Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3 (original) (raw)

Abstract

Synthesis of silver nanoparticles using α-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time_._ The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10–25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.

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Acknowledgement

S. Anil Kumar thanks the Council of Scientific and Industrial Research (CSIR), Government of India, for financial assistance. This work was supported by a grant from the Department of Biotechnology, Govt. of India to Dr. M. I. Khan.

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Authors and Affiliations

1. Biochemical Sciences Division, National Chemical Laboratory, Pune, 411 008, India
   S. Anil Kumar, Absar Ahmad & M. I. Khan
2. DST Unit on Nanoscience, Department of Physics, Pune University, Pune, 411 007, India
   Majid Kazemian Abyaneh, S. W. Gosavi & Sulabha K. Kulkarni
3. Materials Chemistry Division, National Chemical Laboratory, Pune, 411 008, India
   Renu Pasricha

Authors

1. S. Anil Kumar
2. Majid Kazemian Abyaneh
3. S. W. Gosavi
4. Sulabha K. Kulkarni
5. Renu Pasricha
6. Absar Ahmad
7. M. I. Khan

Corresponding author

Correspondence toM. I. Khan.

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Anil Kumar, S., Abyaneh, M.K., Gosavi, S.W. et al. Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3 .Biotechnol Lett 29, 439–445 (2007). https://doi.org/10.1007/s10529-006-9256-7

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• Received: 22 September 2006
• Revised: 27 October 2006
• Accepted: 30 October 2006
• Published: 20 January 2007
• Issue date: March 2007
• DOI: https://doi.org/10.1007/s10529-006-9256-7

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