Characterization of CENH3 and centromere-associated DNA sequences in sugarcane (original) (raw)

• 697 Accesses
• 68 Citations
• 9 Altmetric
• Explore all metrics

Abstract

Centromere-specific histone H3 (CENH3) has been used to detect active centromeres, and to analyse the DNA sequences closely associated with the centromere, because they localize only in active centromeres and bind directly to the DNA. In maize and rice, the centromeric retrotransposons (CR) are shown to be closely associated with their own CENH3 whereas no such association was found in Arabidopsis thaliana. In this study, this sort of association was investigated in sugarcane. Two expressed sequence tag groups encoding putative sugarcane CENH3 (SoCENH3) were found in a sugarcane-expressed sequence tag database. Their deduced amino acid sequences were similar to these of the CENH3s in rice and maize. An antibody against rice CENH3 seemed to crossreact with the SoCENH3s, and stained sugarcane centromeres. A set of immunoprecipitation tests was conducted with the antibody and chromatin from the sugarcane genome to reveal CENH3-associated DNA sequences in sugarcane. Centromeric tandem repeats (SCEN) and centromeric retrotransposons of sugarcane (CRS) were significantly precipitated with the antibody, meaning these repeats are directly interacting with CENH3 in sugarcane centromeres.

Access this article

Log in via an institution

Subscribe and save

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

1. Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan
   Kiyotaka Nagaki & Minoru Murata
2. CREST, Japan Science and Technology Agency, Kawaguchi, 332-0012, Japan
   Kiyotaka Nagaki & Minoru Murata

Authors

1. Kiyotaka Nagaki
   You can also search for this author inPubMed Google Scholar
2. Minoru Murata
   You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toKiyotaka Nagaki.

Rights and permissions

About this article

Cite this article

Nagaki, K., Murata, M. Characterization of CENH3 and centromere-associated DNA sequences in sugarcane.Chromosome Res 13, 195–203 (2005). https://doi.org/10.1007/s10577-005-0847-2

Download citation

• Received: 22 October 2004
• Revised: 22 October 2004
• Accepted: 13 January 2005
• Issue Date: February 2005
• DOI: https://doi.org/10.1007/s10577-005-0847-2

Key words