Capillary Electrophoresis Method Validation for Organic Acids Assessment in Probiotics (original) (raw)

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Abstract

Quantitative evaluation of organic acids in plants and foodstuff is important for flavour and nutritional studies and also could be used as indicator of bacterial activity. Organic acids occurring in foods are additives or end-products of carbohydrate metabolism of lactic acid bacteria (LAB). A good selection of LAB (and a criterion should be the content in organic acids) allows the control of mould growth and improves the shelf life of many fermented products and, therefore, reduces health risks due to exposure to mycotoxins. A simple, rapid (15 min) and reliable capillary electrophoresis method was partially validated for the simultaneous quantification of nine aliphatic (formic, oxalic, succinic, malic, tartaric, acetic, citric, lactic, butyric) and three aromatic (benzoic, phenyllactic and hydroxyphenyllactic) organic acids in the fermentation products of lactic acid bacteria. The method was validated in terms of linearity of response, limit of detection (LoD), limit of quantification (LoQ), precisions (i.e. intra-day, inter-day reproducibility) and recovery. The method is simple, rapid, reliable and cost-effective.

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Acknowledgments

This research was supported by the National Centre for Program Management PN-II-PT-PCCA-2011-3.1-0969/2012 and Nucleu-Biodiv grant PN-09-360106/2009, coordinated by Romanian Agency for Scientific Research.

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Conflict of Interest

All the authors declare that they have no conflict of interest. All the funds are provided by Romanian Agency for Scientific Research.

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

1. National Institute for Biological Science, Splaiul Independentei 296, 060031, Bucharest, Romania
   Florentina Gatea, Eugenia D. Teodor, Gabriela Paun & Alina O. Matei
2. Department of Analytical Chemistry and Environmental Engineering, Politehnica University of Bucharest, 1-7 Polizu Street, 011061, Bucharest, Romania
   Gabriel L. Radu

Authors

1. Florentina Gatea
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2. Eugenia D. Teodor
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3. Gabriela Paun
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4. Alina O. Matei
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5. Gabriel L. Radu
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Corresponding author

Correspondence toEugenia D. Teodor.

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Gatea, F., Teodor, E.D., Paun, G. et al. Capillary Electrophoresis Method Validation for Organic Acids Assessment in Probiotics.Food Anal. Methods 8, 1335–1340 (2015). https://doi.org/10.1007/s12161-014-0018-1

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• Received: 04 July 2014
• Accepted: 07 October 2014
• Published: 14 October 2014
• Issue Date: May 2015
• DOI: https://doi.org/10.1007/s12161-014-0018-1

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