Effects of low-level laser therapy (685 nm) at different doses in osteogenic cell cultures (original) (raw)

Abstract

The present in vitro study evaluated parameters of osteogenesis under the influence of low-level laser therapy (LLLT) at different doses. Osteogenic cells originated from rat calvaria were cultivated in polystyrene plates and exposed to a laser irradiation using an indium-gallium-aluminum phosphide therapeutic laser (InGaAIP), at wavelength of 685 nm, power of 35 mW, 600-μm-diameter optical fiber, and continuous wave. In the attempt of observing the existence of a dose response and its effects, laser irradiation was performed at 25, 77, and 130 J/cm2 (7, 22, and 37 s, respectively). The following parameters were assessed: growth curve (4, 7, and 11 days), cell viability (24 h), and nodular formation of mineralized matrix (14 days). The results did not show significant differences related to the growth curve (4, 7, and 11 days) and cell viability (24 h). Within 14 days, osteogenic cultures showed nodular areas with well-defined calcified matrix. The total area stained with Alizarin Red did not show any differences between doses of 25 and 130 J/cm2. However, the percentage of stained area was significantly higher in the 25 J/cm2 group when compared to the group of 77 J/cm2 (Kruskal-Wallis test, p < 0.05). It was possible to conclude that the 685-nm laser irradiation (at 25, 77, and 130 J/cm2) did not influence cell growth and proliferation, although the extracellular mineralization process may have its pattern altered by the LLLT on osteogenic cell cultures.

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References

  1. Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31(3):334–340
    Article PubMed CAS Google Scholar
  2. Pinheiro AL (2009) Advances and perspectives on tissue repair and healing. Photomed Laser Surg 27(6):833–836
    Article PubMed Google Scholar
  3. Mester E, Mester AF, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5(1):31–39
    Article PubMed CAS Google Scholar
  4. Kamali F, Bayat M, Torkaman G, Ebrahimi E, Salavati M (2007) The therapeutic effect of low-level laser on repair of osteochondral defects in rabbit knee. J Photochem Photobiol B 88(1):11–15
    Article PubMed CAS Google Scholar
  5. Khadra M, Kasem N, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Laser therapy accelerates initial attachment and subsequent behaviour of human oral fibroblasts cultured on titanium implant material. A scanning electron microscope and histomorphometric analysis. Clin Oral Implants Res 16(2):168–175
    Article PubMed Google Scholar
  6. Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterials 26(17):3503–3509
    Article PubMed CAS Google Scholar
  7. Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Determining optimal dose of laser therapy for attachment and proliferation of human oral fibroblasts cultured on titanium implant material. J Biomed Mater Res A 73(1):55–62
    PubMed Google Scholar
  8. Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23(2):161–166
    Article PubMed CAS Google Scholar
  9. Conlan MJ, Rapley JW, Cobb CM (1996) Biostimulation of wound healing by low-energy laser irradiation. A review. J Clin Periodontol 23(5):492–496
    Article PubMed CAS Google Scholar
  10. Kujawa J, Zavodnik L, Zavodnik I, Buko V, Lapshyna A, Bryszewska M (2004) Effect of low-intensity (3.75-25 J/cm2) near-infrared (810 nm) laser radiation on red blood cell ATPase activities and membrane structure. J Clin Laser Med Surg 22(2):111–117
    Article PubMed Google Scholar
  11. Xu M, Deng T, Mo F, Deng B, Lam W, Deng P, Zhang X, Liu S (2009) Low-intensity pulsed laser irradiation affects RANKL and OPG mRNA expression in rat calvarial cells. Photomed Laser Surg 27(2):309–315
    Article PubMed CAS Google Scholar
  12. Pires Oliveira DA, de Oliveira RF, Zangaro RA, Soares CP (2008) Evaluation of low-level laser therapy of osteoblastic cells. Photomed Laser Surg 26(4):401–404
    Article PubMed Google Scholar
  13. Fukuhara E, Goto T, Matayoshi T, Kobayashi S, Takahashi T (2006) Optimal low-energy laser irradiation causes temporal G2/M arrest on rat calvarial osteoblasts. Calcif Tissue Int 79(6):443–450
    Article PubMed CAS Google Scholar
  14. Renno AC, McDonnell PA, Parizotto NA, Laakso EL (2007) The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25(4):275–280
    Article PubMed CAS Google Scholar
  15. Schlager A, Kronberger P, Petschke F, Ulmer H (2000) Low-power laser light in the healing of burns: a comparison between two different wavelengths (635 nm and 690 nm) and a placebo group. Lasers Surg Med 27(1):39–42
    Article PubMed CAS Google Scholar
  16. Silva JC, Lacava ZG, Kuckelhaus S, Silva LP, Neto LF, Sauro EE, Tedesco AC (2004) Evaluation of the use of low-level laser and photosensitizer drugs in healing. Lasers Surg Med 34(5):451–457
    Article PubMed Google Scholar
  17. Peplow PV, Chung TY, Baxter GD (2010) Laser photobiomodulation of proliferation of cells in culture: a review of human and animal studies. Photomed Laser Surg 28(Suppl 1):S3–S40
    PubMed Google Scholar
  18. Peplow PV, Chung TY, Baxter GD (2010) Laser photobiomodulation of wound healing: a review of experimental studies in mouse and rat animal models. Photomed Laser Surg 28(3):291–325
    Article PubMed Google Scholar
  19. Bellows CG, Aubin JE, Heersche JN, Antosz ME (1986) Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif Tissue Int 38(3):143–154
    Article PubMed CAS Google Scholar
  20. Aubin JE (1998) Advances in the osteoblast lineage. Biochem Cell Biol 76(6):899–910
    Article PubMed CAS Google Scholar
  21. Nanci A, Zalzal S, Gotoh Y, McKee MD (1996) Ultrastructural characterization and immunolocalization of osteopontin in rat calvarial osteoblast primary cultures. Microsc Res Tech 33(2):214–231
    Article PubMed CAS Google Scholar
  22. de Oliveira PT, Zalzal SF, Irie K, Nanci A (2003) Early expression of bone matrix proteins in osteogenic cell cultures. J Histochem Cytochem 51(5):633–641
    Article PubMed Google Scholar
  23. Stein E, Koehn J, Sutter W, Wendtlandt G, Wanschitz F, Thurnher D, Baghestanian M, Turhani D (2008) Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells. Wien Klin Wochenschr 120(3–4):112–117
    Article PubMed CAS Google Scholar
  24. Rosa AL, Beloti MM (2003) Rat bone marrow cell response to titanium and titanium alloy with different surface roughness. Clin Oral Implants Res 14(1):43–48
    Article PubMed Google Scholar
  25. Li WT, Leu YC, Wu JL (2010) Red-light light-emitting diode irradiation increases the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells. Photomed Laser Surg 28(Suppl 1):S157–S165
    PubMed CAS Google Scholar
  26. Renno AC, McDonnell PA, Crovace MC, Zanotto ED, Laakso L (2010) Effect of 830 nm laser phototherapy on osteoblasts grown in vitro on biosilicate scaffolds. Photomed Laser Surg 28(1):131–133
    Article PubMed CAS Google Scholar
  27. Fujimoto K, Kiyosaki T, Mitsui N, Mayahara K, Omasa S, Suzuki N, Shimizu N (2010) Low-intensity laser irradiation stimulates mineralization via increased BMPs in MC3T3-E1 cells. Lasers Surg Med 42(6):519–526
    Article PubMed Google Scholar
  28. Kim IS, Cho TH, Kim K, Weber FE, Hwang SJ (2010) High power-pulsed Nd:YAG laser as a new stimulus to induce BMP-2 expression in MC3T3-E1 osteoblasts. Lasers Surg Med 42(6):510–518
    Article PubMed Google Scholar
  29. Chellini F, Sassoli C, Nosi D, Deledda C, Tonelli P, Zecchi-Orlandini S, Formigli L, Giannelli M (2010) Low pulse energy Nd:YAG laser irradiation exerts a biostimulative effect on different cells of the oral microenvironment: "An in vitro study". Lasers Surg Med 42(6):527–539
    Article PubMed Google Scholar
  30. Khadra M, Ronold HJ, Lyngstadaas SP, Ellingsen JE, Haanaes HR (2004) Low-level laser therapy stimulates bone-implant interaction: An experimental study in rabbits. Clin Oral Implants Res 15(3):325–332
    Article PubMed Google Scholar
  31. Dortbudak O, Haas R, Mallath-Pokorny G (2000) Biostimulation of bone marrow cells with a diode soft laser. Clin Oral Implants Res 11(6):540–545
    Article PubMed CAS Google Scholar

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Acknowledgements

This study had the financial support of the Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil). The authors would like to thank Professor Paulo Tambasco de Oliveira and Roger Rodrigo Fernandes (from Universidade de São Paulo, Ribeirão Preto, Brazil) for technical assistance.

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Authors and Affiliations

  1. Division of Periodontology, Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP, São Paulo State University, Araraquara, SP, Brazil
    Humberto Osvaldo Schwartz-Filho, Aline C. Reimer, Claudio Marcantonio, Elcio Marcantonio Jr. & Rosemary Adriana C. Marcantonio
  2. Faculdade de Odontologia de Araraquara UNESP, Rua Humaitá 1680, 14801–903, Araraquara, SP, Brazil
    Humberto Osvaldo Schwartz-Filho

Authors

  1. Humberto Osvaldo Schwartz-Filho
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  2. Aline C. Reimer
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  3. Claudio Marcantonio
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  4. Elcio Marcantonio Jr.
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  5. Rosemary Adriana C. Marcantonio
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Correspondence toHumberto Osvaldo Schwartz-Filho.

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Schwartz-Filho, H.O., Reimer, A.C., Marcantonio, C. et al. Effects of low-level laser therapy (685 nm) at different doses in osteogenic cell cultures.Lasers Med Sci 26, 539–543 (2011). https://doi.org/10.1007/s10103-011-0902-5

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