Decreased paraoxonase activity in critically ill patients with sepsis (original) (raw)

Abstract

Paraoxonase 1 is believed to play a role in preventing lipid oxidation and, thus, limiting production of proinflammatory mediators. Systemic inflammatory response in sepsis increases oxidative stress and decreases high-density lipoprotein (HDL) concentrations. The objective of this study was to investigate serum paraoxonase 1 activities in critically ill patients with sepsis and after recovery. Serum paraoxonase 1 arylesterase/paraoxonase activities, concentration of total cholesterol, HDL cholesterol (HDL-C) and serum C-reactive protein (CRP) in septic patients of a medical intensive care unit (n = 30) and age/sex-matched outpatient controls without sepsis (n = 30) were analyzed. Paired convalescent samples were also taken 1 week after recovery (n = 11). In septic patients, both arylesterase (88.3 ± 36.5 vs. 162.1 ± 44.8 kU/l, P < 0.001) and paraoxonase (75.2 ± 50.0 vs. 125.2 ± 69.4 U/l, P < 0.01) paraoxonase 1 activities decreased as compared to controls. Both activities normalized after recovery. Negative correlation was found between CRP and both arylesterase (r = −0.676, P < 0.001) and paraoxonase (r = −0.401, P < 0.01) as well as positive correlation between HDL-C and both arylesterase (r = 0.585, P < 0.001) and paraoxonase (r = 0.405, P < 0.01) paraoxonase 1 activities. The decreased activity of paraoxonase 1 in negative correlation with CRP offers a potentially useful marker of sepsis progress and recovery in critically ill patients.

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Acknowledgments

This study was supported by a research grant from the Czech Ministry of Health (Project No. IGANR/8943-4).

Conflict of interest statement

The authors declare that they have no conflict of interest related to the publication of this manuscript.

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Authors and Affiliations

  1. 4th Department of Internal Medicine, 1st Faculty of Medicine, General Teaching Hospital, Charles University in Prague, U Nemocnice 2, CZ-12808, Prague, Czech Republic
    Frantisek Novak, Lucie Vavrova, Jana Kodydkova, Magdalena Hynkova & Ales Zak
  2. Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic
    Frantisek Novak Sr. & Magdalena Hynkova
  3. Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic
    Olga Novakova
  4. Centre for Cardiovascular Research, Prague, Czech Republic
    Frantisek Novak Sr.

Authors

  1. Frantisek Novak
  2. Lucie Vavrova
  3. Jana Kodydkova
  4. Frantisek Novak Sr.
  5. Magdalena Hynkova
  6. Ales Zak
  7. Olga Novakova

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Correspondence toFrantisek Novak.

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Novak, F., Vavrova, L., Kodydkova, J. et al. Decreased paraoxonase activity in critically ill patients with sepsis.Clin Exp Med 10, 21–25 (2010). https://doi.org/10.1007/s10238-009-0059-8

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