central Google Scholar
• Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552
  Article CAS Google Scholar
• Dezfuli BS, Manera M, Lorenzoni M, Pironi F, Shinn AP, Giari L (2015) Histopathology and the inflammatory response of European perch Perca fluviatilis muscle infected with Eustrongylides sp (Nematoda). Parasit Vectors 8:227
  Article Google Scholar
• Goncharov SL, Soroka NM, Pashkevich IY, Dubovy AI, Bondar AO (2018) Infection of predatory fish with larvae of Eustrongylides excisus (Nematoda, Dioctophymatidae) in the Delta of the Dnipro River and the Dnipro-Buh Estuary in Southern Ukraine. Vestn Zool 52:137–144
  Article Google Scholar
• Guagliardo S, Viozzi G, Brugni N (2019) Pathology associated with larval Eustrongylides sp. (Nematoda: Dioctophymatoidea) infection in Galaxias maculatus (Actinopterygii: Galaxiidae) from Patagonia. Argentina Int J Parasitol Parasites Wildl 10:113–116
  Article Google Scholar
• Guardone L, Ricci E, Susini F, Polsinelli E, Guglielmone G, Armani A (2021) First detection of Eustrongylides excisus (Nematoda: Dioctophymatidae) in big-scale sand smelt (Atherina boyeri) from the lake Massaciuccoli (Northwest Tuscany, Italy): implications for public health and seafood quality. Food Control 120:107517
  Article CAS Google Scholar
• Hasegawa H, Sato A, Kai M, Uchida A (2013) Helminth parasites of bullfrogs, Lithobates catesbeianus (Shaw, 1802), in Kanto District, Japan, with special reference to those introduced from North America. Jpn J Vet Parasitol 12:1–10
  Google Scholar
• Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Mentjies P, Drummond A (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649
  Article Google Scholar
• Koehler AV, Hoberg EP, Torres-Pérez F, Cook JA (2009) A molecular view of the superfamily Dioctophymatoidea (Nematoda). Comp Parasitol 76:100–104
  Article Google Scholar
• Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549
  Article CAS Google Scholar
• Kuraiem BP, Knoff M, Telleria EL, Fonseca MCG, Machado LS, Cunha NC, Nascimento ER, Fontenelle G, Gomes DC, São Clemente SC (2020) Eustrongylides sp (Nematoda Dioctophymatoidea) parasitizing Hoplias malabaricus (Actinopterygii Erythrinidae) collected from the state of Rio de Janeiro Brazil. Braz J Vet Parasitol 29:e014519
  Article CAS Google Scholar
• Mazzone A, Caffara M, Gustinelli A, Agnetti F, Sgariglia E, Vaglio GL, Quaglio F, Fioravanti ML (2019) Morphological and molecular characterization of larval and adult stages of Eustrongylides excisus (Nematoda: Dioctophymatoidea) with histopathological observations. J Parasitol 105:882–889
  Article Google Scholar
• Measures LN (1988) Revision of the genus Eustrongylides Jägerskiöld, 1909 (Nematoda: Dioctophymatoidea) of piscivorous birds. Can J Zool 66:885–895
  Article Google Scholar
• Metin S, Didinen BI, Boyaci YO, Kubilay A, Emre N, Didinen H, Emre Y (2014) Occurrence of Eustrongylides excisus Jägerskiöld, 1909 larvae (Nematoda: Dioctophymatidae) in Pikeperch (Sander lucioperca, L.) in Lake Eğirdir. Acta Aqua Tr 10:20–24
  Google Scholar
• Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
  Article CAS Google Scholar
• Xiong F, Li WX, Wu SG, Zou H, Wang GT (2013) Molecular phylogeny and host specificity of the larval Eustrongylides (Nematoda: Dioctophmidae) from freshwater fish in China. J Parasitol 99:137–144
  Article CAS Google Scholar
• Youssefi MR, Tabaripour R, Hosseini M (2020) Molecular characterisation and histopathological study of Eustrongylides excisus nematode in the northern pike (Esox lucius). Bulg J Vet Med. https://doi.org/10.15547/bjvm.2392
  Article Google Scholar

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Acknowledgements

This work was supported by the Scientific Research Council of Ondokuz Mayis University, Samsun, Turkey under project no PYO.VET.1901.16.006.

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Authors and Affiliations

1. Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55139, Samsun, Turkey
   Gokmen Zafer Pekmezci
2. Department of Parasitology, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55139, Samsun, Turkey
   Cenk Soner Bolukbas

Authors

1. Gokmen Zafer Pekmezci
2. Cenk Soner Bolukbas

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Correspondence toGokmen Zafer Pekmezci.

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Pekmezci, G.Z., Bolukbas, C.S. Morphological and molecular characterization of Eustrongylides excisus larvae (Nematoda: Dioctophymatidae) in Sander lucioperca (L.) from Northern Turkey.Parasitol Res 120, 2269–2274 (2021). https://doi.org/10.1007/s00436-021-07187-8

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• Received: 23 December 2020
• Accepted: 05 May 2021
• Published: 18 May 2021
• Version of record: 18 May 2021
• Issue date: June 2021
• DOI: https://doi.org/10.1007/s00436-021-07187-8

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