Serum miR-181b Is Correlated with Hepatitis B Virus Replication and Disease Progression in Chronic Hepatitis B Patients (original) (raw)

Abstract

Background

Previously, we reported that microRNA-181b (miR-181b) activates hepatic stellate cells partly through the phosphatase and tensin homolog deleted on chromosome 10 (PTEN)/Akt pathway.

Aims

The main objective of this study was to ascertain whether serum miR-181b expression is correlated with that of liver hepatitis B virus (HBV) DNA and disease progression in chronic hepatitis B (CHB) patients.

Methods

Serum miR-181b and liver HBV DNA levels were quantified in 64 CHB patients with real-time PCR. Liver fibrosis and necroinflammation were graded according to the Ishak scoring system.

Results

Serum miR-181b levels were evaluated in the CHB group, compared with healthy controls. Expression in patients with HBsAg (+) was higher than that in patients with HBsAg (−). Notably, serum miR-181b and liver HBV DNA levels were significantly correlated (P < 0.05). Serum miR-181 levels were higher in patients with serum HBV DNA > 103 IU/ml (P = 0.017), histologic activity index (HAI) >8 (P = 0.001) and fibrosis score >4 (P < 0.0001). Liver HBV DNA levels were higher in patients with abnormal alanine aminotransferase (ALT) values (_P_ = 0.004), serum HBV DNA levels > 103 IU/ml (P = 0.034) and fibrosis score >4 (P = 0.006). Using multivariate logistic regression analysis, serum miR-181b was identified as an independent predictor of disease progression (OR 4.172, 95 % CI 1.838–9.473, P = 0.009 for HAI >8; OR 5.387, 95 % CI 2.067–14.036, P = 0.001 for fibrosis score >4).

Conclusions

Serum miR-181b is correlated with liver and serum HBV DNA levels as well as disease progression in CHB.

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Acknowledgments

The project was supported by National Natural Science Foundation of China (81000176/H0317, 81100292/H0317), Zhejiang Provincial Natural Science Foundation of China (Y2090326, Y2110634) and Wang Bao-En Liver Fibrosis Foundation (Nos. 20100002, 20120127).

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Infectious Diseases, The First Affiliated Hospital of Wenzhou Medical University, No. 2 FuXue Lane, Wenzhou, 325000, Zhejiang, People’s Republic of China
    Fujun Yu & Peihong Dong
  2. Department of Infectious Diseases, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325000, People’s Republic of China
    Guangyao Zhou
  3. Department of Hepatology, The Second Yinzhou Hospital, Ningbo, 315000, People’s Republic of China
    Guojun Li
  4. Wenzhou Key Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University, No. 2 FuXue Lane, Wenzhou, 325000, Zhejiang, People’s Republic of China
    Bicheng Chen & Jianjian Zheng

Authors

  1. Fujun Yu
  2. Guangyao Zhou
  3. Guojun Li
  4. Bicheng Chen
  5. Peihong Dong
  6. Jianjian Zheng

Corresponding authors

Correspondence toPeihong Dong or Jianjian Zheng.

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Yu, F., Zhou, G., Li, G. et al. Serum miR-181b Is Correlated with Hepatitis B Virus Replication and Disease Progression in Chronic Hepatitis B Patients.Dig Dis Sci 60, 2346–2352 (2015). https://doi.org/10.1007/s10620-015-3649-1

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