Low-level laser therapy vs. pulsed electromagnetic field on neonatal rat calvarial osteoblast-like cells (original) (raw)

Abstract

To compare the effects of pulsed electromagnetic field (PEMF) and low-level laser therapy (LLLT) on osteoblast cells in a cell culture model. Fifty thousand neonatal rat calvarial osteoblast-like cells per milliliter were seeded and 0.06 mT PEMF, 0.2 mT PEMF, and LLLT at 808 nm were applied for 24 and 96 h on the cells. To evaluate cellular proliferation and differentiation, specimens were examined for DNA synthesis, alkaline phosphatase (ALP) activity, cell numbers, and viability of the cells. Morphological appearances of the cells were observed using scanning electron microcopy after 24 and 96 h of incubation. At 24 and 96 h, the control group had a higher cell proliferation than 0.06 and 0.2 mT PEMF groups (p = 0.001). At 96 h, 0.2 mT PEMF group had higher cell proliferation rate than 0.06 mT PEMF and LLLT groups (p = 0.001). The cell count and cell viability in 0.2 mT PEMF group were higher than the 0.06-mT PEMF and LLLT groups, although these differences were not statistically significant at 96 h (p > 0.05). At 24 and 96 h, cell viability in the control group was higher than the test groups. Alkaline phosphatase levels of the groups were comparable in both time intervals (p > 0.05). 0.2 mT PEMF application on osteoblast-like cells led to cell proliferation and differentiation better than 0.06 mT PEMF and LLLT at 808 nm, although a remarkable effect of both PEMF and LLLT could not be detected. The ALP activity of 0.2 and 0.06 mT PEMF and LLLT were comparable.

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Acknowledgments

We would like to thank Selhan Kaya DDS, Ph.D. and Banu Korkmaz DDS, for their support to the present study

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Istanbul University, Istanbul, Turkey
    Yusuf Emes, Buket Aybar, Serhat Yalçın & Belir Atalay
  2. Department of Prosthodontics, Faculty of Dentistry, Hacettepe University, Sihhiye, Ankara, Turkey
    Kivanç Akça & Yeliz Çavuşoğlu
  3. Department of Electrical and Electronics Engineeering, Faculty of Engineering, Hacettepe University, Ankara, Turkey
    Uğur Baysal
  4. Department of Public Health, Faculty of Medicine, Istanbul University, Istanbul, Turkey
    Halim Işsever
  5. Department of Biochemistry, Faculty of Medicine, Istanbul University, Istanbul, Turkey
    Pervin Vural
  6. Department of Biochemistry, Faculty of Medicine, Yeni Yüzyıl University, Istanbul, Turkey
    Mine Ergüven
  7. Department of Prosthodontics, Faculty of Dentistry, Ordu University, Ordu, Turkey
    Murat Cavit Çehreli
  8. Department of Histology and Embriology, Faculty of Medicine, Istanbul University, Istanbul, Turkey
    Ayhan Bilir
  9. Department of Oral Surgery, Faculty of Dentistry, Istanbul University, Çapa/İstanbul, 34093, Turkey
    Buket Aybar

Authors

  1. Yusuf Emes
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  2. Kivanç Akça
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  3. Buket Aybar
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  4. Serhat Yalçın
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  5. Yeliz Çavuşoğlu
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  6. Uğur Baysal
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  7. Halim Işsever
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  8. Belir Atalay
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  9. Pervin Vural
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  10. Mine Ergüven
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  11. Murat Cavit Çehreli
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  12. Ayhan Bilir
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Corresponding author

Correspondence toBuket Aybar.

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Emes, Y., Akça, K., Aybar, B. et al. Low-level laser therapy vs. pulsed electromagnetic field on neonatal rat calvarial osteoblast-like cells.Lasers Med Sci 28, 901–909 (2013). https://doi.org/10.1007/s10103-012-1165-5

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