Asynchronous replication of p53 and 21q22 loci in chronic lymphocytic leukemia (original) (raw)

Abstract

In this study, in order to evaluate the replication pattern and the cell cycle dynamics of normal and malignant cells from patients with chronic lymphocytic leukemia, we applied the FISH technique with the p53 gene. Asynchrony was determined by the presence of one single and one set of double dots in the same cell. The rate of asynchronous replication was significantly higher in malignant cells than in normal cells (a mean of 28 vs 13, respectively, P = 0.023). There were proportionately more cells with two single dots among the normal cells (P = 0.0047). These results probably reflect the changes in gene replication and cell cycle progression that occur in malignant cells.

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Authors and Affiliations

  1. Genetic Institute, Meir General Hospital, Kfar Saba, Israel 44281 Tel.: +972 9 7472540; Fax: +972 9 7419851, , , , , , IL
    A. Amiel, Elena Gaber & Moshe D. Fejgin
  2. Department of Human Genetics, Tel Aviv University Medical School, Israel, , , , , , IL
    Tali Litmanovich & Lydia Avivi
  3. Department of Medicine ‘A’, Meir Hospital, Kfar Saba, Israel, , , , , , IL
    Michael Lishner

Authors

  1. A. Amiel
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  2. Tali Litmanovich
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  3. Elena Gaber
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  4. Michael Lishner
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  5. Lydia Avivi
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  6. Moshe D. Fejgin
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Received: 25 March 1997 / Accepted: 28 July 1997

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Amiel, A., Litmanovich, T., Gaber, E. et al. Asynchronous replication of p53 and 21q22 loci in chronic lymphocytic leukemia.Hum Genet 101, 219–222 (1997). https://doi.org/10.1007/s004390050619

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