Utilisation of steviol glycosides from Stevia rebaudiana (Bertoni) by lactobacilli and bifidobacteria in in vitro conditions (original) (raw)

Abstract

In the current study, eight strains of bifidobacteria and seven strains of lactobacilli were tested for their ability to grow in the presence of rebaudioside A and steviol glycosides from the sweetener Natusweet M001 originating from herb Stevia rebaudiana (Bertoni). Stevia is gaining popularity as a natural, non-caloric sugar substitute, and recently, it was allowed as a food additive by European Union too. Utilisation of steviol glycosides by intestinal microbiota suggests that they might have potential prebiotic effect. Based on the evaluation of bacterial density and pH values in our in vitro study, it was found that lactobacilli and bifidobacteria tested were able to utilise steviol glycosides as a carbon source only to a very limited extent. All strains tested showed significantly lower change in the absorbance A540 (P < 0.05) and pH decrease of the growth media as compared with the positive controls (medium containing glucose as a carbon source and de Man Rogosa Sharpe broth). We concluded that a suggested prebiotic effect was not confirmed either in the case of rebaudioside A or in the case of the sweetener Natusweet M001 containing a mixture of steviol glycosides.

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Acknowledgments

This study was supported by Ministry of Agriculture of the Czech Republic, NAZV, project nos. QI91B274 and QJ1210093, and by Institutional support no. RO0513. The authors are thankful to Mr. Mario Megela for the technical assistance.

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Authors and Affiliations

1. Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 16521, Prague, Czech Republic
   Gabriela Kunová & Vojtěch Rada
2. Dairy Research Institute, Ltd., Ke Dvoru 12a, 16000, Prague 6, Czech Republic
   Gabriela Kunová, Adrien Vidaillac & Ivana Lisova

Authors

1. Gabriela Kunová
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2. Vojtěch Rada
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3. Adrien Vidaillac
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4. Ivana Lisova
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Correspondence toGabriela Kunová.

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Kunová, G., Rada, V., Vidaillac, A. et al. Utilisation of steviol glycosides from Stevia rebaudiana (Bertoni) by lactobacilli and bifidobacteria in in vitro conditions.Folia Microbiol 59, 251–255 (2014). https://doi.org/10.1007/s12223-013-0291-1

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• Received: 20 March 2013
• Accepted: 30 October 2013
• Published: 19 November 2013
• Issue Date: May 2014
• DOI: https://doi.org/10.1007/s12223-013-0291-1

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