The Expression Patterns of Toll-Like Receptors in the Ileal Pouch Mucosa of Postoperative Ulcerative Colitis Patients (original) (raw)

Abstract

The aim of this study was to evaluate the expression pattern of Toll-like receptors (TLRs) in the pouch mucosa of ulcerative colitis patients in comparison with that in the ileum mucosa of noninflammatory bowel disease patients. Pouch mucosal biopsy specimens were collected from postoperative patients who had undergone surgery for ulcerative colitis. Normal ileum specimens were collected from colon cancer patients. The specimens were assessed by immunofluorescence histochemistry using TLR2, TLR3, TLR4, and TLR5 polyclonal antibodies. The normal ileal mucosa constitutively expressed TLR3 and TLR5, whereas TLR2 and TLR4 were barely detectable. In the mucosa of active pouchitis, TLR2 and TLR4 was strongly upregulated, and TLR4 was upregulated even in a noninflamed pouch. No TLR3 or TLR5 expression was detectable. These data suggest that pouchitis may be associated with distinctive changes in selective TLR expression in the pouch mucosa, and that TLR4 alterations in the innate response system may contribute to the pathogenesis of these disorders in particular.

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References

  1. DA Macafee JF Abercrombie C Maxwell-Armstrong (2004)ArticleTitlePouchitis_Colorectal Dis_ 6 142–52Occurrence Handle10.1111/j.1463-1318.2004.00645.xOccurrence Handle1:STN:280:DC%2BD2c3ht1Ohtw%3D%3DOccurrence Handle15109376
    Article CAS PubMed Google Scholar
  2. MC Rubinstein RL Fisher (1996)ArticleTitlePouchitis: pathogenesis, diagnosis, and management_Gastroenterologist_ 4 129–33Occurrence Handle1:STN:280:BymA1cvisVI%3DOccurrence Handle8792143
    CAS PubMed Google Scholar
  3. DG Nasmyth PG Godwin MF Dixon NS Williams D Johnston (1989)ArticleTitleIleal ecology after pouch-anal anastomosis or ileostomy. A study of mucosal morphology, fecal bacteriology, fecal volatile fatty acids, and their interrelationship_Gastroenterology_ 96 817–24Occurrence Handle1:STN:280:BiaC387ktVQ%3DOccurrence Handle2914643
    CAS PubMed Google Scholar
  4. R Medzhitov P Preston-Hurlburt CA JanewaySuffixJr (1997)ArticleTitleA human homologue of the Drosophila Toll protein signals activation of adaptive immunity_Nature_ 24 394–7
    Google Scholar
  5. O Takeuchi K Hoshino T Kawai H Sanjo H Takada T Ogawa_et al._ (1999)ArticleTitleDifferential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components_Immunity_ 11 443–51Occurrence Handle10.1016/S1074-7613(00)80119-3Occurrence Handle1:CAS:528:DyaK1MXntFCnur4%3DOccurrence Handle10549626
    Article CAS PubMed Google Scholar
  6. L Alexopoulou AC Holt R Medzhitov RA Flavell (2001)ArticleTitleRecognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3_Nature_ 413 732–8Occurrence Handle10.1038/35099560Occurrence Handle1:CAS:528:DC%2BD3MXnvFegsL0%3DOccurrence Handle11607032
    Article CAS PubMed Google Scholar
  7. E Cario DK Podolsky (2000)ArticleTitleDifferential alteration in intestinal epithelial cell expression of toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease_Infect Immun_ 68 7010–7Occurrence Handle10.1128/IAI.68.12.7010-7017.2000Occurrence Handle1:CAS:528:DC%2BD3MXjslCku7w%3DOccurrence Handle11083826
    Article CAS PubMed Google Scholar
  8. M Hausmann S Kiessling S Mestermann G Webb T Spottl T Andus_et al._ (2002)ArticleTitleToll-like receptors 2 and 4 are up-regulated during intestinal inflammation_Gastroenterology_ 122 1987–2000Occurrence Handle10.1053/gast.2002.33662Occurrence Handle1:CAS:528:DC%2BD38Xlt12gsLY%3DOccurrence Handle12055604
    Article CAS PubMed Google Scholar
  9. S Naik EJ Kelly L Meijer S Pettersson IR Sanderson (2001)ArticleTitleAbsence of Toll-like receptor 4 explains endotoxin hyporesponsiveness in human intestinal epithelium_J Pediatr Gastroenterol Nutr_ 32 449–53Occurrence Handle10.1097/00005176-200104000-00011Occurrence Handle1:CAS:528:DC%2BD3MXkslGktbs%3DOccurrence Handle11396812
    Article CAS PubMed Google Scholar
  10. AT Gewirtz TA Navas S Lyons PJ Godowski JL Madara (2001)ArticleTitleCutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression_J Immunol_ 167 1882–5Occurrence Handle1:CAS:528:DC%2BD3MXlvFeqt7Y%3DOccurrence Handle11489966
    CAS PubMed Google Scholar
  11. G Sebastiani G Leveque L Lariviere L Laroche E Skamene P Gros_et al._ (2000)ArticleTitleCloning and characterization of the murine toll-like receptor 5 (Tlr5) gene: sequence and mRNA expression studies in Salmonella-susceptible MOLF/Ei mice_Genomics_ 15 IssueID64 230–40
    Google Scholar
  12. TG Wolfs WA Buurman A van Schadewijk B de Vries MA Daemen PS Hiemstra_et al._ (2002)ArticleTitleIn vivo expression of Toll-like receptor 2 and 4 by renal epithelial cells: IFN-gamma and TNF-alpha mediated up-regulation during inflammation_J Immunol_ 168 1286–93Occurrence Handle1:CAS:528:DC%2BD38XpsFeltA%3D%3DOccurrence Handle11801667
    CAS PubMed Google Scholar
  13. F Nomura S Akashi Y Sakao S Sato T Kawai M Matsumoto_et al._ (2000)ArticleTitleCutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with downregulation of surface toll-like receptor 4 expression_J Immunol_ 164 3476–9Occurrence Handle1:CAS:528:DC%2BD3cXitFOqsrw%3DOccurrence Handle10725699
    CAS PubMed Google Scholar
  14. R Duchmann I Kaiser E Hermann W Mayet K Ewe KH Meyer zum Buschenfelde (1995)ArticleTitleTolerance exists towards resident intestinal flora but is broken in active inflammatory bowel disease (IBD)Clin Exp Immunol 102 448–55Occurrence Handle1:STN:280:BymC38fhtlU%3DOccurrence Handle8536356
    CAS PubMed Google Scholar

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Authors and Affiliations

  1. Second Department of Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan
    Yuji Toiyama, Toshimitsu Araki, Shigeyuki Yoshiyama, Jun-ichiro Hiro, Chikao Miki & Masato Kusunoki

Authors

  1. Yuji Toiyama
  2. Toshimitsu Araki
  3. Shigeyuki Yoshiyama
  4. Jun-ichiro Hiro
  5. Chikao Miki
  6. Masato Kusunoki

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Toiyama, Y., Araki, T., Yoshiyama, S. et al. The Expression Patterns of Toll-Like Receptors in the Ileal Pouch Mucosa of Postoperative Ulcerative Colitis Patients.Surg Today 36, 287–290 (2006). https://doi.org/10.1007/s00595-005-3144-y

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