Selection of suitable reference genes for expression analysis in human glioma using RT-qPCR (original) (raw)

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References

1. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW, Kleihues P (2007) The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol 114:97–109. doi:10.1007/s00401-007-0243-4
   Article PubMed Central PubMed Google Scholar
2. Appin CL, Brat DJ (2014) Molecular genetics of gliomas. Cancer J 20:66–72. doi:10.1097/PPO.0000000000000020
   Article CAS PubMed Google Scholar
3. Yin AA, Cheng JX, Zhang X, Liu BL (2013) The treatment of glioblastomas: a systematic update on clinical Phase III trials. Crit Rev Oncol Hematol 87:265–282. doi:10.1016/j.critrevonc.2013.01.007
   Article PubMed Google Scholar
4. Sami A, Karsy M (2013) Targeting the PI3K/AKT/mTOR signaling pathway in glioblastoma: novel therapeutic agents and advances in understanding. Tumour Biol 34:1991–2002. doi:10.1007/s13277-013-0800-5
   Article CAS PubMed Google Scholar
5. Rinne ML, Lee EQ, Nayak L, Norden AD, Beroukhim R, Wen PY, Reardon DA (2013) Update on bevacizumab and other angiogenesis inhibitors for brain cancer. Expert Opin Emerg Drugs 18:137–153. doi:10.1517/14728214.2013.794784
   Article CAS PubMed Google Scholar
6. Reardon DA, Wucherpfennig KW, Freeman G, Wu CJ, Chiocca EA, Wen PY, Curry WT Jr, Mitchell DA, Fecci PE, Sampson JH, Dranoff G (2013) An update on vaccine therapy and other immunotherapeutic approaches for glioblastoma. Expert Rev Vaccines 12:597–615. doi:10.1586/erv.13.41
   Article PubMed Central CAS PubMed Google Scholar
7. Akiyama Y, Komiyama M, Miyata H, Yagoto M, Ashizawa T, Iizuka A, Oshita C, Kume A, Nogami M, Ito I, Watanabe R, Sugino T, Mitsuya K, Hayashi N, Nakasu Y, Yamaguchi K (2014) Novel cancer-testis antigen expression on glioma cell lines derived from high-grade glioma patients. Oncol Rep 31:1683–1690. doi:10.3892/or.2014.3049
   PubMed Google Scholar
8. Wang X, Sheng P, Guo X, Wang J, Hou L, Hu G, Luo C, Dong Y, Lu Y (2013) Identification and expression of a novel MDM4 splice variant in human glioma. Brain Res 1537:260–266. doi:10.1016/j.brainres.2013.07.054
   Article CAS PubMed Google Scholar
9. de Tayrac M, Aubry M, Saikali S, Etcheverry A, Surbled C, Guenot F, Galibert MD, Hamlat A, Lesimple T, Quillien V, Menei P, Mosser J (2011) A 4-gene signature associated with clinical outcome in high-grade gliomas. Clin Cancer Res 17:317–327. doi:10.1158/1078-0432.CCR-10-1126
   Article PubMed Google Scholar
10. Nolan T, Hands RE, Bustin SA (2006) Quantification of mRNA using real-time RT-PCR. Nat Protoc 1:1559–1582. doi:10.1038/nprot.2006.236
    Article CAS PubMed Google Scholar
11. Wong ML, Medrano JF (2005) Real-time PCR for mRNA quantitation. Biotechniques 39:75–85
    Article CAS PubMed Google Scholar
12. Dundas J, Ling M (2012) Reference genes for measuring mRNA expression. Theory Biosci 131:215–223. doi:10.1007/s12064-012-0152-5
    Article CAS PubMed Google Scholar
13. Kreth S, Heyn J, Grau S, Kretzschmar HA, Egensperger R, Kreth FW (2010) Identification of valid endogenous control genes for determining gene expression in human glioma. Neuro Oncol 12:570–579. doi:10.1093/neuonc/nop072
    Article PubMed Central CAS PubMed Google Scholar
14. Gresner SM, Golanska E, Kulczycka-Wojdala D, Jaskolski DJ, Papierz W, Liberski PP (2011) Selection of reference genes for gene expression studies in astrocytomas. Anal Biochem 408:163–165. doi:10.1016/j.ab.2010.09.010
    Article CAS PubMed Google Scholar
15. Valente V, Teixeira SA, Neder L, Okamoto OK, Oba-Shinjo SM, Marie SK, Scrideli CA, Paco-Larson ML, Carlotti CG Jr (2009) Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR. BMC Mol Biol 10:17. doi:10.1186/1471-2199-10-17
    Article PubMed Central PubMed Google Scholar
16. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45
    Article PubMed Central CAS PubMed Google Scholar
17. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3(7):RESEARCH0034
    Article PubMed Central PubMed Google Scholar
18. Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64:5245–5250. doi:10.1158/0008-5472.CAN-04-0496
    Article CAS PubMed Google Scholar
19. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: bestKeeper–Excel-based tool using pair-wise correlations. Biotechnol Lett 26:509–515
    Article CAS PubMed Google Scholar
20. Zhong H, Simons JW (1999) Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia. Biochem Biophys Res Commun 259:523–526. doi:10.1006/bbrc.1999.0815
    Article CAS PubMed Google Scholar
21. Glare EM, Divjak M, Bailey MJ, Walters EH (2002) beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels. Thorax 57:765–770
    Article PubMed Central CAS PubMed Google Scholar
22. Tricarico C, Pinzani P, Bianchi S, Paglierani M, Distante V, Pazzagli M, Bustin SA, Orlando C (2002) Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem 309:293–300
    Article CAS PubMed Google Scholar
23. de Kok JB, Roelofs RW, Giesendorf BA, Pennings JL, Waas ET, Feuth T, Swinkels DW, Span PN (2005) Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genes. Lab Invest 85:154–159. doi:10.1038/labinvest.3700208
    Article PubMed Google Scholar
24. Ohl F, Jung M, Xu C, Stephan C, Rabien A, Burkhardt M, Nitsche A, Kristiansen G, Loening SA, Radonic A, Jung K (2005) Gene expression studies in prostate cancer tissue: which reference gene should be selected for normalization? J Mol Med (Berl) 83:1014–1024. doi:10.1007/s00109-005-0703-z
    Article CAS Google Scholar
25. de Jonge HJ, Fehrmann RS, de Bont ES, Hofstra RM, Gerbens F, Kamps WA, de Vries EG, van der Zee AG, te Meerman GJ, ter Elst A (2007) Evidence based selection of housekeeping genes. PLoS ONE 2:e898. doi:10.1371/journal.pone.0000898
    Article PubMed Central PubMed Google Scholar

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