Cell Adhesion Molecule Expression in the Cervix and... : Obstetrics & Gynecology (original) (raw)

ORIGINAL RESEARCH

Cell Adhesion Molecule Expression in the Cervix and Myometrium During Pregnancy and Parturition

LEDINGHAM, M. A. MRCOG; THOMSON, A. J. MD, MRCOG; JORDAN, F. MSc; YOUNG, A.; CRAWFORD, M.; NORMAN, J. E. MD, MRCOG

Department of Obstetrics and Gynaecology, University of Glasgow, Glasgow, Scotland, United Kingdom.

Address reprint requests to: Marie-Anne Ledingham, MRCOG, Specialist Registrar, Department of Obstetrics and Gynaecology, University of Glasgow, 10 Alexandra Parade, Glasgow, Scotland G31 2ER, United Kingdom. E-mail: [email protected]

This work was funded by Scottish Health Endowments Research Trust (grants 1442 and RG64/99) and by TENOVUS Scotland (grant S99/2).

Received June 7, 2000. Received in revised form September 27, 2000. Accepted October 12, 2000.

Objective

To determine the expression and localization of cell adhesion molecules intercellular adhesion molecule-1 (ICAM-1), E-selectin, platelet–endothelial cell adhesion molecule (PECAM), and vascular cell adhesion molecule (VCAM) in the cervix and myometrium during pregnancy and labor.

Methods

Biopsies of myometrium and cervix were obtained from non-pregnant women and from pregnant women before and after onset of spontaneous labor at term. Cell adhesion molecule mRNA expression was quantified using Northern blotting and cell adhesion molecule protein was localized using immunohistochemistry.

Results

ICAM-1 mRNA was upregulated in the cervix (10-fold increase, P < .01) and myometrium (10.5-fold increase, P < .01) during labor. ICAM-1 was localized in the vascular endothelium and in leukocytes in the cervix and myometrium from all three groups of women. VCAM mRNA was upregulated in the cervix (2.5-fold increase, P < .01) during pregnancy and there was no further change during labor. VCAM localized weakly to the vascular endothelium in cervical and myometrial biopsies from pregnant and non-pregnant women. PECAM mRNA was significantly upregulated in myometrium during pregnancy (ninefold increase, P < .01) and did not change with the onset of labor. PECAM localized to the vascular endothelium in all cervical and myometrial biopsies and was identified on leukocytes. There were no significant changes in E-selectin mRNA expression in either tissue with pregnancy or parturition.

Conclusion

Cell adhesion molecule expression changes in human cervix and myometrium during pregnancy and parturition. At least part of these changes are attributable to expression by leukocytes infiltrating these tissues.