Expression of dentin matrix protein 1 (DMP1) in nonmineralized tissues (original) (raw)

Abstract

Dentin matrix protein 1 (DMP1) is an Arg-Gly-Asp-containing acidic phosphoprotein that was originally identified from a rat incisor cDNA library and was thought to be a dentin-specific protein. DMP1 was later shown to express in a number of hard tissue-forming cells, including osteoblasts, osteocytes, ameloblasts, and cementoblasts, and was considered to play important roles in mineralization. Further, DMP1 gene expression was also detected in fetal bovine brain and in newborn mouse brain. These findings indicate the possibility of DMP1 expression in other soft tissues. In the present study, to clarify the significance of DMP1 expression in nonmineralized tissues, we made a specific antibody to mouse DMP1 peptides and demonstrated that DMP1 protein was localized in mouse brain, pancreas, and kidney by immunohistochemistry. Further DMP1 mRNA was detected in nonmineralized mouse tissues including liver, muscle, brain, pancreas, and kidney by RT-PCR. Based on the evidence that the localization and the expression of DMP1 are not restricted to mineralized tissues, we assume that DMP1 may have functions other than the regulation of mineralization.

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Authors and Affiliations

  1. Developmental Oral Health Science, Department of Orofacial Development and Function, Division of Oral Health Science, Tokyo Medical and Dental University, Tokyo, Japan
    Makiko Terasawa & Yuzo Takagi
  2. Neuropathology, Department of Brain Medical Science, Division of Cognitive and Behavioral Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    Reiko Shimokawa
  3. Biostructural Science, Department of Hard Tissue Engineering, Division of Bio-Matrix, Tokyo Medical and Dental University, Tokyo, Japan
    Tatsuo Terashima
  4. Pharmacology, Department of Hard Tissue Engineering, Division of Bio-matrix, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan
    Keiichi Ohya & Hitoyata Shimokawa

Authors

  1. Makiko Terasawa
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  2. Reiko Shimokawa
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  3. Tatsuo Terashima
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  4. Keiichi Ohya
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  5. Yuzo Takagi
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  6. Hitoyata Shimokawa
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Corresponding author

Correspondence toHitoyata Shimokawa.

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Cite this article

Terasawa, M., Shimokawa, R., Terashima, T. et al. Expression of dentin matrix protein 1 (DMP1) in nonmineralized tissues.J Bone Miner Metab 22, 430–438 (2004). https://doi.org/10.1007/s00774-004-0504-4

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