Epidemiological Typing of Bordetella pertussis Isolates: Recommendations for a Standard Methodology (original) (raw)

Abstract

Pertussis is re-emerging in vaccinated populations, and to gain insight into the reasons for this development population-based studies are necessary. Unfortunately, various techniques are used to study Bordetella pertussis populations, hampering comparison between studies. A standard methodology for epidemiological typing of Bordetella pertussis isolates is proposed which is based on serotyping, pulsed-field gel electrophoresis and gene typing. Such a standard approach will allow comparisons between studies performed in different laboratories. Comparisons may reveal whether the epidemiological differences observed between countries are due for instance to different Bordetella pertussis populations or different vaccines used.

Access this article

Log in via an institution

Subscribe and save

• Starting from 10 chapters or articles per month
• Access and download chapters and articles from more than 300k books and 2,500 journals
• Cancel anytime View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

1. Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands e-mail: fr.mooi@rivm.nl, , , , , , NL
   F. R. Mooi
2. Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden e-mail: hans.hallander@smi.ki.se, , , , , , SE
   H. Hallander
3. Institut für Hygiene und Labormedizin, Klinikum Krefeld, Lutherplatz 40, 47805 Krefeld, Germany e-mail: wvk_hyg@klinikum-krefeld.de, , , , , , DE
   C.H. Wirsing von König
4. SmithKline Beecham Biologicals, rue de l'Institut 89, 1330 Rixensart, Belgium e-mail: bernard.hoet@SBBio.be, , , , , , BE
   B. Hoet
5. Laboratoire des Bordetella, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France e-mail: nguiso@pasteur.fr, , , , , , FR
   N. Guiso

Authors

1. F. R. Mooi
2. H. Hallander
3. C.H. Wirsing von König
4. B. Hoet
5. N. Guiso

Rights and permissions

About this article

Cite this article

Mooi, F., Hallander, H., Wirsing von König, C. et al. Epidemiological Typing of Bordetella pertussis Isolates: Recommendations for a Standard Methodology.EJCMID 19, 174–181 (2000). https://doi.org/10.1007/s100960050455

Download citation

• Issue date: April 2000
• DOI: https://doi.org/10.1007/s100960050455

Keywords