Best practices for authenticating cell lines (original) (raw)

Abstract

Experiments using cell cultures are only valid to the extent that the cell culture is a true model system for the biological system being investigated. To assure that a cell line is and remains an appropriate biological model, its identity, purity, ploidy, and phenotype must be maintained. These characteristics comprise and determine the authenticity of a cell line. Routine monitoring of the cell line through microscopic examination of morphology can help to determine authenticity, as can the determination of phenotypic status. Assays designed to confirm cell identity and ploidy and freedom from cross-contaminating cell types may need to be performed at certain times, as such information may not be obtained through morphologic and phenotypic examinations alone. The best practices associated with establishing cell line authenticity are described in this article.

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Acknowledgements

The authors acknowledge the input and expert review of the following members of the Best Practices in Cell Culture Workgroup: John M. Baust, Gertrude Case Buehring, Lia H. Campbell, Eugene Elmore, John W. Harbell, Paul Price, and Frank Simione.

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Authors and Affiliations

1. RMC Pharmaceutical Solutions, Inc., 1851 Lefthand Circle, Suite A, Longmont, CO, 80501, USA
   Raymond W. Nims
2. ATCC, 10801 University Blvd., Manassas, VA, 20169, USA
   Yvonne Reid

Authors

1. Raymond W. Nims
2. Yvonne Reid

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Correspondence toRaymond W. Nims.

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Editor: Tetsuji Okamoto

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Nims, R.W., Reid, Y. Best practices for authenticating cell lines.In Vitro Cell.Dev.Biol.-Animal 53, 880–887 (2017). https://doi.org/10.1007/s11626-017-0212-8

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• Received: 31 July 2017
• Accepted: 17 October 2017
• Published: 01 December 2017
• Issue date: December 2017
• DOI: https://doi.org/10.1007/s11626-017-0212-8

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