In vivo repair of rat transected sciatic nerve by low-intensity pulsed ultrasound and induced pluripotent stem cells-derived neural crest stem cells (original) (raw)

Abstract

Objectives

To evaluate the effects of the combination of low-intensity pulsed ultrasound (LIPUS) and induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) on the regeneration of rat transected sciatic nerve in vivo.

Results

Tissue-engineered tubular nerve conduit was fabricated by electrospinning aligned nanofibers in longitudinal direction. This sustained the iPSCs-NCSCs and could be used as a bridge in rat transected sciatic nerve. Treatment with 0.3 W cm−2 LIPUS for 2 weeks and 5 min per day significantly improved the sciatic functional index, static sciatic function index and nerve conduction velocity of rat sciatic nerve. Histological analysis showed that there were more regenerative new blood vessels and new neurofilaments, higher expression level of β-III tubulin (Tuj1) in the experimental group seeded with iPSCs-NCSCs and stimulated with LIPUS.

Conclusion

Combination of LIPUS with iPSCs-NCSCs promoted the regeneration and reconstruction of rat transected sciatic nerve and is an efficient and cost-effective method for peripheral nerve regeneration.

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Acknowledgments

This work was supported in part by grants from the National Natural Science Foundation of China (11172338), the Fundamental Research Funds for the Central Universities (CQDXWL-2012-Z001), and the Sharing Fund of Chongqing University’s Large-scale Equipment.

Supporting information

Supplementary experimental methods (Cell culture; Preparation of electrospun solution and electrospinning; Nerve function analysis).

Supplementary Fig. 1—Characteristic of PLLA nanofibers.

Supplementary Fig. 2—Gross observation of the nerve conduits.

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Authors and Affiliations

1. Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People’s Republic of China
   Yonggang Lv, Panpan Nan, Guobao Chen, Yongqiang Sha, Bin Xia & Li Yang
2. ‘111’ Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, People’s Republic of China
   Yonggang Lv, Panpan Nan, Guobao Chen, Yongqiang Sha, Bin Xia & Li Yang
3. Mechanobiology and Regenerative Medicine Laboratory, Bioengineering College, Chongqing University, 174 Shazheng Jie, Shapingba, Chongqing, 400044, People’s Republic of China
   Yonggang Lv

Authors

1. Yonggang Lv
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2. Panpan Nan
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3. Guobao Chen
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4. Yongqiang Sha
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5. Bin Xia
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6. Li Yang
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Corresponding author

Correspondence toYonggang Lv.

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Lv, Y., Nan, P., Chen, G. et al. In vivo repair of rat transected sciatic nerve by low-intensity pulsed ultrasound and induced pluripotent stem cells-derived neural crest stem cells.Biotechnol Lett 37, 2497–2506 (2015). https://doi.org/10.1007/s10529-015-1939-5

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• Received: 14 July 2015
• Accepted: 13 August 2015
• Published: 25 August 2015
• Issue Date: December 2015
• DOI: https://doi.org/10.1007/s10529-015-1939-5

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