Molecular typing of fecal eukaryotic microbiota of human infants and their respective mothers (original) (raw)

Abstract

The micro-eukaryotic diversity from the human gut was investigated using universal primers directed towards 18S rRNA gene, fecal samples being the source of DNA. The subjects in this study included two breast-fed and two formula-milk-fed infants and their mothers. The study revealed that the infants did not seem to harbour any micro-eukaryotes in their gut. In contrast, there were distinct eukaryotic microbiota present in the mothers. The investigation is the first of its kind in the comparative study of the human feces to reveal the presence of micro-eukaryotic diversity variance in infants and adults from the Indian subcontinent. The micro-eukaryotes encountered during the investigation include known gut colonizers like Blastocystis and some fungi species. Some of these micro-eukaryotes have been speculated to be involved in clinical manifestations of various diseases. The study is an attempt to highlight the importance of micro-eukaryotes in the human gut.

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Authors and Affiliations

1. Insect Molecular Biology Unit, National Centre for Cell Science, Pune University campus, Ganeshkhind, Pune, 411 007, Maharashtra, India
   Prashant K Pandey, Jay Siddharth, Pankaj Verma, Milind S Patole & Yogesh S Shouche
2. Gastroenterology Unit, Department of pediatrics, KEM Hospital, Rasta Peth, Pune, 411 011, India
   Ashish Bavdekar

Authors

1. Prashant K Pandey
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2. Jay Siddharth
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3. Pankaj Verma
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4. Ashish Bavdekar
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5. Milind S Patole
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6. Yogesh S Shouche
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Corresponding author

Correspondence toYogesh S Shouche.

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Corresponding editor: Anand Kumar Bachhawat

[Pandey PK, Siddharth J, Verma P, Bavdekar A, Patole MS and Shouche YS 2012 Molecular typing of fecal eukaryotic microbiota of human infants and their respective mothers. J. Biosci. 37 1–6] DOI

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Pandey, P.K., Siddharth, J., Verma, P. et al. Molecular typing of fecal eukaryotic microbiota of human infants and their respective mothers.J Biosci 37, 221–226 (2012). https://doi.org/10.1007/s12038-012-9197-3

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• Received: 12 December 2011
• Accepted: 19 March 2012
• Published: 19 April 2012
• Issue Date: June 2012
• DOI: https://doi.org/10.1007/s12038-012-9197-3

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