Quantification by flow cytometry of chromosome-17 deletions in Smith-Magenis syndrome patients (original) (raw)

Abstract

We have used bivariate flow karyotyping to quantify the deletions involving chromosome 17 in sixteen patients with Smith-Magenis syndrome (SMS). The fluorescence intensities of mitotic chromosomes stained with Hoechst 33258 and chromomycin were quantified in a dual-beam flow cytometer. For each patient, the position of the peak representing the deleted chromosome 17 was compared to those of the normal homologs of an unaffected parent. The patients could be classified into four groups based on the size of their deletions. The deletions ranged from ∼9–10 Mb (∼10–11% of the chromosome) to below the detection limit of the technique (2 Mb). Different deletion sizes were detected among patients whose high-resolution banding results were similar. Some deletions detected by banding were not detected by flow analyses. Deletion estimates are largely consistent with the results of molecular analyses. Patients with larger deletions that extend into band 17p12 have abnormal electrophysiologic studies of peripheral nerves. Deletion size does not appear to correlate with the degree of mental retardation, presence of behavioral abnormalities, craniofacial anomalies or common skeletal findings in SMS. By identifying patients with varying deletion sizes, these data will aid the construction of a long-range deletion-based map of 17p11.2 and identification of the genes involved in this syndrome.

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Authors and Affiliations

1. Department of Molecular Biotechnology, Box 357730, University of Washington, Seattle, WA 98195, USA, , , , , , US
   B. J. Trask, Heather Mefford, Ger van den Engh & Hillary F. Massa
2. Genetic Counseling Center, Rhode Island Hospital, Providence, RI 02903, USA, , , , , , IS
   Dianne N. Abuelo
3. Child Development Rehabilitation Center, Oregon Health Sciences University, Portland, OR 97201-2998, USA, , , , , , US
   Ellen Magenis
4. Department of Neurology, Baylor College of Medicine, Houston, TX 77030, USA, , , , , , US
   Ramesh C. Juyal & P. I. Patel
5. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA, , , , , , US
   Lorraine Potocki, Antonio Baldini, Frank Greenberg & James R. Lupski
6. Elwyn, Inc., Elwyn, PA 19063, USA, , , , , , US
   Brenda Finucane
7. Department of Genetics, Kaiser Permanente, San Jose, CA 95119, USA, , , , , , US
   David R. Witt

Authors

1. B. J. Trask
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2. Heather Mefford
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3. Ger van den Engh
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4. Hillary F. Massa
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5. Ramesh C. Juyal
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6. Lorraine Potocki
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7. Brenda Finucane
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8. Dianne N. Abuelo
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9. David R. Witt
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10. Ellen Magenis
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11. Antonio Baldini
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12. Frank Greenberg
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13. James R. Lupski
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14. P. I. Patel
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Received: 19 March 1996 / Revised: 21 June 1996

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Trask, B., Mefford, H., van den Engh, G. et al. Quantification by flow cytometry of chromosome-17 deletions in Smith-Magenis syndrome patients.Hum Genet 98, 710–718 (1996). https://doi.org/10.1007/s004390050291

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• Issue Date: November 1996
• DOI: https://doi.org/10.1007/s004390050291

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