A soluble 12-kDa protein of the mitochondrial intermembrane space, Mrs11p, is essential for mitochondrial biogenesis and viability of yeast cells (original) (raw)

Abstract

We have isolated an essential yeast gene, termed MRS11, which codes for a soluble protein of the mitochondrial intermembrane space. Interestingly, this new gene shares many similarities with the previously characterized MRS5 gene: when expressed from a multicopy plasmid, MRS11 like MRS5 restores respiration competence to yeast strains defective in the splicing of mitochondrial group II introns. Both genes are essential for viability of yeast cells, as the disruption of either of them is lethal. The proteins encoded by MRS5 and MRS11, which display 35%, sequence identity are both located in the mitochondrial intermembrane space. Depletion of Mrs11p results in a phenotype similar to that observed in Mrs5p-depleted cells: accumulation of the precursor form of mitochondrial hsp60, inability to form spectrophotometrically detectable amounts of cytochromes and changes in the mitochondrial morphology. Although similar in sequence and function, Mrs5p and Mrs11p are not functionally equivalent and neither can substitute for the other, even when overexpressed. Taken together, our data suggest a cooperative mode of action of Mrs11p and Mrs5p in mitochondrial protein import or other related essential mitochondrial processes.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Vienna Biocenter, Institute of Microbiology and Genetics, University of Vienna, A-1030 Vienna, Austria Email: schweyen@gem.univie.ac.at, , , , , , AT
    E. Jarosch & R. J. Schweyen
  2. Institute of Genetics, Technical University of Dresden, D-01062 Dresden, Germany, , , , , , DE
    G. Rödel

Authors

  1. E. Jarosch
    You can also search for this author inPubMed Google Scholar
  2. G. Rödel
    You can also search for this author inPubMed Google Scholar
  3. R. J. Schweyen
    You can also search for this author inPubMed Google Scholar

Additional information

Received: 14 January 1997 / Accepted: 18 March 1997

Rights and permissions

About this article

Cite this article

Jarosch, E., Rödel, G. & Schweyen, R. A soluble 12-kDa protein of the mitochondrial intermembrane space, Mrs11p, is essential for mitochondrial biogenesis and viability of yeast cells.Mol Gen Genet 255, 157–165 (1997). https://doi.org/10.1007/s004380050484

Download citation

Keywords