Characterization of Kaposi’s sarcoma-associated herpesvirus/human herpesvirus-8 ORF57 promoter (original) (raw)

Summary.

Kaposi’s sarcoma-associated herpesvirus (KSHV) is a recently discovered human gamma herpesvirus (HHV-8) that plays an important role in Kaposi’s sarcoma development. Here, we further characterize the regulation of the early HHV-8 gene, open reading frame 57 (ORF57). ORF57 is a spliced gene consisting of two exons with a 108-bp intron near the 5′ end. The ORF57 mRNA can potentially be initiated at two different start sites, and its expression can be significantly stimulated by ORF50, an HHV-8 immediate early gene. The target site for ORF50 transactivation was mapped to a 40-bp fragment compassing nt 81904 to 81943 in the ORF57 promoter. Our study on the regulation of ORF57 expression by ORF50 provides the basis for further studies on the regulation of HHV-8 lytic gene expression.

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Authors and Affiliations

  1. School of Biological Sciences, University of Nebraska, Lincoln, Nebraska, U.S.A., , , , , , US
    S. Wang, S. Liu & C. Wood
  2. Center for Biological Chemistry, University of Nebraska, Lincoln, Nebraska, U.S.A., , , , , , US
    W. Duan

Authors

  1. W. Duan
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  2. S. Wang
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  3. S. Liu
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  4. C. Wood
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Received October 28, 1999 Accepted June 22, 2000

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Duan, W., Wang, S., Liu, S. et al. Characterization of Kaposi’s sarcoma-associated herpesvirus/human herpesvirus-8 ORF57 promoter.Arch. Virol. 146, 403–413 (2001). https://doi.org/10.1007/s007050170185

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