High-level Expression of a Synthetic Gene Encoding a Sweet Protein, Monellin, in Escherichia coli (original) (raw)

Abstract

The expression of a synthetic gene encoding monellin, a sweet protein, in E. coli under the control of T7 promoter from phage is described. The single-chain monellin gene was designed based on the biased codons of E. coli so as to optimize its expression. Monellin was produced and accounted for 45% of total soluble proteins. It was purified to yield 43 mg protein per g dry cell wt. The purity of the recombinant protein was confirmed by SDS-PAGE.

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Authors and Affiliations

1. Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, 300222, Tianjin, P. R. China
   Zhongjun Chen, Heng Cai, Fuping Lu & Lianxiang Du

Authors

1. Zhongjun Chen
2. Heng Cai
3. Fuping Lu
4. Lianxiang Du

Corresponding author

Correspondence toFuping Lu.

Additional information

Revisions requested 13 April 2005 and 26 May 2005; Revisions received 19 May 2005 and 30 August 2005

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Chen, Z., Cai, H., Lu, F. et al. High-level Expression of a Synthetic Gene Encoding a Sweet Protein, Monellin, in Escherichia coli.Biotechnol Lett 27, 1745–1749 (2005). https://doi.org/10.1007/s10529-005-3544-5

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• Received: 15 March 2005
• Accepted: 31 August 2005
• Issue date: November 2005
• DOI: https://doi.org/10.1007/s10529-005-3544-5

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