Lactobacillus rhamnosus GG microcapsules inhibit Escherichia coli biofilm formation in coculture (original) (raw)
Abstract
Objectives
Microbial biofilms have become one of the most significant causes of nosocomial infections. The aim of this study was to examine the potential quorum sensing inhibitor activities of Lactobacillus rhamnosus GG microcapsules.
Results
Lactobacillus rhamnosus GG microcapsules effectively inhibited initial biofilm formation at a concentration of 2.5 × 108 CFU/mL. Furthermore, the inhibition rate was increased to 79% in the Lactobacillus rhamnosus GG microcapsules group, resulting in a reduction in the biofilm maturation stage. In addition, real-time PCR analysis revealed that the LGG microcapsules can act as effective inhibitors of transcriptional activators of the quorum sensing circuit in E.coli, luxS, lsrK, and lsrR.
Conclusions
Lactobacillus rhamnosus GG microcapsules can effectively inhibit biofilm formation and disturb mature biofilms.
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References
- Algarni AA, Yassen GH, Gregory RL (2015) Inhibitory effects of gels loaded with a low concentration of antibiotics against biofilm formation by Enterococcus faecalis and Porphyromonas gingivalis. J Oral Sci 57:213–218
Article CAS Google Scholar - Capurso L (2019) Thirty years of Lactobacillus rhamnosus GG: a review. J Clin Gastroenterol Suppl 1:S1–S41
Google Scholar - Cook MT, Tzortzis G, Charalampopoulos D et al (2012) Microencapsulation of probiotics for gastrointestinal delivery. J Control Release 162:56–67
Article CAS PubMed Google Scholar - Dekeersmaecker SC, Vanderleyden J (2003) Constraints on detection of autoinducer-2 (AI-2) signaling molecules using Vibrio harveyi as a reporter. J Microbiol 149:1953–1956
Article CAS Google Scholar - Ding WY, Sun J, Lian H et al (2018) The influence of shuttle-shape emodin nanoparticles on the Streptococcus suis biofilm. Front Pharmacol 9:227
Article CAS PubMed PubMed Central Google Scholar - Gao M, Song HY, Zheng HZ et al (2016a) Culture of low density E. coli cells in alginate-chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system. Carbohydr Polym 141:160–165
Article CAS PubMed Google Scholar - Gao M, Song HY, Liu XD et al (2016b) Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules. J Biosci Bioeng 121:406–412
Article CAS PubMed Google Scholar - Guerin J, Burgain J, Borges F et al (2017) Use of imaging techniques to identify efficient controlled release systems of Lactobacillus rhamnosus GG during in vitro digestion. Food Funct 8:1587–1598
Article CAS PubMed Google Scholar - Khan MS, Zahin M, Hasan S et al (2009) Inhibition of quorum sensing regulated bacterial functions by plant essential oils with special reference to clove oil. Lett Appl Microbiol 49:354–360
Article CAS PubMed Google Scholar - Kotzé M, Eloff JN (2002) Extraction of antibacterial compound from Combretum microphyllum (Combretaceae). S Afr J Bot 68:62–67
Article Google Scholar - Li HD, Li XY, Wang ZL et al (2015) Autoinducer-2 regulates Pseudomonas aeruginosa PAO1 biofilm formation and virulence production in a dose-dependent manner. BMC Microbiol 15:192
Article CAS PubMed PubMed Central Google Scholar - Marques CNH, Davies DG, Sauer K (2015) Control of biofilms with the fatty acid signaling molecule _cis_-2-decenoic acid. Pharmaceuticals 8:816–835
Article CAS PubMed PubMed Central Google Scholar - Matsuda Y, Cho O, Sugita T et al (2018) Culture supernatants of Lactobacillus gasseri and L. crispatus inhibit Candida albicans biofilm formation and adhesion to HeLa cells. Mycopathologia 183:691–700
Article CAS PubMed Google Scholar - Mehta DK, Das R (2018) Microbial biofilm and quorum sensing inhibition: endowment of medicinal plants to combat multidrug-resistant bacteria. Curr Drug Targets 19:1916–1932
Article CAS PubMed Google Scholar - Novais JS, Moreira CS, Silva ACJA et al (2018) Antibacterial naphthoquinone derivatives targeting resistant strain Gram-negative bacteria in biofilms. Microb Pathog 118:105–114
Article CAS PubMed Google Scholar - Petrova MI, Imholz NC, Verhoeven TL et al (2016) Lectin-like molecules of Lactobacillus rhamnosus GG inhibit pathogenic Escherichia coli and Salmonella biofilm formation. PLoS ONE 11:e0161337
Article CAS PubMed PubMed Central Google Scholar - Radzig MA, Nadtochenko VA, Koksharova OA et al (2013) Antibacterial effects of silver nanoparticles on gram-negative bacteria: influence on the growth and biofilms formation, mechanisms of action. Colloids Surf B 102:300–306
Article CAS Google Scholar - Song HY, Yu WT, Liu XD et al (2014) Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells. Carbohydr Polym 108:10–16
Article CAS PubMed Google Scholar - Spacova I, Lievens E, Verhoeven T et al (2018) Expression of fluorescent proteins in Lactobacillus rhamnosus to study host-microbe and microbe-microbe interactions. Microb Biotechnol 11:317–331
Article CAS PubMed Google Scholar - Vieco-Saiz N, Belguesmia Y, Raspoet R et al (2019) Benefits and inputs from lactic acid bacteria and their bacteriocins as alternatives to antibiotic growth promoters during food-animal production. Front Microbiol 11:57
Article Google Scholar - Wang L, Li J, March JC et al (2005) _luxS_-dependent gene regulation in Escherichia coli K-12 revealed by genomic expression profiling. J Bacteriol 187:8350–8360
Article CAS PubMed PubMed Central Google Scholar - Xue WM, Yu WT, Liu XD et al (2004) Chemical method of breaking the cell-loaded sodium alginate/chitosan microcapsule. Chem J Chin Univ 25:1342–1346
CAS Google Scholar
Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant No. 81601734), Liaoning Provincial Natural Science Foundation of China (Grant No. 20180530062) and a Basic Research Projects Grant of the Key Laboratory of the Education Department of Liaoning Province (Grant No. LZ2015028).
Supporting information
Supplementary Table 1―PCR primer sequences.
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Authors and Affiliations
- Clinical Laboratory of Integrative Medicine, First Hospital Affiliated to Dalian Medical University, 222 Zhongshan Road, Dalian, 116023, Liaoning, People’s Republic of China
Huiyi Song, Jialin Qu, Jianjun Liu, Peiyuan Yin, Guixin Zhang & Dong Shang - College of Pharmacy, Dalian Medical University, Dalian, Liaoning, People’s Republic of China
Jianbin Zhang - The Third Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, People’s Republic of China
Guixin Zhang & Dong Shang
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- Huiyi Song
You can also search for this author inPubMed Google Scholar - Jianbin Zhang
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You can also search for this author inPubMed Google Scholar - Guixin Zhang
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Correspondence toGuixin Zhang or Dong Shang.
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Song, H., Zhang, J., Qu, J. et al. Lactobacillus rhamnosus GG microcapsules inhibit Escherichia coli biofilm formation in coculture.Biotechnol Lett 41, 1007–1014 (2019). https://doi.org/10.1007/s10529-019-02694-2
- Received: 01 January 2019
- Accepted: 16 May 2019
- Published: 30 May 2019
- Issue Date: 01 September 2019
- DOI: https://doi.org/10.1007/s10529-019-02694-2