Assessment and rationalization of genetic diversity of Papua New Guinea taro (Colocasia esculenta) using SSR DNA fingerprinting (original) (raw)

Abstract

Taro (Colocasia esculenta) accessions were collected from 15 provinces of Papua New Guinea (PNG). The collection, totalling 859 accessions was collated for characterization and a core collection of 81 accessions (∼10%) was established on the basis of characterization data generated on 30 agro-morphological descriptors, and DNA fingerprinting using seven SSR primers. The selection of accessions was based on cluster analysis of the morphological data enabling initial selection of 20% accessions. The 20% sample was then reduced and rationalized to 10% based on molecular data generated by SSR primers. This represents the first national core collection of any species established in PNG based on molecular markers. The core has been integrated with core from other Pacific Island countries, contributing to a Pacific regional core collection, which is conserved in vitro in the South Pacific Regional Germplasm Centre at Fiji. The core collection is a valuable resource for food security of the South Pacific region and is currently being utilized by the breeding programmes of small Pacific Island countries to broaden the genetic base of the crop.

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Acknowledgements

These studies were jointly supported by the AusAID Taro Genetic Resources: Conservation and Utilization Project, Australian Centre for International Agricultural Research (ACIAR) Project CS2/94/43, International Plant Genetic Resources Institute (IPGRI) and the Secretariat of the Pacific Community (SPC).

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Author notes

1. D. Singh
   Present address: Plant Breeding Institute Cobbitty, University of Sydney, Sydney, NSW, Australia
2. E. S. Mace
   Present address: Department of Primary Industries and Fisheries, Hermitage Research Station, 604 Yangan Road, Warwick, QLD, Australia

Authors and Affiliations

1. National Agricultural Research Institute, Lae, Papua New Guinea
   D. Singh, T. Okpul & R. Kambuou
2. Secretariat of the Pacific Community, Suva, Fiji
   D. Singh, M. Taylor & D. Hunter
3. School of Land, Crop and Food Sciences, University of Queensland, Brisbane, QLD, Australia
   E. S. Mace & I. D. Godwin
4. International Plant Genetic Resources Institute, Office of South Asia, New Delhi, India
   P. N. Mathur
5. International Plant Genetic Resources Institute, Regional Office for Asia, the Pacific and Oceania, Serdang, Malaysia
   V. Ramanatha Rao
6. Sydney, NSW, 2002, Australia
   G. Jackson

Authors

1. D. Singh
2. E. S. Mace
3. I. D. Godwin
4. P. N. Mathur
5. T. Okpul
6. M. Taylor
7. D. Hunter
8. R. Kambuou
9. V. Ramanatha Rao
10. G. Jackson

Corresponding author

Correspondence toD. Singh.

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Singh, D., Mace, E.S., Godwin, I.D. et al. Assessment and rationalization of genetic diversity of Papua New Guinea taro (Colocasia esculenta) using SSR DNA fingerprinting.Genet Resour Crop Evol 55, 811–822 (2008). https://doi.org/10.1007/s10722-007-9286-6

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• Received: 19 March 2007
• Accepted: 28 September 2007
• Published: 03 November 2007
• Issue date: September 2008
• DOI: https://doi.org/10.1007/s10722-007-9286-6

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