Preliminary analysis of the cauliflower mitochondrial proteome (original) (raw)

Abstract

Purified cauliflower (Brassica oleracea var. botrytis) mitochondrial proteins fractionated into soluble, membrane, integral membrane and peripheral membrane samples were analyzed by 2D- PAGE (isoelectric focusing/ SDS polyacrylamide gel electrophoresis). 2D gels patterns were compared using the Imager Master 2D Elite software. 561 silver stained protein spots were resolved after electrophoresis under standard conditions of a whole protein extract. In the soluble fraction a prevalent number of more intense protein spots was observed. The cauliflower protein 2D patterns resembled Arabidopsis thaliana 2D patterns. The two protein spots selected which occupied a similar isoelectric point positions on both gels represented the same proteins as revealed by ESI-MS analysis of cauliflower proteins. The third selected spot belongs to unidentified proteins. The comparative analysis of mitochondrial suborganellar fractions proved the usefulness of this approach.

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Authors and Affiliations

1. Institute of Dendrology of the Polish Academy of Sciences, 5 Parkowa Str., 62-035, Kórnik, Poland
   T. Pawlowski
2. Department of Plant Molecular Biology, Institute of Molecular Biology and Biotechnology, A. Mickiewicz University, 5 Mi dzychodzka Str., 60-371, Pozna, Poland
   M. Rurek, S. Janicka, K. D. Raczynska & H. Augustyniak

Authors

1. T. Pawlowski
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2. M. Rurek
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3. S. Janicka
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4. K. D. Raczynska
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5. H. Augustyniak
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Correspondence toH. Augustyniak.

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Pawlowski, T., Rurek, M., Janicka, S. et al. Preliminary analysis of the cauliflower mitochondrial proteome.Acta Physiol Plant 27, 275–281 (2005). https://doi.org/10.1007/s11738-005-0003-9

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• Received: 02 April 2004
• Accepted: 01 July 2004
• Issue Date: September 2005
• DOI: https://doi.org/10.1007/s11738-005-0003-9

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