Chemisch definiert – ein zellbasierter Zytotoxizitätsassay ohne fötales Kälberserum (original) (raw)
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Abstract
Tests for cytotoxicity–respectively biocompatibility–are performed e. g. for biological evaluation of medical devices or during the development of microphysiometric systems. Here, often cell culture methods which involve the use of fetal bovine serum (FBS) are employed. However, using FBS raises scientific and ethical concerns. In the presented work a protocol for a chemically defined test for cytotoxicity is described.
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Literatur
- van der Valk J, Brunner D, De Smet K et al. (2010) Optimization of chemically defined cell culture media–replacing fetal bovine serum in mammalian in vitro methods. Toxicol In Vitro 24:1053–1063
Article PubMed Google Scholar - van der Valk J et al. (2017) Fetal bovine serum (FBS): past–present–future. (Veröffentlichung in Vorbereitung)
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Authors and Affiliations
- cellasys GmbH - R&D, Ohmstraße 8, D-80802, München, Deutschland
Joachim Wiest
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Correspondence toJoachim Wiest.
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Wiest, J. Chemisch definiert – ein zellbasierter Zytotoxizitätsassay ohne fötales Kälberserum.Biospektrum 23, 61–62 (2017). https://doi.org/10.1007/s12268-017-0768-6
- Published: 05 February 2017
- Issue date: February 2017
- DOI: https://doi.org/10.1007/s12268-017-0768-6