Identification and prevalence investigation of cyathocotylid trematode (Trematoda: Digenea: Cyathocotylidae) in Carassius auratus in Wuhu, China (original) (raw)

Abstract

The family Cyathocotylidae trematode is a world-widely distributed parasite whose adults are mainly found in fish, reptiles, birds, and mammals in both freshwater and marine environments. However, little is known of the prevalence of these trematode in China. For the first time, we found Cyathocotylidae trematode in the Wuhu area. Therefore, we investigated the prevalence of metacercariae in Carassius auratus from Zhang Lake, Kui Lake, Qingyi River, and Yangtze River (Wuhu Section) in Wuhu area. A total of 392 one-year-old C. auratus were tested in January, April, July, and October 2019, respectively. After the fish were euthanized, the back, chest, and tail muscles were used for preliminary screening for the presence of metacercariae by direct compression method. Metacercariae were isolated by the artificial digestion method and then morphologically and molecularly identified by _cox_1 (642 bp) and _ITS_2 (418 bp) sequences amplification by PCR. Based on morphological features and sequence analysis, they were identified as cyathocotylid metacercariae. The four water sources have different degrees of prevalence throughout the year, the prevalence ranges from 28.45% (29/102) in Yangtze River to 50.55% (46/91) in Zhang Lake, with an average prevalence of 40.56% (159/392). There were also differences in the prevalence in different months: The highest occurred in July, which was 81.63% (80/98), while the lowest occurred in January, which was 8.89% (8/90). This study can provide basic data for the prevention and control of cyathocotylid trematode in this area.

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Data availability

The _cox_1 and _ITS_2 data presented in this study can be found in GenBank under accession number ON411423 – ON411427 and ON261172 – ON261176.

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Acknowledgements

The authors gratefully acknowledge Prof. Chaopin Li (Wannan Medical College) for his help with morphological identification. The authors also are gratefully acknowledge the Central Laboratory of Wannan Medical College for providing experimental facilities and conditions.

Funding

This work was supported by the Key Projects of Natural Science Research of Universities in Anhui Province (grant no. KJ2019A0403).

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Authors and Affiliations

1. Department of Medical Parasitology, Wannan Medical College, 241002, Wuhu, Anhui, China
   Feng Jiang, Wanyu Zhang, Yang Pan, Xudong Yu, Jiacheng Cao, Ningning Du, Mengyi Zhu, Shengli Gu & Xiaodong Zhan
2. Anhui Provincial Key Laboratory of Biological Macro-molecules, 241002, Wuhu, Anhui, China
   Feng Jiang & Xiaodong Zhan

Authors

1. Feng Jiang
2. Wanyu Zhang
3. Yang Pan
4. Xudong Yu
5. Jiacheng Cao
6. Ningning Du
7. Mengyi Zhu
8. Shengli Gu
9. Xiaodong Zhan

Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by FJ, WZ, YP, XY, JC, ND, MZ, and SG. The first draft of the manuscript was written by FJ and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence toXiaodong Zhan.

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All procedures performed in studies involving animals were in accordance with the ethical standards approved by the Wannan Medical College Animal Experiments Local Ethics Committee (Approval number: LLSC-2018-004).

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Jiang, F., Zhang, W., Pan, Y. et al. Identification and prevalence investigation of cyathocotylid trematode (Trematoda: Digenea: Cyathocotylidae) in Carassius auratus in Wuhu, China.Vet Res Commun 47, 1185–1193 (2023). https://doi.org/10.1007/s11259-022-10049-y

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• Received: 30 September 2022
• Accepted: 05 December 2022
• Published: 17 January 2023
• Version of record: 17 January 2023
• Issue date: September 2023
• DOI: https://doi.org/10.1007/s11259-022-10049-y

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