Magnetic field induced residual dipolar couplings of imino groups in nucleic acids from measurements at a single magnetic field (original) (raw)

Abstract

For base-paired nucleic acids, variations in 1_J_NH and the imino 1H chemical shift are both dominated by hydrogen bond length. In the absence of molecular alignment, the 1_J_NH coupling for the imino proton then can be approximated by 1_J_NH = (1.21Hz/ppm)δH − 103.5 ± 0.6 Hz, where δH represents the chemical shift of the imino proton in ppm. This relation permits imino residual dipolar couplings (RDCs) resulting from magnetic susceptibility anisotropy (MSA) to be extracted from measurement of (1_J_NH + RDC) splittings at a single magnetic field strength. Magnetic field-induced RDCs were measured for tRNAVal and the alignment tensor determined from magnetic-field alignment of tRNAVal agrees well with the tensor calculated by summation of the MSA tensors of the individual nucleobases.

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Acknowledgement

This work was supported in part by the Intramural Research Program of the NIDDK, NIH, and by the Intramural AIDS-Targeted Antiviral Program of the Office of the Director, NIH, and NIH grant AI33098 (AP); MPL was supported in part by an NIH Training Grant T32 GM65103. We thank Dr. Sam E. Butcher and Dipa Sashital for providing us with the15N-enriched U2-U6 sample.

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Authors and Affiliations

1. Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892, USA
   Jinfa Ying, Alexander Grishaev & Ad Bax
2. Department of Chemistry and Biochemistry, 215 UCB, University of Colorado, Boulder, CO, 80309-0215, USA
   Michael P. Latham & Arthur Pardi

Authors

1. Jinfa Ying
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2. Alexander Grishaev
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3. Michael P. Latham
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4. Arthur Pardi
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Correspondence toArthur Pardi or Ad Bax.

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Jinfa Ying, Alexander Grishaev and Michael P. Latham contributed equally to this work.

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Ying, J., Grishaev, A., Latham, M.P. et al. Magnetic field induced residual dipolar couplings of imino groups in nucleic acids from measurements at a single magnetic field.J Biomol NMR 39, 91–96 (2007). https://doi.org/10.1007/s10858-007-9181-7

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• Received: 22 May 2007
• Revised: 11 July 2007
• Accepted: 17 July 2007
• Published: 07 August 2007
• Issue Date: October 2007
• DOI: https://doi.org/10.1007/s10858-007-9181-7

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