Analysis of the toxicity of gold nano particles on the immune system: effect on dendritic cell functions (original) (raw)

Abstract

The effect of manufactured gold nanoparticles (NPs) on the immune system was analysed through their ability to perturb the functions of dendritic cells (DCs), a major actor of both innate and acquired immune responses. For this purpose, DCs were produced in culture from mouse bone marrow progenitors. The analysis of the viability of the cells after their incubation in the presence of gold NPs shows that these NPs are not cytotoxics even at high concentration. Furthermore, the phenotype of the DC is unchanged after the addition of NPs, indicating that there is no activation of the DC. However, the analysis of the cells at the intracellular level reveals important amounts of gold NPs amassing in endocytic compartments. Furthermore, the secretion of cytokines is significantly modified after such internalisation indicating a potential perturbation of the immune response.

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Acknowledgements

We acknowledge members of CellNanoTox project (FP6-NMP-CT-2006-032731) of the European Community, especially to Dr. Daniele Bonacchi for synthesis and production of the NPs.

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Authors and Affiliations

1. Inserm, U823, Centre de Recherche Albert Bonniot, Grenoble, France
   Christian L. Villiers, Heidi Freitas, Rachel Couderc, Marie-Bernadette Villiers & Patrice N. Marche
2. Université Joseph Fourier, Grenoble, France
   Christian L. Villiers, Heidi Freitas, Rachel Couderc, Marie-Bernadette Villiers & Patrice N. Marche

Authors

1. Christian L. Villiers
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2. Heidi Freitas
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3. Rachel Couderc
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4. Marie-Bernadette Villiers
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5. Patrice N. Marche
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Correspondence toChristian L. Villiers.

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Villiers, C.L., Freitas, H., Couderc, R. et al. Analysis of the toxicity of gold nano particles on the immune system: effect on dendritic cell functions.J Nanopart Res 12, 55–60 (2010). https://doi.org/10.1007/s11051-009-9692-0

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• Received: 05 June 2009
• Accepted: 24 June 2009
• Published: 10 July 2009
• Issue Date: January 2010
• DOI: https://doi.org/10.1007/s11051-009-9692-0

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