Silk fibroin hydrogel promote burn wound healing through regulating TLN1 expression and affecting cell adhesion and migration (original) (raw)

Abstract

Background

Skin injury is a kind of common tissue damage in daily life and war. Silk fibroin (SF) is becoming an engineered material for skin wound repair due to its superior unique physical and chemical properties. The present study aimed to illustrate mechanism of SF hydrogel promoting skin repair in the second degree burn mice.

Methods

Heat shock models were established. In vitro, cells were culture for 50 min at 44 °C water bath; while in vivo, the skin of anesthetic mice were treat with soldering iron at 90 °C. Then, they divided into silk fibroin gel group, purilon gel group and control (blank) group. The cellular activity of proliferation and apoptosis was detected by Kit-8, flow cytometry and HE-staining, and the migration and adhesion were detected by scratch test. qRT-PCR and WB were employed to detected adhesion and migration related genes and proteins expression. TLN1 siRNA and overexpression technologies were also employed to illustrate the potential mechanism of SF effects.

Results

Compared with the purilon gel group and control group, SF hydrogel could enhance cell proliferation, migration and adhesion and increase the expression of adhesion and migration related proteins (P < 0.05), which promote burn wound healing.

Conclusions

Through the inhibition, overexpression and rescue experiments of Talin1, we proved that silk fibroin hydrogel promote burn wound healing through regulating TLN1 expression and affecting cell adhesion and migration.

The alternative text for this image may have been generated using AI.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

The alternative text for this image may have been generated using AI.

Fig. 2

The alternative text for this image may have been generated using AI.

Fig. 3

The alternative text for this image may have been generated using AI.

Fig. 4

The alternative text for this image may have been generated using AI.

Fig. 5

The alternative text for this image may have been generated using AI.

Similar content being viewed by others

References

  1. Mitrousis N, Fokina A, Shoichet MS. Biomaterials for cell transplantation. Nat Rev Materials. 2018;3:441–56.
    Article CAS Google Scholar
  2. Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, et al. Silk-based biomaterials. Biomaterials. 2003;24:401–16.
    Article CAS Google Scholar
  3. Samal SK, Dash M, Chiellini F, Kaplan DL, Chiellini E. Silk microgels formed by proteolytic enzyme activity. Acta Biomater. 2013;9:8192–9.
    Article CAS Google Scholar
  4. Wang X, Kluge JA, Leisk GG, Kaplan DL. Sonication-induced gelation of silk fibroin for cell encapsulation. Biomaterials. 2008;29:1054–64.
    Article CAS Google Scholar
  5. Zhang YQ. Applications of natural silk protein sericin in biomaterials. Biotechnol Adv. 2003;20:91–100.
    Article Google Scholar
  6. Zuluaga-Velez A, Combita-Merchan DF, Buitrago-Sierra R, Santa JF, Aguilar-Fernandez E, Sepulveda-Arias JC. Silk fibroin hydrogels from the Colombian silkworm Bombyx mori L: evaluation of physicochemical properties. PLoS ONE. 2019;14:e0213303.
    Article Google Scholar
  7. Ma D, An G, Liang M, Liu Y, Zhang B, Wang Y. A composited PEG-silk hydrogel combining with polymeric particles delivering rhBMP-2 for bone regeneration. Mater Sci Eng C Mater Biol Appl. 2016;65:221–31.
    Article CAS Google Scholar
  8. Chouhan D, Lohe TU, Samudrala PK, Mandal BB.In situ forming injectable silk fibroin hydrogel promotes skin regeneration in full thickness burn wounds.Adv Healthcare Mater. 2018;7:e1801092.
    Article Google Scholar
  9. Ribeiro VP, Silva-Correia J, Goncalves C, Pina S, Radhouani H, Montonen T, et al. Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death. PLoS ONE. 2018;13:e0194441.
    Article Google Scholar
  10. Sonnemann KJ, Bement WM. Wound repair: toward understanding and integration of single-cell and multicellular wound responses. Annu Rev Cell Dev Biol. 2011;27:237–63.
    Article CAS Google Scholar
  11. Kang H. Progress on evaluation criterion of wound healing. Chin J Rep Reconstr Surg. 2001;15:126–9.
    Google Scholar
  12. Monkley SJ, Pritchard CA, Critchley DR. Analysis of the Mammalian Talin2 Gene TLN2. Biochem Biophys Res Commun. 2001;286:880–5.
    Article CAS Google Scholar
  13. Debrand E, El Jai Y, Spence L, Bate N, Praekelt U, Pritchard CA, et al. Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms. FEBS J. 2009;276:1610–28.
    Article CAS Google Scholar
  14. Manso AM, Li R, Monkley SJ, Cruz NM, Ong S, Lao DH, et al. Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload. J Biol Chem. 2013;288:4252–64.
    Article CAS Google Scholar
  15. Monkley SJ, Kostourou V, Spence L, Petrich B, Coleman S, Ginsberg MH, et al. Endothelial cell talin1 is essential for embryonic angiogenesis. Dev Biol. 2011;349:494–502.
    Article CAS Google Scholar
  16. Rodius S, Chaloin O, Moes M, Schaffner-Reckinger E, Landrieu I, Lippens G, et al. The talin rod IBS2 alpha-helix interacts with the beta3 integrin cytoplasmic tail membrane-proximal helix by establishing charge complementary salt bridges. J Biol Chem. 2008;283:24212–23.
    Article CAS Google Scholar
  17. Haage A, Goodwin K, Whitewood A, Camp D, Bogutz A, Turner CT, et al. Talin autoinhibition regulates Cell-ECM adhesion dynamics and wound healing in vivo. Cell Rep. 2018;25:e5.
    Article Google Scholar
  18. Fernandes AC, Franca JP, Gaiba S, Aloise AC, Oliveira AF, Moraes AA, et al. Development of experimental in vitro burn model. Acta Cir Bras. 2014;29:15–20.
    Article Google Scholar
  19. Farokhi M, Mottaghitalab F, Fatahi Y, Khademhosseini A, Kaplan DL. Overview of silk fibroin use in wound dressings. Trends Biotechnol. 2018;36:907–22.
    Article CAS Google Scholar
  20. Floren M, Migliaresi C, Motta A. Processing techniques and applications of silk hydrogels in bioengineering. J Funct Biomater. 2016;7:26.
    Article Google Scholar
  21. Wang X, Partlow B, Liu J, Zheng Z, Su B, Wang Y, et al. Injectable silk-polyethylene glycol hydrogels. Acta Biomater. 2015;12:51–61.
    Article Google Scholar
  22. Reinke JM, Sorg H. Wound repair and regeneration. Eur Surg Res. 2012;49:35–43.
    Article CAS Google Scholar
  23. Park YR, Sultan MT, Park HJ, Lee JM, Ju HW, Lee OJ, et al.Corrigendum to “NF-kappaB signaling is key in the wound healing processes of silk fibroin” [Acta Biomater. 67 (2018), 183–195]. Acta biomaterialia. 2018;72:461.
    Article Google Scholar
  24. Hou Q, He WJ, Hao HJ, Han QW, Chen L, Dong L, et al. The four-herb Chinese medicine ANBP enhances wound healing and inhibits scar formation via bidirectional regulation of transformation growth factor pathway. PloS ONE. 2014;9:e112274.
    Article Google Scholar
  25. Hess CT. Checklist for improved wound outcomes (with a focus on pressure ulcers): part 2. Adv Skin Wound Care. 2012;25:192.
    Article Google Scholar

Download references

Acknowledgements

I am grateful to the laboratory of the Basic Medical College and the Second Affiliated Hospital of Harbin Medical University.

Author information

Authors and Affiliations

  1. Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, 150001, Harbin, China
    Ying Guan, Xiaojuan Zhang, Zhibin Peng, Bo jiang, Min Liang & Yansong Wang
  2. Department of Orthopedic Surgery, General Hospital of Heilongjiang Province Land Reclamation Headquarter, 150001, Harbin, China
    Feng Sun

Authors

  1. Ying Guan
  2. Feng Sun
  3. Xiaojuan Zhang
  4. Zhibin Peng
  5. Bo jiang
  6. Min Liang
  7. Yansong Wang

Corresponding author

Correspondence toYansong Wang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Mice handling and care followed the rules of the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Animal experiment design gained approval from the ethical committee of Harbin Medical University.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

About this article

Cite this article

Guan, Y., Sun, F., Zhang, X. et al. Silk fibroin hydrogel promote burn wound healing through regulating TLN1 expression and affecting cell adhesion and migration.J Mater Sci: Mater Med 31, 48 (2020). https://doi.org/10.1007/s10856-020-06384-8

Download citation