Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection (original) (raw)

• Robert-Gangneux F, Dardé M-L. Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev. 2012;25:264–96.
  Article PubMed PubMed Central CAS Google Scholar
• Shiadeh MN, Niyyati M, Fallahi S, Rostami A. Human parasitic protozoan infection to infertility: a systematic review. Parasitol Res. 2016;115:469–77.
  Article PubMed Google Scholar
• Rostami A, Riahi SM, Fakhri Y, Saber V, Hanifehpour H, Valizadeh S, Gholizadeh M, Pouya RH, Gamble HR. The global seroprevalence of Toxoplasma gondii among wild boars: a systematic review and meta-analysis. Vet Parasitol. 2017;244:12–20.
  Article PubMed Google Scholar
• Siyadatpanah A, Tabatabaei F, Zeydi AE, Spotin A, Fallah-Omrani V, Assadi M, Moradi S, Rostami A, Memari F, Hajialiani F. Parasitic contamination of raw vegetables in Amol, North of Iran. Arch Clin Infect Dis. 2013;8:e15983.
  Article Google Scholar
• Shiadeh MN, Rostami A, Pearce B, Gholipourmalekabadi M, Newport D, Danesh M, Mehravar S, Seyyedtabaei S. The correlation between Toxoplasma gondii infection and prenatal depression in pregnant women. Eur J Clin Microbiol Infect Dis. 2016;35:1829–35.
  Article Google Scholar
• Rostami A, Keshavarz H, Shojaee S, Mohebali M, Meamar AR. Frequency of Toxoplasma gondii in HIV positive patients from west of Iran by ELISA and PCR. Iran J Parasitol. 2014;9:474.
  PubMed PubMed Central Google Scholar
• Rostami A, Seyyedtabaei S, Aghamolaie S, Behniafar H, Lasjerdi Z, Abdolrasouli A, Mehravar S, Alvarado-esquivel C. Seroprevalence and risk factors associated with Toxoplasma gondii infection among rural communities in northern IRAN. Revista do Instituto de Medicina Tropical de São Paulo. 2016;58:70.
  Article PubMed PubMed Central Google Scholar
• Shiadeh MN, Moghadam ZB, Adam I, Saber V, Bagheri M, Rostami A. Human infectious diseases and risk of preeclampsia: an updated review of the literature. Infection. 2017;45:589–600.
  Article Google Scholar
• Fallahi S, Rostami A, Shiadeh MN, Behniafar H, Paktinat S. A literature review on maternal–fetal and reproductive disorders of Toxoplasma gondii infection. J Gynecol Obstet Hum Reprod. 2017;. https://doi.org/10.1016/j.jogoh.2017.12.003.
  Article PubMed Google Scholar
• Fallahi S, Rostami A, Birjandi M, Zebardast N, Kheirandish F, Spotin A. Parkinson’s disease and Toxoplasma gondii infection: sero-molecular assess the possible link among patients. Acta Trop. 2017;173:97–101.
  Article PubMed Google Scholar
• Pomares C, Montoya JG. Laboratory diagnosis of congenital toxoplasmosis. J Clin Microbiol. 2016;54:2448–54.
  Article PubMed PubMed Central CAS Google Scholar
• Liu Q, Wang Z-D, Huang S-Y, Zhu X-Q. Diagnosis of toxoplasmosis and typing of Toxoplasma gondii. Parasites Vectors. 2015;8:1.
  Article CAS Google Scholar
• Montoya JG. Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J Infect Dis. 2002;185:S73–82.
  Article PubMed Google Scholar
• Zhang K, Lin G, Han Y, Li J. Serological diagnosis of toxoplasmosis and standardization. Clin Chim Acta. 2016;461:83–9.
  Article PubMed CAS Google Scholar
• Remington JS, Thulliez P, Montoya JG. Recent developments for diagnosis of toxoplasmosis. J Clin Microbiol. 2004;42:941–5.
  Article PubMed PubMed Central Google Scholar
• Petersen E, Borobio MV, Guy E, Liesenfeld O, Meroni V, Naessens A, Spranzi E, Thulliez P. European multicenter study of the LIAISON automated diagnostic system for determination of _Toxoplasma gondii_-specific immunoglobulin G (IgG) and IgM and the IgG avidity index. J Clin Microbiol. 2005;43:1570–4.
  Article PubMed PubMed Central CAS Google Scholar
• Gharavi M, Oormazdi H, Roointan E. A comparative study on sensitivity and specificity of conventional and unconventional IgG and IgM assays for diagnosis of toxoplasmosis. Iran J Public Health. 2008;37:42–5.
  Google Scholar
• Peng D, Hu S, Hua Y, Xiao Y, Li Z, Wang X, Bi D. Comparison of a new gold-immunochromatographic assay for the detection of antibodies against avian influenza virus with hemagglutination inhibition and agar gel immunodiffusion assays. Vet Immunol Immunopathol. 2007;117:17–25.
  Article PubMed CAS Google Scholar
• Wang Y-H, Li X-R, Wang G-X, Yin H, Cai X-P, Fu B-Q, Zhang D-L. Development of an immunochromatographic strip for the rapid detection of Toxoplasma gondii circulating antigens. Parasitol Int. 2011;60:105–7.
  Article PubMed CAS Google Scholar
• Desmonts G, Naot Y, Remington J. Immunoglobulin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired Toxoplasma infections. J Clin Microbiol. 1981;14:486–91.
  PubMed PubMed Central CAS Google Scholar
• Remington JS, Eimstad WM, Araujo FG. Detection of immunoglobulin M antibodies with antigen-tagged latex particles in an immunosorbent assay. J Clin Microbiol. 1983;17:939–41.
  PubMed PubMed Central CAS Google Scholar
• Ferra B, Holec-Gąsior L, Kur J. Serodiagnosis of Toxoplasma gondii infection in farm animals (horses, swine, and sheep) by enzyme-linked immunosorbent assay using chimeric antigens. Parasitol Int. 2015;64:288–94.
  Article PubMed CAS Google Scholar
• Holec-Gąsior L. Toxoplasma gondii recombinant antigens as tools for serodiagnosis of human toxoplasmosis—the current status of studies. Clin Vaccine Immunol. 2013;20(9):1343–51.
  Article PubMed PubMed Central CAS Google Scholar
• Liesenfeld O, Press C, Montoya JG, Gill R, Isaac-Renton JL, Hedman K, Remington JS. False-positive results in immunoglobulin M (IgM) toxoplasma antibody tests and importance of confirmatory testing: the Platelia Toxo IgM test. J Clin Microbiol. 1997;35:174–8.
  PubMed PubMed Central CAS Google Scholar
• Chen X-G, Gong Y, Lun Z-R, Fung M-C. High-level expression and purification of immunogenic recombinant SAG1 (P30) of Toxoplasma gondii in Escherichia coli. Protein Expr Purif. 2001;23:33–7.
  Article PubMed CAS Google Scholar
• Kotresha D, Poonam D, Muhammad Hafiznur Y, Saadatnia G, Nurulhasanah O, Sabariah O, Tan S, Izzati Zahidah A, Rahmah N. Recombinant proteins from new constructs of SAG1 and GRA7 sequences and their usefulness to detect acute toxoplasmosis. Trop Biomed. 2012;29:129–37.
  PubMed CAS Google Scholar
• Prince JB, Auer KL, Huskinson J, Parmley SF, Araujo FG, Remington JS. Cloning, expression, and cDNA sequence of surface antigen P22 from Toxoplasma gondii. Mol Biochem Parasitol. 1990;43:97–106.
  Article PubMed CAS Google Scholar
• Pa Myjak. Efficient production of the Toxoplasma gondii GRA6, p35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis. Acta Parasitol. 2005;50:249–54.
  Google Scholar
• Khanaliha K, Motazedian M, Sarkari B, Bandehpour M, Sharifnia Z, Kazemi B. Expression and purification of P43 Toxoplasma gondii surface antigen. Iran J Parasitol. 2012;7:48–53.
  PubMed PubMed Central CAS Google Scholar
• Ferra B, Holec-Gąsior L, Kur J. A new Toxoplasma gondii chimeric antigen containing fragments of SAG2, GRA1, and ROP1 proteins—impact of immunodominant sequences size on its diagnostic usefulness. Parasitol Res. 2015;114:3291–9.
  Article PubMed PubMed Central Google Scholar
• Hiszczyńska-Sawicka E, Brillowska-Dąbrowska A, Dąbrowski S, Pietkiewicz H, Myjak P, Kur J. High yield expression and single-step purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli. Protein Expr Purif. 2003;27:150–7.
  Article PubMed Google Scholar
• Holec-Gąsior L, Kur J, Hiszczyńska-Sawicka E. GRA2 and ROP1 recombinant antigens as potential markers for detection of _Toxoplasma gondii_-specific immunoglobulin G in humans with acute toxoplasmosis. Clin Vaccine Immunol. 2009;16:510–4.
  Article PubMed PubMed Central CAS Google Scholar
• Ching XT, Lau YL, Fong MY, Nissapatorn V. Evaluation of _Toxoplasma gondii_-recombinant dense granular protein (GRA2) for serodiagnosis by western blot. Parasitol Res. 2013;112:1229–36.
  Article PubMed Google Scholar
• Mevelec M-N, Chardès T, Mercereau-Puijalon O, Bourguin I, Achbarou A, Dubremetz J-F, Bout D. Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies. Mol Biochem Parasitol. 1992;56:227–38.
  Article PubMed CAS Google Scholar
• Holec-Gąsior L, Kur J. Toxoplasma gondii: recombinant GRA5 antigen for detection of immunoglobulin G antibodies using enzyme-linked immunosorbent assay. Exp Parasitol. 2010;124:272–8.
  Article PubMed CAS Google Scholar
• Lecordier L, Moleon-Borodowsky I, Dubremetz J-F, Tourvieille B, Mercier C, Deslée D, Capron A, Cesbron-Delauw M-F. Characterization of a dense granule antigen of Toxoplasma gondii (GRA6) associated to the network of the parasitophorous vacuole. Mol Biochem Parasitol. 1995;70:85–94.
  Article PubMed CAS Google Scholar
• Redlich A, Müller WA. Serodiagnosis of acute toxoplasmosis using a recombinant form of the dense granule antigen GRA6 in an enzyme-linked immunosorbent assay. Parasitol Res. 1998;84:700–6.
  Article PubMed CAS Google Scholar
• Selseleh M, Keshavarz H, Mohebali M, Shojaee S, Selseleh M, Eshragian MR, Mansouri F, Modarressi MH. Production and evaluation of Toxoplasma gondii recombinant GRA7 for serodiagnosis of human infections. Korean J Parasitol. 2012;50:233–8.
  Article PubMed PubMed Central CAS Google Scholar
• Arab-Mazar Z, Fallahi S, Koochaki A, Haghighi A, Tabaei SJS. Immunodiagnosis and molecular validation of _Toxoplasma gondii_-recombinant dense granular (GRA) 7 protein for the detection of toxoplasmosis in patients with cancer. Microbiol Res. 2016;183:53–9.
  Article PubMed CAS Google Scholar
• Holec L, Gąsior A, Brillowska-Dąbrowska A, Kur J. Toxoplasma gondii: enzyme-linked immunosorbent assay using different fragments of recombinant microneme protein 1 (MIC1) for detection of immunoglobulin G antibodies. Exp Parasitol. 2008;119:1–6.
  Article PubMed CAS Google Scholar
• Kotresha D, Noordin R. Recombinant proteins in the diagnosis of toxoplasmosis. Apmis. 2010;118:529–42.
  PubMed CAS Google Scholar
• Holec L, Hiszczyńska-Sawicka E, Gąsior A, Brillowska-Dąbrowska A, Kur J. Use of MAG1 recombinant antigen for diagnosis of Toxoplasma gondii infection in humans. Clin Vaccine Immunol. 2007;14:220–5.
  Article PubMed PubMed Central CAS Google Scholar
• Van Gelder P, Bosman F, De Meuter F, Van Heuverswyn H, Hérion P. Serodiagnosis of toxoplasmosis by using a recombinant form of the 54-kilodalton rhoptry antigen expressed in Escherichia coli. J Clin Microbiol. 1993;31:9–15.
  PubMed PubMed Central Google Scholar
• Martin V, Arcavi M, Santillan G, Amendoeira MRR, Neves EDS, Griemberg G, Guarnera E, Garberi JC, Angel SO. Detection of human Toxoplasma-specific immunoglobulins A, M, and G with a recombinant Toxoplasma gondii rop2 protein. Clin Diagn Lab Immunol. 1998;5:627–31.
  PubMed PubMed Central CAS Google Scholar
• Holec-Gąsior L, Ferra B, Czechowska J, Serdiuk IE, Krzymiński K, Kur J. A novel chemiluminescent immunoassay for detection of Toxoplasma gondii IgG in human sera. Diagn Microbiol Infect Dis. 2016;85:422–5.
  Article PubMed CAS Google Scholar
• Pfrepper K-I, Enders G, Gohl M, Krczal D, Hlobil H, Wassenberg D, Soutschek E. Seroreactivity to and avidity for recombinant antigens in toxoplasmosis. Clin Diagn Lab Immunol. 2005;12:977–82.
  PubMed PubMed Central CAS Google Scholar
• Beghetto E, Buffolano W, Spadoni A, Del Pezzo M, Di Cristina M, Minenkova O, Petersen E, Felici F, Gargano N. Use of an immunoglobulin G avidity assay based on recombinant antigens for diagnosis of primary Toxoplasma gondii infection during pregnancy. J Clin Microbiol. 2003;41:5414–8.
  Article PubMed PubMed Central CAS Google Scholar
• Marcolino P, Silva D, Leser P, Camargo M, Mineo J. Molecular markers in acute and chronic phases of human toxoplasmosis: determination of immunoglobulin G avidity by Western blotting. Clin Diagn Lab Immunol. 2000;7:384–9.
  PubMed PubMed Central CAS Google Scholar
• Huang X, Xuan X, Hirata H, Yokoyama N, Xu L, Suzuki N, Igarashi I. Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats. J Clin Microbiol. 2004;42:351–3.
  Article PubMed PubMed Central CAS Google Scholar
• Terkawi MA, Kameyama K, Rasul NH, Xuan X, Nishikawa Y. Development of an immunochromatographic assay based on dense granule protein 7 for serological detection of Toxoplasma gondii infection. Clin Vaccine Immunol. 2013;20:596–601.
  Article PubMed PubMed Central CAS Google Scholar
• Meek B, Diepersloot RJ, van Gool T, Speijer D, Peek R. IgM recognition of recombinant Toxoplasma gondii antigens by sera of acutely or latently infected humans. Diagn Microbiol Infect Dis. 2003;45:45–52.
  Article PubMed CAS Google Scholar
• Holec-Gasior L, Drapala D, Lautenbach D, Kuri J. Toxoplasma gondii: usefulness of ROP1 recombinant antigen in an immunoglobulin G avidity assay for diagnosis of acute toxoplasmosis in humans. Pol J Microbiol. 2010;59:307–10.
  PubMed CAS Google Scholar
• Elyasi H, Babaie J, Fricker-Hidalgo H, Brenier-Pinchart M-P, Zare M, Sadeghiani G, Assmar M, Pelloux H, Golkar M. Use of dense granule antigen GRA6 in an immunoglobulin G avidity test to exclude acute Toxoplasma gondii infection during pregnancy. Clin Vaccine Immunol. 2010;17:1349–55.
  Article PubMed PubMed Central CAS Google Scholar
• Pietkiewicz H, Hiszczyńska-Sawicka E, Kur J, Petersen E, Nielsen H, Paul M, Stankiewicz M, Myjak P. Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7 and SAG1) in an immunoglobulin G avidity test for the serodiagnosis of toxoplasmosis. Parasitol Res. 2007;100:333–7.
  Article PubMed CAS Google Scholar
• Aubert D, Maine G, Villena I, Hunt J, Howard L, Sheu M, Brojanac S, Chovan L, Nowlan S, Pinon J. Recombinant antigens to detect _Toxoplasma gondii_-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay. J Clin Microbiol. 2000;38:1144–50.
  PubMed PubMed Central CAS Google Scholar
• Pietkiewicz H, Hiszczyńska-Sawicka E, Kur J, Petersen E, Nielsen H, Stankiewicz M, Andrzejewska I, Myjak P. Usefulness of _Toxoplasma gondii_-specific recombinant antigens in serodiagnosis of human toxoplasmosis. J Clin Microbiol. 2004;42:1779–81.
  Article PubMed PubMed Central CAS Google Scholar
• Buffolano W, Beghetto E, Del Pezzo M, Spadoni A, Di Cristina M, Petersen E, Gargano N. Use of recombinant antigens for early postnatal diagnosis of congenital toxoplasmosis. J Clin Microbiol. 2005;43:5916–24.
  Article PubMed PubMed Central CAS Google Scholar
• Li S, Galvan G, Araujo FG, Suzuki Y, Remington JS, Parmley S. Serodiagnosis of recently acquired Toxoplasma gondii infection using an enzyme-linked immunosorbent assay with a combination of recombinant antigens. Clin Diagn Lab Immunol. 2000;7:781–7.
  PubMed PubMed Central CAS Google Scholar
• Drapała D, Holec-Gąsior L, Kur J, Ferra B, Hiszczyńska-Sawicka E, Lautenbach D. A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis. Diagn Microbiol Infect Dis. 2014;79:342–6.
  Article PubMed CAS Google Scholar
• Maksimov P, Zerweck J, Maksimov A, Hotop A, Groß U, Pleyer U, Spekker K, Däubener W, Werdermann S, Niederstrasser O. Peptide microarray analysis of in silico-predicted epitopes for serological diagnosis of Toxoplasma gondii infection in humans. Clin Vaccine Immunol. 2012;19:865–74.
  Article PubMed PubMed Central CAS Google Scholar
• Beghetto E, Spadoni A, Bruno L, Buffolano W, Gargano N. Chimeric antigens of Toxoplasma gondii: toward standardization of toxoplasmosis serodiagnosis using recombinant products. J Clin Microbiol. 2006;44:2133–40.
  Article PubMed PubMed Central CAS Google Scholar
• Dai J, Jiang M, Wang Y, Qu L, Gong R, Si J. Evaluation of a recombinant multiepitope peptide for serodiagnosis of Toxoplasma gondii infection. Clin Vaccine Immunol. 2012;19:338–42.
  Article PubMed PubMed Central CAS Google Scholar
• Dai J-F, Jiang M, Qu L-L, Sun L, Wang Y-Y, Gong L-L, Gong R-J, Si J. Toxoplasma gondii: enzyme-linked immunosorbent assay based on a recombinant multi-epitope peptide for distinguishing recent from past infection in human sera. Exp Parasitol. 2013;133:95–100.
  Article PubMed CAS Google Scholar
• Holec-Gąsior L, Ferra B, Drapała D, Lautenbach D, Kur J. A new MIC1-MAG1 recombinant chimeric antigen can be used instead of the Toxoplasma gondii lysate antigen in serodiagnosis of human toxoplasmosis. Clin Vaccine Immunol. 2012;19:57–63.
  Article PubMed PubMed Central CAS Google Scholar
• Holec-Gąsior L, Ferra B, Drapała D. MIC1-MAG1-SAG1 chimeric protein, a most effective antigen for detection of human toxoplasmosis. Clin Vaccine Immunol. 2012;19:1977–9.
  Article PubMed PubMed Central CAS Google Scholar
• Lau YL, Thiruvengadam G, Lee WW, Fong MY. Immunogenic characterization of the chimeric surface antigen 1 and 2 (SAG1/2) of Toxoplasma gondii expressed in the yeast Pichia pastoris. Parasitol Res. 2011;109:871–8.
  Article PubMed Google Scholar
• Roggen EL. Recent developments with B-cell epitope identification for predictive studies. J Immunotoxicol. 2006;3:137–49.
  Article PubMed CAS Google Scholar
• Cardona N, de-la-Torre A, Siachoque H, Patarroyo MA, Gomez-Marin JE. Toxoplasma gondii: P30 peptides recognition pattern in human toxoplasmosis. Exp Parasitol. 2009;123:199–202.
  Article PubMed CAS Google Scholar
• Mévélec M, Mercereau-Puijalon O, Buzoni-Gatel D, Bourguin I, Chardès T, Dubremetz J, Bout D. Mapping of B epitopes in GRA4, a dense granule antigen of Toxoplasma gondii and protection studies using recombinant proteins administered by the oral route. Parasite Immunol. 1998;20:183–95.
  PubMed Google Scholar
• Reineke U. Antibody epitope mapping using de novo generated synthetic peptide libraries. Methods Mol Biol. 2009;524:203–11.
  Article PubMed CAS Google Scholar
• Switaj K, Master A, Skrzypczak M, Zaborowski P. Recent trends in molecular diagnostics for Toxoplasma gondii infections. Clin Microbiol Infect. 2005;11:170–6.
  Article PubMed CAS Google Scholar
• Gutierrez J, O’Donovan J, Williams E, Proctor A, Brady C, Marques P, Worrall S, Nally J, McElroy M, Bassett H. Detection and quantification of Toxoplasma gondii in ovine maternal and foetal tissues from experimentally infected pregnant ewes using real-time PCR. Vet Parasitol. 2010;172:8–15.
  Article PubMed CAS Google Scholar
• Fallahi S, Tabaei SJS, Pournia Y, Zebardast N, Kazemi B. Comparison of loop-mediated isothermal amplification (LAMP) and nested-PCR assay targeting the RE and B1 gene for detection of Toxoplasma gondii in blood samples of children with leukaemia. Diagn Microbiol Infect Dis. 2014;79:347–54.
  Article PubMed CAS Google Scholar
• Burg JL, Grover CM, Pouletty P, Boothroyd J. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol. 1989;27:1787–92.
  PubMed PubMed Central CAS Google Scholar
• Cazenave J, Cheyrou A, Blouin P, Johnson A, Begueret J. Use of polymerase chain reaction to detect Toxoplasma. J Clin Pathol. 1991;44:1037.
  Article PubMed PubMed Central CAS Google Scholar
• Homan W, Vercammen M, De Braekeleer J, Verschueren H. Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int J Parasitol. 2000;30:69–75.
  Article PubMed CAS Google Scholar
• Reischl U, Bretagne S, Krüger D, Ernault P, Costa J-M. Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect Dis. 2003;3:1.
  Article Google Scholar
• Fallahi S, Mazar ZA, Ghasemian M, Haghighi A. Challenging loop-mediated isothermal amplification (LAMP) technique for molecular detection of Toxoplasma gondii. Asian Pac J Trop Med. 2015;8:366–72.
  Article PubMed CAS PubMed Central Google Scholar
• Calderaro A, Piccolo G, Gorrini C, Peruzzi S, Zerbini L, Bommezzadri S, Dettori G, Chezzi C. Comparison between two real-time PCR assays and a nested-PCR for the detection of Toxoplasma gondii. Acta Biomedica-Ateneo Parmense. 2006;77:75.
  CAS Google Scholar
• Alonso R, Martinez E, Laynez P, Miguélez M, Pinero J, Valladares B. Detection by nested-PCR of Toxoplasma gondii in patients infected with human immunodeficiency virus. Medcina Clínica. 2002;118:294–6.
  Article Google Scholar
• Kourenti C, Karanis P. Evaluation and applicability of a purification method coupled with nested PCR for the detection of Toxoplasma oocysts in water. Lett Appl Microbiol. 2006;43:475–81.
  Article PubMed CAS Google Scholar
• Mousavi M, Saravani R, Modrek MJ, Shahrakipour M, Sekandarpour S. Detection of Toxoplasma gondii in diabetic patients using the nested PCR assay via RE and B1 genes. Jundishapur J Microbiol. 2016;9:e29493.
  PubMed PubMed Central Google Scholar
• Vitale M, Galluzzo P, Currò V, Gozdzik K, Schillaci D, Di Macro Lo Presti V. A high sensitive nested PCR for Toxoplasma gondii detection in animal and food samples. J Microb Biochem Technol. 2013;5:39–41.
  Article CAS Google Scholar
• Eskandarian AA. Development of an efficient multiplex semi-nested PCR for convenient use in urine samples for diagnosis of toxoplasmosis. MedicalExpress. 2014;1:39–42.
  Google Scholar
• Yamada H, Nishikawa A, Yamamoto T, Mizue Y, Yamada T, Morizane M, Tairaku S, Nishihira J. Prospective study of congenital toxoplasmosis screening with use of IgG avidity and multiplex nested PCR methods. J Clin Microbiol. 2011;49:2552–6.
  Article PubMed PubMed Central Google Scholar
• Martinez E, Carmelo E, Alonso R, Ortega A, Pinero J, Del Castillo A, Valladares B. Development of a rapid polymerase chain reaction-ELISA assay using polystyrene beads for the detection of Toxoplasma gondii DNA. Lett Appl Microbiol. 2003;36:30–4.
  Article PubMed CAS Google Scholar
• Nowakowska D, Colón I, Remington JS, Grigg M, Golab E, Wilczynski J, Sibley LD. Genotyping of Toxoplasma gondii by multiplex PCR and peptide-based serological testing of samples from infants in Poland diagnosed with congenital toxoplasmosis. J Clin Microbiol. 2006;44:1382–9.
  Article PubMed PubMed Central CAS Google Scholar
• Rahumatullah A, Khoo BY, Noordin R. Triplex PCR using new primers for the detection of Toxoplasma gondii. Exp Parasitol. 2012;131:231–8.
  Article PubMed CAS Google Scholar
• Mackay IM. Real-time PCR in the microbiology laboratory. Clin Microbiol Infect. 2004;10:190–212.
  Article PubMed CAS Google Scholar
• De Waal T. Advances in diagnosis of protozoan diseases. Vet Parasitol. 2012;189:65–74.
  Article PubMed Google Scholar
• Costa J-M, Pautas C, Ernault P, Foulet F, Cordonnier C, Bretagne S. Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes. J Clin Microbiol. 2000;38:2929–32.
  PubMed PubMed Central CAS Google Scholar
• Santos FF, Commodaro A, Nascimento HM, Furtado JM, Muccioli C, Rizzo LV, Belfort R Jr. Real-time PCR in the diagnosis of infectious posterior uveitis by toxoplasmosis. Invest Ophthalmol Vis Sci. 2014;55:5284.
  Article Google Scholar
• Costa JGL, Carneiro ACAV, Tavares AT, Andrade GMQ, Vasconcelos-Santos DV, Januário JN, Menezes-Souza D, Fujiwara RT, Vitor RWA. Real-time PCR as a prognostic tool for human congenital toxoplasmosis. J Clin Microbiol. 2013;51:2766–8.
  Article PubMed PubMed Central CAS Google Scholar
• Juránková J, Opsteegh M, Neumayerová H, Kovařčík K, Frencová A, Baláž V, Volf J, Koudela B. Quantification of Toxoplasma gondii in tissue samples of experimentally infected goats by magnetic capture and real-time PCR. Vet Parasitol. 2013;193:95–9.
  Article PubMed CAS Google Scholar
• Wells B, Shaw H, Innocent G, Guido S, Hotchkiss E, Parigi M, Opsteegh M, Green J, Gillespie S, Innes EA. Molecular detection of Toxoplasma gondii in water samples from Scotland and a comparison between the 529 bp real-time PCR and ITS1 nested PCR. Water Res. 2015;87:175–81.
  Article PubMed CAS Google Scholar
• Juránková J, Basso W, Neumayerová H, Frencová A, Baláž V, Deplazes P, Koudela B. Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection. Food Microbiol. 2015;52:150–3.
  Article PubMed CAS Google Scholar
• Bastien P, Procop GW, Reischl U. Quantitative real-time PCR is not more sensitive than “conventional” PCR. J Clin Microbiol. 2008;46:1897–900.
  Article PubMed PubMed Central CAS Google Scholar
• Kompalic-Cristo A, Frotta C, Suárez-Mutis M, Fernandes O, Britto C. Evaluation of a real-time PCR assay based on the repetitive B1 gene for the detection of Toxoplasma gondii in human peripheral blood. Parasitol Res. 2007;101:619–25.
  Article PubMed Google Scholar
• Lin M-H, Chen T-C, Kuo T-T, Tseng C-C, Tseng C-P. Real-time PCR for quantitative detection of Toxoplasma gondii. J Clin Microbiol. 2000;38:4121–5.
  PubMed PubMed Central CAS Google Scholar
• Delhaes L, Yera H, Ache S, Tsatsaris V, Houfflin-Debarge V. Contribution of molecular diagnosis to congenital toxoplasmosis. Diagn Microbiol Infect Dis. 2013;76:244–7.
  Article PubMed CAS Google Scholar
• Juránková J, Basso W, Neumayerová H, Baláž V, Jánová E, Sidler X, Deplazes P, Koudela B. Brain is the predilection site of Toxoplasma gondii in experimentally inoculated pigs as revealed by magnetic capture and real-time PCR. Food Microbiol. 2014;38:167–70.
  Article PubMed CAS Google Scholar
• Abbasi I, King CH, Muchiri EM, Hamburger J. Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: identification of infected snails from early prepatency. Am J Trop Med Hyg. 2010;83:427–32.
  Article PubMed PubMed Central CAS Google Scholar
• Alhassan A, Thekisoe OM, Yokoyama N, Inoue N, Motloang MY, Mbati PA, Yin H, Katayama Y, Anzai T, Sugimoto C. Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis. Vet Parasitol. 2007;143:155–60.
  Article PubMed Google Scholar
• Gallas-Lindemann C, Sotiriadou I, Mahmoodi MR, Karanis P. Detection of Toxoplasma gondii oocysts in different water resources by loop mediated isothermal amplification (LAMP). Acta Trop. 2013;125:231–6.
  Article PubMed CAS Google Scholar
• Abdul-Ghani R, Al-Mekhlafi AM, Karanis P. Loop-mediated isothermal amplification (LAMP) for malarial parasites of humans: would it come to clinical reality as a point-of-care test? Acta Trop. 2012;122:233–40.
  Article PubMed CAS Google Scholar
• Mori Y, Notomi T. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother. 2009;15:62–9.
  Article PubMed CAS PubMed Central Google Scholar
• Poon LL, Wong BW, Ma EH, Chan KH, Chow LM, Abeyewickreme W, Tangpukdee N, Yuen KY, Guan Y, Looareesuwan S. Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem. 2006;52:303–6.
  Article PubMed CAS Google Scholar
• Zhang H, Thekisoe OM, Aboge GO, Kyan H, Yamagishi J, Inoue N, Nishikawa Y, Zakimi S, Xuan X. Toxoplasma gondii: sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method. Exp Parasitol. 2009;122:47–50.
  Article PubMed CAS Google Scholar
• Zhang X, Lowe SB, Gooding JJ. Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP). Biosens Bioelectron. 2014;61:491–9.
  Article PubMed CAS Google Scholar
• Wang Y, Wang G, Zhang D, Yin H, Wang M. Detection of acute toxoplasmosis in pigs using loop-mediated isothermal amplification and quantitative PCR. Korean J Parasitol. 2013;51:573–7.
  Article PubMed PubMed Central CAS Google Scholar
• Lin Z, Zhang Y, Zhang H, Zhou Y, Cao J, Zhou J. Comparison of loop-mediated isothermal amplification (LAMP) and real-time PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis. Vet Parasitol. 2012;185:296–300.
  Article PubMed CAS Google Scholar
• Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28:e63.
  Article PubMed PubMed Central CAS Google Scholar
• Sotiriadou I, Karanis P. Evaluation of loop-mediated isothermal amplification for detection of Toxoplasma gondii in water samples and comparative findings by polymerase chain reaction and immunofluorescence test (IFT). Diagn Microbiol Infect Dis. 2008;62:357–65.
  Article PubMed CAS Google Scholar
• Qu D, Zhou H, Han J, Tao S, Zheng B, Chi N, Su C, Du A. Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a diagnostic tool of Toxoplasma gondii in pork. Vet Parasitol. 2013;192:98–103.
  Article PubMed CAS Google Scholar
• Lau YL, Meganathan P, Sonaimuthu P, Thiruvengadam G, Nissapatorn V, Chen Y. Specific, sensitive, and rapid diagnosis of active toxoplasmosis by a loop-mediated isothermal amplification method using blood samples from patients. J Clin Microbiol. 2010;48:3698–702.
  Article PubMed PubMed Central CAS Google Scholar
• Hu X, Pan C-W, Li Y-F, Wang H, Tan F. Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP). Folia Parasitol. 2012;59:21.
  Article CAS Google Scholar
• Kong Q-M, Lu S-H, Tong Q-B, Lou D, Chen R, Zheng B, Kumagai T, Wen L-Y, Ohta N, Zhou X-N. Loop-mediated isothermal amplification (LAMP): early detection of Toxoplasma gondii infection in mice. Parasites Vectors. 2012;5:1.
  Article CAS Google Scholar
• Smith AB, Smirniotopoulos JG, Rushing EJ. Central nervous system infections associated with human immunodeficiency virus infection: radiologic–pathologic correlation 1. Radiographics. 2008;28:2033–58.
  Article PubMed Google Scholar
• Scheld MW, Whitley RJ, Marra CM. Infections of the central nervous system. Philadelphia: Lippincott Williams & Wilkins; 2014.
  Google Scholar
• Masamed R, Meleis A, Lee E, Hathout G. Cerebral toxoplasmosis: case review and description of a new imaging sign. Clin Radiol. 2009;64:560–3.
  Article PubMed CAS Google Scholar
• Brightbill T, Post MJD, Hensley GT, Ruiz A. MR of Toxoplasma encephalitis: signal characteristics on T2-weighted images and pathologic correlation. J Comput Assist Tomogr. 1996;20:417–22.
  Article PubMed CAS Google Scholar
• Khan AN, Smirniotopoulos JG. Imaging in CNS toxoplasmosis. 2015. https://emedicine.medscape.com/article/344706-overview. Accessed 15 Nov 2017.
• Hoffman JM, Waskin HA, Schifter T, Hanson MW, Gray L, Rosenfeld S, Coleman RE. FDG-PET in differentiating lymphoma from nonmalignant central nervous system lesions in patients with AIDS. J Nucl Med Off Publ Soc Nucl Med. 1993;34:567–75.
  CAS Google Scholar
• Villringer K, Jäger H, Dichgans M, Ziegler S, Poppinger J, Herz M, Kruschke C, Minoshima S, Pfister HW, Schwaiger M. Differential diagnosis of CNS lesions in AIDS patients by FDG-PET. J Comput Assist Tomogr. 1995;19:532–6.
  Article PubMed CAS Google Scholar
• Kim HW, Won KS, Choi BW, Zeon SK. Cerebral toxoplasmosis in a patient with AIDS on F-18 FDG PET/CT. Nucl Med Mol Imaging. 2010;44:75–7.
  Article PubMed PubMed Central Google Scholar
• D’Ercole C, Girard N, Boubli L, Potier A, Chagnon C, Raybaud C, Blanc B. Prenatal diagnosis of fetal cerebral abnormalities by ultrasonography and magnetic resonance imaging. Eur J Obstet Gynecol Reprod Biol. 1993;50:177–84.
  Article PubMed Google Scholar
• Lago E, Baldisserotto M, Hoefel Filho J, Santiago D, Jungblut R. Agreement between ultrasonography and computed tomography in detecting intracranial calcifications in congenital toxoplasmosis. Clin Radiol. 2007;62:1004–11.
  Article PubMed CAS Google Scholar
• Abboud P, Harika G, Saniez D, Gabriel R, Bednarczyk L, Chemla C, Quereux C. Ultrasonic signs of fetal toxoplasmosis. Review of the literature. J Gynecol Obstet Biol Reprod. 1994;24:733–8.
  Google Scholar
• Lavinsky D, Romano A, Muccioli C, Belfort R Jr. Imaging in ocular toxoplasmosis. Int Ophthalmol Clin. 2012;52:131–43.
  Article PubMed Google Scholar