DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase (original) (raw)
Abstract
The Cdc14 protein encodes a dual-specificity protein phosphatase which functions in late mitosis, and considerable genetic evidence suggests a role in DNA replication. We find that cdc14 mutants arrested in late mitosis maintain persistent levels of mitotic kinase activity, suggesting that Cdc14 controls inactivation of this kinase. Overexpression of Sic1, a cyclin-dependent protein kinase inhibitor, is able to suppress telophase mutants such as dbf2, cdc5 and cdc15, but not cdc14. It does, however, force _cdc14_-arrested cells into the next cell cycle, in which an apparently normal S phase occurs as judged by FACS and pulsed-field gel electrophoretic analysis. Furthermore, in a promoter shut-off experiment, cells lacking Cdc14 appear to carry out a normal S phase. Thus Cdc14 functions mainly in late mitosis and it has no essential role in S phase.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
Author information
Authors and Affiliations
- Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA. UK e-mail: l-johnst@nimr.mrc.ac.uk, , , , , , GB
P. J. Fitzpatrick, J. H. Toyn, J. B. A. Millar & L. H. Johnston
Authors
- P. J. Fitzpatrick
You can also search for this author inPubMed Google Scholar - J. H. Toyn
You can also search for this author inPubMed Google Scholar - J. B. A. Millar
You can also search for this author inPubMed Google Scholar - L. H. Johnston
You can also search for this author inPubMed Google Scholar
Additional information
Received: 9 January 1998 / Accepted: 22 January 1998
Rights and permissions
About this article
Cite this article
Fitzpatrick, P., Toyn, J., Millar, J. et al. DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase.Mol Gen Genet 258, 437–441 (1998). https://doi.org/10.1007/s004380050753
- Issue Date: May 1998
- DOI: https://doi.org/10.1007/s004380050753