Long non-coding RNA BANCR regulates growth and metastasis and is associated with poor prognosis in retinoblastoma (original) (raw)

Abstract

Recent evidence shows that BRAF-activated non-coding RNA (BANCR) acts as a critical role in the proliferation and metastasis in malignant melanoma and lung cancer; however, little is known about the significance of lncRNA BANCR in retinoblastoma. The purpose of our study is to explore the role of lncRNA BANCR in retinoblastoma clinical samples and cell lines. The expression of lncRNA BANCR was measured in 60 retinoblastoma samples and normal retina samples by using RT-PCR. The effects of lncRNA BANCR on cell proliferation, migration, and invasion were also explored. In our results, lncRNA BANCR is overexpressed in retinoblastoma tissues and cell lines and is associated with tumor size, choroidal invasion, and optic nerve invasion. Moreover, patients with high levels of lncRNA BANCR expression had poorer survival than those with lower levels of lncRNA BANCR expression. Multivariate analysis showed that increased lncRNA BANCR expression was a poor independent prognostic factor for retinoblastoma patients. Furthermore, knocking down lncRNA BANCR expression significantly suppressed the retinoblastoma cell proliferation, migration, and invasion in vitro. In conclusion, lncRNA BANCR plays a significant role in retinoblastoma aggressiveness and prognosis and may act as a promising target for therapeutic strategy and prognostic prediction.

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References

  1. Villegas VM, Hess DJ, Wildner A, Gold AS, Murray TG. Retinoblastoma. Curr Opin Ophthalmol. 2013;24:581–8.
    Article PubMed Google Scholar
  2. Abramson DH. Retinoblastoma in the 20th century: past success and future challenges the Weisenfeld lecture. Invest Ophthalmol Vis Sci. 2005;46:2683–91.
    Article PubMed Google Scholar
  3. Melamud A, Palekar R, Singh A. Retinoblastoma. Am Fam Physician. 2006;73:1039–44.
    PubMed Google Scholar
  4. Shields CL, Shields JA. Retinoblastoma management: advances in enucleation, intravenous chemoreduction, and intra-arterial chemotherapy. Curr Opin Ophthalmol. 2010;21:203–12.
    Article PubMed Google Scholar
  5. Varan A, Kiratli H, Aydin B, Tarlan B, Poyraz CB, Akyuz C, et al. The treatment of retinoblastoma with four-drug regimen including cisplatin, etoposide, vincristine, and cyclophosphamide. Pediatr Hematol Oncol. 2012;29:529–37.
    Article CAS PubMed Google Scholar
  6. Chawla B, Jain A, Azad R. Conservative treatment modalities in retinoblastoma. Indian J Ophthalmol. 2013;61:479–85.
    Article PubMed PubMed Central Google Scholar
  7. Bowman RJ, Mafwiri M, Luthert P, Luande J, Wood M. Outcome of retinoblastoma in east Africa. Pediatr Blood Cancer. 2008;50:160–2.
    Article CAS PubMed Google Scholar
  8. Gatta G, Capocaccia R, Stiller C, Kaatsch P, Berrino F, Terenziani M. Childhood cancer survival trends in Europe: a Eurocare working group study. J Clin Oncol Off J Am Soc Clin Oncol. 2005;23:3742–51.
    Article Google Scholar
  9. Lin P, O’Brien JM. Frontiers in the management of retinoblastoma. Am J Ophthalmol. 2009;148:192–8.
    Article CAS PubMed Google Scholar
  10. Mercer TR, Dinger ME, Mattick JS. Long non-coding RNAs: insights into functions. Nat Rev Genet. 2009;10:155–9.
    Article CAS PubMed Google Scholar
  11. Tsai MC, Spitale RC, Chang HY. Long intergenic noncoding RNAs: new links in cancer progression. Cancer Res. 2011;71:3–7.
    Article CAS PubMed PubMed Central Google Scholar
  12. Wapinski O, Chang HY. Long noncoding RNAs and human disease. Trends Cell Biol. 2011;21:354–61.
    Article CAS PubMed Google Scholar
  13. Spizzo R, Almeida MI, Colombatti A, Calin GA. Long non-coding RNAs and cancer: a new frontier of translational research? Oncogene. 2012;31:4577–87.
    Article CAS PubMed PubMed Central Google Scholar
  14. Flockhart RJ, Webster DE, Qu K, Mascarenhas N, Kovalski J, Kretz M, et al. BRAFV600E remodels the melanocyte transcriptome and induces BANCR to regulate melanoma cell migration. Genome Res. 2012;22:1006–14.
    Article CAS PubMed PubMed Central Google Scholar
  15. Sun M, Liu XH, Wang KM, Nie FQ, Kong R, Yang JS, et al. Downregulation of BRAF activated non-coding RNA is associated with poor prognosis for non-small cell lung cancer and promotes metastasis by affecting epithelial-mesenchymal transition. Mol Cancer. 2014;13:68.
    Article PubMed PubMed Central Google Scholar
  16. Guo Q, Zhao Y, Chen J, Hu J, Wang S, Zhang D, et al. BRAF-activated long non-coding RNA contributes to colorectal cancer migration by inducing epithelial-mesenchymal transition. Oncol Lett. 2014;8:869–75.
    CAS PubMed PubMed Central Google Scholar
  17. Motoyama K, Inoue H, Nakamura Y, Uetake H, Sugihara K, Mori M. Clinical significance of high mobility group A2 in human gastric cancer and its relationship to let-7 microRNA family. Clin Cancer Res Off J Am Assoc Cancer Res. 2008;14:2334–40.
    Article CAS Google Scholar
  18. Mattick JS, Makunin IV. Non-coding RNA. Hum Mol Genet. 2006;15(1):R17–29.
    Article CAS PubMed Google Scholar
  19. Martin J, Bryar P, Mets M, Weinstein J, Jones A, Martin A, et al. Differentially expressed miRNAs in retinoblastoma. Gene. 2013;512:294–9.
    Article CAS PubMed Google Scholar
  20. Wang J, Wang X, Li Z, Liu H, Teng Y. Microrna-183 suppresses retinoblastoma cell growth, invasion and migration by targeting LRP6. FEBS J. 2014;281:1355–65.
    Article CAS PubMed Google Scholar
  21. Lei Q, Shen F, Wu J, Zhang W, Wang J, Zhang L. MiR-101, downregulated in retinoblastoma, functions as a tumor suppressor in human retinoblastoma cells by targeting EZH2. Oncol Rep. 2014;32:261–9.
    CAS PubMed Google Scholar
  22. Li R, Zhang L, Jia L, Duan Y, Li Y, Bao L, et al. Long non-coding RNA BANCR promotes proliferation in malignant melanoma by regulating MAPK pathway activation. PLoS One. 2014;9, e100893.
    Article PubMed PubMed Central Google Scholar
  23. Wang Y, Guo Q, Zhao Y, Chen J, Wang S, Hu J, et al. BRAF-activated long non-coding RNA contributes to cell proliferation and activates autophagy in papillary thyroid carcinoma. Oncol Lett. 2014;8:1947–52.
    PubMed PubMed Central Google Scholar
  24. Jiang W, Zhang D, Xu B, Wu Z, Liu S, Zhang L, et al. Long non-coding RNA BANCR promotes proliferation and migration of lung carcinoma via MAPK pathways. Biomed Pharmacother. 2015;69:90–5.
    Article CAS PubMed Google Scholar

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Authors and Affiliations

  1. Department of Ophthalmology and Otorhinolaryngology, The People’s Hospital of Zhangqiu City Shandong Province, Zhangqiu, 250200, Shandong, China
    Shizheng Su, Jian Gao, Tao Wang, Ju Wang & Hong Li
  2. Department of Ophthalmology, Jinan Central Hospital Affiliated to Shandong University, No. 105 Jiefang Road, Jinan, 250013, Shandong, China
    Zhen Wang

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  1. Shizheng Su
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  2. Jian Gao
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  3. Tao Wang
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  4. Ju Wang
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  5. Hong Li
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  6. Zhen Wang
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Correspondence toZhen Wang.

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Su, S., Gao, J., Wang, T. et al. Long non-coding RNA BANCR regulates growth and metastasis and is associated with poor prognosis in retinoblastoma.Tumor Biol. 36, 7205–7211 (2015). https://doi.org/10.1007/s13277-015-3413-3

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