Budding yeast Rad50, Mre11, Xrs2, and Hdf1, but not Rad52, are involved in the formation of deletions on a dicentric plasmid (original) (raw)

Abstract

We have previously shown that the RAD50, RAD52, MRE11, XRS2, and HDF1 genes of Saccharomyces cervisiae are involved in the formation of deletions by illegitimate recombination on a monocentric plasmid. In this study, we investigated the effects of mutations of these genes on formation of deletions of a dicentric plasmid, in which DNA double-strand breaks are expected to occur frequently because the two centromeres are pulled to opposite poles in mitosis. We transformed yeast cells with a dicentric plasmid, and after incubation for a few division cycles, cells carrying deleted plasmids were detected using negative selection markers. Deletions occurred at a higher frequency than on the monocentric plasmid and there were short regions of homology at the recombination junctions as observed on the monocentric plasmid. In rad50, mre11, xrs2, and hdf1 mutants, the frequency of occurrence of deletions was reduced by about 50-fold, while in the rad52 mutant, it was comparable to that in the wild-type strain. The end-joining functions of Rad50, Mre11, Xrs2, and Hdf1, suggest that these proteins play important roles in the joining of DNA ends produced on the dicentric plasmid during mitosis.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Molecular Biology, Institute of Medical Science, University of Tokyo, P.O. Takanawa, Tokyo 108, Japan e-mail: ike@hgc.ims.u-tokyo.ac.jp, , , , , , JP
    Y. Tsukamoto, J. Kato & H. Ikeda

Authors

  1. Y. Tsukamoto
    You can also search for this author inPubMed Google Scholar
  2. J. Kato
    You can also search for this author inPubMed Google Scholar
  3. H. Ikeda
    You can also search for this author inPubMed Google Scholar

Additional information

Received: 30 October 1996 / Accepted: 28 February 1997

Rights and permissions

About this article

Cite this article

Tsukamoto, Y., Kato, J. & Ikeda, H. Budding yeast Rad50, Mre11, Xrs2, and Hdf1, but not Rad52, are involved in the formation of deletions on a dicentric plasmid.Mol Gen Genet 255, 543–547 (1997). https://doi.org/10.1007/s004380050527

Download citation