Molecular characterization of Coxiella burnetii isolates | Epidemiology & Infection | Cambridge Core (original) (raw)
Hostname: page-component-745bb68f8f-5r2nc Total loading time: 0 Render date: 2025-01-23T19:05:35.693Z Has data issue: false hasContentIssue false
Published online by Cambridge University Press: 01 March 1998
C. JÄGER
Affiliation:
Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University, Giessen, Germany
H. WILLEMS
Affiliation:
Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University, Giessen, Germany
D. THIELE
Affiliation:
Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University, Giessen, Germany
G. BALJER
Affiliation:
Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University, Giessen, Germany
Abstract
Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Restriction fragment length polymorphism (RFLP) was used for the differentiation of 80 Coxiella burnetii isolates derived from animals and humans in Europe, USA, Africa and Asia. After NotI restriction of total C. burnetii DNA and pulsed field gel electrophoresis (PFGE) 20 different restriction patterns were distinguished. The index of discrimination for this typing system was 0·86. Comparison and phylogenetic analysis of the different RFLP patterns revealed evolutionary relationships among groups that corresponded to the geographical origin of the isolates. This finding was confirmed by genetic mapping. No correlation between restriction group and virulence of isolates was detected.
Type
Research Article
Copyright
1998 Cambridge University Press