AFLP-Based Genetic Diversity Assessment of Commercially Important Tea Germplasm in India (original) (raw)

Abstract

India has a large repository of important tea accessions and, therefore, plays a major role in improving production and quality of tea across the world. Using seven AFLP primer combinations, we analyzed 123 commercially important tea accessions representing major populations in India. The overall genetic similarity recorded was 51%. No significant differences were recorded in average genetic similarity among tea populations cultivated in various geographic regions (northwest 0.60, northeast and south both 0.59). UPGMA cluster analysis grouped the tea accessions according to geographic locations, with a bias toward China or Assam/Cambod types. Cluster analysis results were congruent with principal component analysis. Further, analysis of molecular variance detected a high level of genetic variation (85%) within and limited genetic variation (15%) among the populations, suggesting their origin from a similar genetic pool.

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Acknowledgments

A financial grant received from the Department of Biotechnology and the Council of Scientific and Industrial Research, Government of India, is gratefully acknowledged. We thank Dr. S. Rajkumar for providing help in bootstrap analysis and AMOVA. This is IHBT Publication 0649.

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Authors and Affiliations

1. Biotechnology Division, Institute of Himalayan Bioresource Technology, CSIR, P.O. Box 6, Palampur, 176061, Himachal Pradesh, India
   R. K. Sharma, P. Bhardwaj, R. Kumar & P. S. Ahuja
2. Plant Molecular Biology Division, The Energy Resources Institute, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi, 110003, India
   M. S. Negi, S. B. Tripathi & M. Lakshmikumaran
3. Department of Botany, University of Delhi, Delhi, 110007, India
   S. Sharma, E. Bhattachrya & S. N. Raina
4. UPASI Tea Research Institute, Valparai, Coimbatore, 642127, India
   D. Vijayan, R. Rajkumar, J. Thomas & N. Muraleedharan
5. Tea Research Association, Tocklai Experimental Station, Jorhat, 785008, Assam, India
   A. R. Baruah, S. C. Das, B. Bera & M. Hazarika
6. HATS Division, Institute of Himalayan Bioresource Technology, CSIR, P.O. Box 6, Palampur, 176061, Himachal Pradesh, India
   R. K. Sud

Authors

1. R. K. Sharma
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2. M. S. Negi
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3. S. Sharma
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4. P. Bhardwaj
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5. R. Kumar
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6. E. Bhattachrya
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7. S. B. Tripathi
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8. D. Vijayan
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9. A. R. Baruah
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10. S. C. Das
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11. B. Bera
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12. R. Rajkumar
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13. J. Thomas
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14. R. K. Sud
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15. N. Muraleedharan
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16. M. Hazarika
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17. M. Lakshmikumaran
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18. S. N. Raina
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19. P. S. Ahuja
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Correspondence toR. K. Sharma or P. S. Ahuja.

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Sharma, R.K., Negi, M.S., Sharma, S. et al. AFLP-Based Genetic Diversity Assessment of Commercially Important Tea Germplasm in India.Biochem Genet 48, 549–564 (2010). https://doi.org/10.1007/s10528-010-9338-z

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• Received: 12 August 2009
• Accepted: 19 November 2009
• Published: 14 April 2010
• Issue Date: August 2010
• DOI: https://doi.org/10.1007/s10528-010-9338-z

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