Polysome-like structures in the chromatoid body of rat spermatids (original) (raw)

Abstract

A procedure for isolating the chromatoid body from the testis of 40-day-old rats was developed. Electron-microscopical analysis indicated that about 70% of the isolated organelles were chromatoid bodies, while the remaining structures corresponded to dense bodies and probably to satellites. Negative staining of the isolated organelles revealed the presence of polysome-like structures in about 20% of the chromatoid bodies suggesting that the polysomes were not due to contamination with cytoplasmic polysomes. Moreover, the presence of RNA in the stroma of the chromatoid body was confirmed by RNAse-gold staining. Preliminary electrophoretic analysis of the RNA extracted from the organelles revealed the presence of a complex population of RNAs including 5.8 and 5 S ribosomal RNAs but no tRNA.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile, , , , , , CL
    Jaime Figueroa & Luis O. Burzio

Authors

  1. Jaime Figueroa
    You can also search for this author inPubMed Google Scholar
  2. Luis O. Burzio
    You can also search for this author inPubMed Google Scholar

Additional information

Received: 10 March 1997 / Accepted: 7 October 1997

Rights and permissions

About this article

Cite this article

Figueroa, J., Burzio, L. Polysome-like structures in the chromatoid body of rat spermatids.Cell Tissue Res 291, 575–579 (1998). https://doi.org/10.1007/s004410051027

Download citation