Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA) (original) (raw)

Abstract

We report 11 novel microsatellite primer pairs for the wild tea, Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura_._ These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and C. japonica. The number of alleles ranged from 4 to18. The expected (H E) and observed (H O) heterozygosity were 0.687–0.946 and 0.042–0.792, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species. Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure in wild tea.

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Acknowledgment

The study was supported by a grant of the National Cheng Kung University.

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Authors and Affiliations

1. Department of Life Sciences, Cheng-Kung University, 1, University Road, Tainan, 701, Taiwan
   Cheng-Yu Hung, Kuo-Hsiung Wang, Chi-Chun Huang & Tzen-Yuh Chiang
2. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, P.R. China
   Xun Gong
3. State Key Laboratory of Biocontrol School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, P.R. China
   Xue-Jun Ge

Authors

1. Cheng-Yu Hung
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2. Kuo-Hsiung Wang
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3. Chi-Chun Huang
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4. Xun Gong
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5. Xue-Jun Ge
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6. Tzen-Yuh Chiang
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Correspondence toTzen-Yuh Chiang.

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Hung, CY., Wang, KH., Huang, CC. et al. Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA).Conserv Genet 9, 779–781 (2008). https://doi.org/10.1007/s10592-007-9391-2

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• Received: 06 July 2007
• Accepted: 10 July 2007
• Published: 02 August 2007
• Issue Date: June 2008
• DOI: https://doi.org/10.1007/s10592-007-9391-2

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