Protein Adsorption of Ultrafine Metal Oxide and Its Influence on Cytotoxicity toward Cultured Cells (original) (raw)

ArticleFebruary 16, 2009

Protein Adsorption of Ultrafine Metal Oxide and Its Influence on Cytotoxicity toward Cultured Cells

Click to copy article linkArticle link copied!

Chemical Research in Toxicology

Cite this: Chem. Res. Toxicol. 2009, 22, 3

Click to copy citationCitation copied!

Published February 16, 2009

research-article

Copyright © 2009 American Chemical Society

Abstract

Click to copy section linkSection link copied!

Abstract Image

Many investigations about the cellular response by metal oxide nanoparticles in vitro have been reported. However, the influence of the adsorption ability of metal oxide nanoparticles toward cells is unknown. The aim of this study is to understand the influence of adsorption by metal oxide nanoparticles on the cell viability in vitro. The adsorption abilities of six kinds of metal oxide nanoparticles, namely, NiO, ZnO, TiO2, CeO2, SiO2, and Fe2O3, to Dulbecco’s modified Eagle’s medium supplemented with a 10% fetal bovine serum (DMEM-FBS) component such as serum proteins and Ca2+ were estimated. All of the metal oxide nanoparticles adsorbed proteins and Ca2+ in the DMEM-FBS; in particular, TiO2, CeO2, and ZnO showed strong adsorption abilities. Furthermore, the influence of the depletion of medium components by adsorption to metal oxide nanoparticles on cell viability and proliferation was examined. The particles were removed from the dispersion by centrifugation, and the supernatant was applied to the cells. Both the cell viability and the proliferation of human keratinocyte HaCaT cells and human lung carcinoma A549 cells were affected by the supernatant. In particular, cell proliferation was strongly inhibited by the supernatant of TiO2 and CeO2 dispersions. The supernatant showed depletion of serum proteins and Ca2+ by adsorption to metal oxide nanoparticles. When the adsorption effect was blocked by the pretreatment of particles with FBS, the inhibitory effect was lost. However, in NiO and ZnO, which showed ion release, a decrease of inhibitory effect by pretreatment was not shown. Furthermore, the association of the primary particle size and adsorption ability was examined in TiO2. The adsorption ability of TiO2 depended on the primary particle size. The TiO2 nanoparticles were size dependently absorbed with proteins and Ca2+, thereby inducing cytotoxicity. In conclusion, the adsorption ability of metal oxide nanoparticles is an important factor for the estimation of cytotoxicity in vitro for low-toxicity materials.

ACS Publications

Copyright © 2009 American Chemical Society

Cited By

Click to copy section linkSection link copied!

This article is cited by 246 publications.

  1. Taisiya O. Kozlova, Ekaterina D. Sheichenko, Daniil A. Kozlov, Irina V. Savintseva, Madina M. Sozarukova, Irina V. Kolesnik, Anton L. Popov, Darya N. Vasilyeva, Olga S. Ivanova, Alexander E. Baranchikov. Potential of (NH4)2Ce(PO4)2·H2O Phase for the Next Generation of Sunscreens: UV-Shielding Properties, Cytotoxicity and Biochemical Behavior. ACS Applied Bio Materials 2026, 9 (6) , 2914-2927. https://doi.org/10.1021/acsabm.5c02105
  2. Toni Vitasovic, Giada Caniglia, Neda Eghtesadi, Marcel Ceccato, Espen Drath Bo̷jesen, Ulrich Gosewinkel, Gregor Neusser, Ulrich Rupp, Paul Walther, Christine Kranz, Elena E. Ferapontova. Antibacterial Action of Zn2+ Ions Driven by the In Vivo Formed ZnO Nanoparticles. ACS Applied Materials & Interfaces 2024, 16 (24) , 30847-30859. https://doi.org/10.1021/acsami.4c04682
  3. Elijah. J. Petersen, Ana C. Barrios, Theodore B. Henry, Monique E. Johnson, Albert A. Koelmans, Antonio R. Montoro Bustos, Joanna Matheson, Matthias Roesslein, Jian Zhao, Baoshan Xing. Potential Artifacts and Control Experiments in Toxicity Tests of Nanoplastic and Microplastic Particles. Environmental Science & Technology 2022, 56 (22) , 15192-15206. https://doi.org/10.1021/acs.est.2c04929
  4. Romi Singh Maharjan, Ajay Vikram Singh, Javaria Hanif, Daniel Rosenkranz, Rashad Haidar, Amruta Shelar, Shubham Pratap Singh, Aditya Dey, Rajendra Patil, Paolo Zamboni, Peter Laux, Andreas Luch. Investigation of the Associations between a Nanomaterial’s Microrheology and Toxicology. ACS Omega 2022, 7 (16) , 13985-13997. https://doi.org/10.1021/acsomega.2c00472
  5. Renaud Passieux, Guillaume Sudre, Alexandra Montembault, Martine Renard, Agnès Hagege, Pierre Alcouffe, Ali Haddane, Marie Vandesteene, Nadège Boucard, Laurence Bordenave, Laurent David. Cytocompatibility / Antibacterial Activity Trade-off for Knittable Wet-Spun Chitosan Monofilaments Functionalized by the In Situ Incorporation of Cu2+ and Zn2+. ACS Biomaterials Science & Engineering 2022, 8 (4) , 1735-1748. https://doi.org/10.1021/acsbiomaterials.2c00079
  6. Aurica Precupas, Daniela Gheorghe, Alina Botea-Petcu, Anca Ruxandra Leonties, Romica Sandu, Vlad Tudor Popa, Espen Mariussen, El Yamani Naouale, Elise Rundén-Pran, Veronica Dumit, Ying Xue, Mihaela Roxana Cimpan, Maria Dusinska, Andrea Haase, Speranta Tanasescu. Thermodynamic Parameters at Bio–Nano Interface and Nanomaterial Toxicity: A Case Study on BSA Interaction with ZnO, SiO2, and TiO2. Chemical Research in Toxicology 2020, 33 (8) , 2054-2071. https://doi.org/10.1021/acs.chemrestox.9b00468
  7. Veronica Montesinos-Cruz, Jordan Rose, Aglaia Pappa, Mihalis I. Panayiotidis, Andrea De Vizcaya-Ruiz, Rodrigo Franco. Survival Mechanisms and Xenobiotic Susceptibility of Keratinocytes Exposed to Metal-Derived Nanoparticles. Chemical Research in Toxicology 2020, 33 (2) , 536-552. https://doi.org/10.1021/acs.chemrestox.9b00398
  8. Blaise L. Tardy, Joseph J. Richardson, Vichida Nithipipat, Kristian Kempe, Junling Guo, Kwun Lun Cho, Md. Arifur Rahim, Hirotaka Ejima, Frank Caruso. Protein Adsorption and Coordination-Based End-Tethering of Functional Polymers on Metal–Phenolic Network Films. Biomacromolecules 2019, 20 (3) , 1421-1428. https://doi.org/10.1021/acs.biomac.9b00006
  9. Nandita Menon and David T. Leong . Cytotoxic Effects of Phosphonate-Functionalized Mesoporous Silica Nanoparticles. ACS Applied Materials & Interfaces 2016, 8 (3) , 2416-2422. https://doi.org/10.1021/acsami.5b11741
  10. Doris Antoinette Mbeh, Laura Karina Mireles, Dimitri Stanicki, Lyes Tabet, Karim Maghni, Sophie Laurent, Edward Sacher, and L’Hocine Yahia . Human Alveolar Epithelial Cell Responses to Core–Shell Superparamagnetic Iron Oxide Nanoparticles (SPIONs). Langmuir 2015, 31 (13) , 3829-3839. https://doi.org/10.1021/la5040646
  11. Anoop K. Pal, Iraj Aalaei, Suresh Gadde, Peter Gaines, Daniel Schmidt, Philip Demokritou, and Dhimiter Bello . High Resolution Characterization of Engineered Nanomaterial Dispersions in Complex Media Using Tunable Resistive Pulse Sensing Technology. ACS Nano 2014, 8 (9) , 9003-9015. https://doi.org/10.1021/nn502219q
  12. Elijah J. Petersen, Theodore B. Henry, Jian Zhao, Robert I. MacCuspie, Teresa L. Kirschling, Marina A. Dobrovolskaia, Vincent Hackley, Baoshan Xing, and Jason C. White . Identification and Avoidance of Potential Artifacts and Misinterpretations in Nanomaterial Ecotoxicity Measurements. Environmental Science & Technology 2014, 48 (8) , 4226-4246. https://doi.org/10.1021/es4052999
  13. Jens Rauch, Walter Kolch, Sophie Laurent, and Morteza Mahmoudi . Big Signals from Small Particles: Regulation of Cell Signaling Pathways by Nanoparticles. Chemical Reviews 2013, 113 (5) , 3391-3406. https://doi.org/10.1021/cr3002627
  14. Raju Y. Prasad, Kathleen Wallace, Kaitlin M. Daniel, Alan H. Tennant, Robert M. Zucker, Jenna Strickland, Kevin Dreher, Andrew D. Kligerman, Carl F. Blackman, and David M. DeMarini . Effect of Treatment Media on the Agglomeration of Titanium Dioxide Nanoparticles: Impact on Genotoxicity, Cellular Interaction, and Cell Cycle. ACS Nano 2013, 7 (3) , 1929-1942. https://doi.org/10.1021/nn302280n
  15. Kirsten Gerloff, Ivana Fenoglio, Emanuele Carella, Julia Kolling, Catrin Albrecht, Agnes W. Boots, Irmgard Förster, and Roel P. F. Schins . Distinctive Toxicity of TiO2 Rutile/Anatase Mixed Phase Nanoparticles on Caco-2 Cells. Chemical Research in Toxicology 2012, 25 (3) , 646-655. https://doi.org/10.1021/tx200334k
  16. Masanori Horie, Haruhisa Kato, Katsuhide Fujita, Shigehisa Endoh, and Hitoshi Iwahashi . In Vitro Evaluation of Cellular Response Induced by Manufactured Nanoparticles. Chemical Research in Toxicology 2012, 25 (3) , 605-619. https://doi.org/10.1021/tx200470e
  17. Katherine M. Buettner and Ann M. Valentine . Bioinorganic Chemistry of Titanium. Chemical Reviews 2012, 112 (3) , 1863-1881. https://doi.org/10.1021/cr1002886
  18. Seung Soo Lee, Huiguang Zhu, Elizabeth Q. Contreras, Arjun Prakash, Hema L. Puppala, and Vicki L. Colvin . High Temperature Decomposition of Cerium Precursors To Form Ceria Nanocrystal Libraries for Biological Applications. Chemistry of Materials 2012, 24 (3) , 424-432. https://doi.org/10.1021/cm200863q
  19. Ivana Fenoglio, Elisabetta Aldieri, Elena Gazzano, Federico Cesano, Massimiliano Colonna, Domenica Scarano, Gianna Mazzucco, Angelo Attanasio, Yousof Yakoub, Dominique Lison, and Bice Fubini . Thickness of Multiwalled Carbon Nanotubes Affects Their Lung Toxicity. Chemical Research in Toxicology 2012, 25 (1) , 74-82. https://doi.org/10.1021/tx200255h
  20. Morteza Mahmoudi, Iseult Lynch, Mohammad Reza Ejtehadi, Marco P. Monopoli, Francesca Baldelli Bombelli, and Sophie Laurent . Protein−Nanoparticle Interactions: Opportunities and Challenges. Chemical Reviews 2011, 111 (9) , 5610-5637. https://doi.org/10.1021/cr100440g
  21. Geert Cornelis, Brooke Ryan, Mike J. McLaughlin, Jason K. Kirby, Douglas Beak, and David Chittleborough . Solubility and Batch Retention of CeO2 Nanoparticles in Soils. Environmental Science & Technology 2011, 45 (7) , 2777-2782. https://doi.org/10.1021/es103769k
  22. Karin H. Müller, Jaideep Kulkarni, Michael Motskin, Angela Goode, Peter Winship, Jeremy N. Skepper, Mary P. Ryan, and Alexandra E. Porter . pH-Dependent Toxicity of High Aspect Ratio ZnO Nanowires in Macrophages Due to Intracellular Dissolution. ACS Nano 2010, 4 (11) , 6767-6779. https://doi.org/10.1021/nn101192z
  23. Zhaoxia Ji, Xue Jin, Saji George, Tian Xia, Huan Meng, Xiang Wang, Elizabeth Suarez, Haiyuan Zhang, Eric M.V. Hoek, Hilary Godwin, André E. Nel, and Jeffrey I. Zink . Dispersion and Stability Optimization of TiO2 Nanoparticles in Cell Culture Media. Environmental Science & Technology 2010, 44 (19) , 7309-7314. https://doi.org/10.1021/es100417s
  24. Melissa A. Maurer-Jones, Yu-Shen Lin and Christy L. Haynes. Functional Assessment of Metal Oxide Nanoparticle Toxicity in Immune Cells. ACS Nano 2010, 4 (6) , 3363-3373. https://doi.org/10.1021/nn9018834
  25. Masanori Horie, Keiko Nishio, Katsuhide Fujita, Haruhisa Kato, Ayako Nakamura, Shinichi Kinugasa, Shigehisa Endoh, Arisa Miyauchi, Kazuhiro Yamamoto, Hideki Murayama, Etsuo Niki, Hitoshi Iwahashi, Yasukazu Yoshida and Junko Nakanishi. Ultrafine NiO Particles Induce Cytotoxicity in Vitro by Cellular Uptake and Subsequent Ni(II) Release. Chemical Research in Toxicology 2009, 22 (8) , 1415-1426. https://doi.org/10.1021/tx900171n
  26. Vilas P. Chaudhari, Debesh R. Roy, Sutapa Mondal Roy. NiS 2 nanoparticles: structural, spectroscopic and significant antibacterial properties against series of pathogenic strains. Preparative Biochemistry & Biotechnology 2026, 56 (5) , 692-700. https://doi.org/10.1080/10826068.2025.2562320
  27. Mohd Talha. Corrosion resistance and biofunctionalization of implant surfaces via metal oxide nanostructures: An emerging strategies review. FlatChem 2026, 57 , 101022. https://doi.org/10.1016/j.flatc.2026.101022
  28. Khaled Y. Abdel-Halim, Soad M. Mohy El-Din, Nadia H. Noaman, Manal M. El-Abasy. Oxidative stress and osmolyte induction caused by a Thiobencarb nano-emulsion in the freshwater alga Chlorella vulgaris. Ecotoxicology 2026, 35 (4)https://doi.org/10.1007/s10646-026-03041-8
  29. Vivian Inês dos Santos, Shahin Homaeigohar, Aldo Roberto Boccaccini. Metal and Metal Oxide Nanoparticle-incorporated Polymer Nanofibers for Wound Healing. 2025, 100-178. https://doi.org/10.1039/9781837677627-00100
  30. Xiaoqing Lu, Zhuying Yan, Fang Hao, Zhenyu Wang, Xianzheng Yuan, Xiaofan Yang, Tao Chen, Xiuping Yan. Importance of control experiments in toxicity test of metal–organic frameworks: Recent advances and challenges. Eco-Environment & Health 2025, 4 (4) , 100191. https://doi.org/10.1016/j.eehl.2025.100191
  31. Rekha Pilliadugula, Jebiti Haribabu, Mohamed Kasim Mohamed Subarkhan, Ramalinga Viswanathan Mangalaraja, Cesar Echeverria, Subhankar Mishra, N. Gopalakrishnan. Pernicious effect of hydrothermally synthesized pure and doped (Sn, Cr) β-Ga2O3 on various cancer cells through apoptosis. Inorganic Chemistry Communications 2025, 179 , 114770. https://doi.org/10.1016/j.inoche.2025.114770
  32. T. O. Kozlova, D. N. Vasilyeva, I. V. Savintseva, A. L. Popov, N. P. Simonenko, D. A. Kozlov. Hydrothermal Synthesis of K2Ce(PO4)2·хH2O and Analysis of Its Photoprotective Properties. Russian Journal of Inorganic Chemistry 2025, 70 (8) , 1140-1149. https://doi.org/10.1134/S0036023625602223
  33. Ardhendu Kumar Mandal. Nickel nanomaterials as delivery system in combating diseases. Journal of Drug Delivery and Therapeutics 2025, 15 (5) , 166-176. https://doi.org/10.22270/jddt.v15i5.7124
  34. Mashilo Matotoka, Peter Masoko. In Vitro Cytotoxicity Determination: Avoiding Pitfalls. 2025https://doi.org/10.5772/intechopen.1008312
  35. Rin Ichinohe, Ryosuke Segawa, Takahisa Nakajo, Masahiro Hiratsuka, Takeharu Yoshii, Kritin Pirabul, Zhen‐Ze Pan, Hirotomo Nishihara, Noriyasu Hirasawa. Carbon‐Based Particles Inhibit Antigen Penetration Into the Skin by Adsorbing the Antigen. Experimental Dermatology 2025, 34 (2)https://doi.org/10.1111/exd.70061
  36. Elena G. Varlamova, Sergey V. Gudkov, Egor A. Turovsky. Differential effect of cerium nanoparticles on the viability, redox-status and Ca2+-signaling system of cancer cells of various origins. Archives of Biochemistry and Biophysics 2025, 764 , 110261. https://doi.org/10.1016/j.abb.2024.110261
  37. Gloria Salinas-Lucero, Karla Juarez-Moreno, Rafael Vazquez-Duhalt. Cloud point extraction and characterization of zinc oxide nanoparticles isolated from infant milk formulas. Journal of Food Measurement and Characterization 2024, 18 (11) , 9330-9340. https://doi.org/10.1007/s11694-024-02881-4
  38. N. Rodríguez-Barajas, M.D. Ponce-Regalado, M.S. Segura-Almendárez, C.M. Rodríguez-Razon, Suresh Ghotekar, Mamoun Fellah, A. Pérez-Larios. Plant-mediated synthesis and interaction of ZnO against breast and prostate cancer: Review. Results in Chemistry 2024, 9 , 101654. https://doi.org/10.1016/j.rechem.2024.101654
  39. Manar A. Hammoud, Karkaz M. Thalij, Ahmed A. Alnazal. Effective Zn-NPs and Conjugates of Nisin and Natamycin Against Food Poisoning Microbes. IOP Conference Series: Earth and Environmental Science 2024, 1371 (6) , 062040. https://doi.org/10.1088/1755-1315/1371/6/062040
  40. Sneha Asha, Manimehala Udhayasuriyan, Minsa Mini, S. V. Divyalekshmi, Pooja P. Rajan, Aparna Mohan, T. S. Xavier, Praveen Kumar. Phytomediated Synthesis of Zinc Oxide (ZnO) Nanoparticles via Vitex negundo Leaf Extract: Characterization, Antibacterial and Antibiofilm Activity. Nano 2024, 19 (04)https://doi.org/10.1142/S1793292024500152
  41. Ankita Nandi, Ritam Mehera, Moumita Mandal, Paramesh Chandra, Swapan K. Mandal, Naznin Ara Begum, Chandan K. Jana, Nilanjana Das. Effects of biosynthesized ZnO nanoparticles on oxidative stress parameters in Saccharomyces cerevisiae. Journal of Physics and Chemistry of Solids 2024, 185 , 111748. https://doi.org/10.1016/j.jpcs.2023.111748
  42. Nataliya Kavok, Vladimir Klochkov, Katheryna Averchenko, Ganna Grygorova, Olga Sedyh, Svetlana Yefimova. Stability optimization of orthovanadate nanoparticles in biocompatible media. Journal of Dispersion Science and Technology 2023, 45 (1) , 161-170. https://doi.org/10.1080/01932691.2022.2159832
  43. Tz-ju Hong, Chandrasekar Sivakumar, Chih-Wei Luo, Mon-Shu Ho. Investigation of TiO2 nanoparticle interactions in the fibroblast NIH-3T3 cells via liquid-mode atomic force microscope. Archives of Toxicology 2023, 97 (11) , 2893-2901. https://doi.org/10.1007/s00204-023-03585-2
  44. Ayushi Priyam, Prerna Seth, Jibanananda Mishra, Palash Kumar Manna, Pushplata Prasad Singh. Occupational safety assessment of biogenic urea nanofertilisers using in vitro pulmonary, and in vivo ocular models. Heliyon 2023, 9 (11) , e21623. https://doi.org/10.1016/j.heliyon.2023.e21623
  45. Saad Alghamdi, Krisha Khandelwal, Soumya Pandit, Arpita Roy, Subhasree Ray, Ahad Amer Alsaiari, Abdulelah Aljuaid, Mazen Almehmadi, Mamdouh Allahyani, Rohit Sharma, Jigisha Anand, Ahmad Adnan Alshareef. Application of nanomaterials as potential quorum quenchers for disease: Recent advances and challenges. Progress in Biophysics and Molecular Biology 2023, 184 , 13-31. https://doi.org/10.1016/j.pbiomolbio.2023.08.005
  46. Muthuchamy Maruthupandy, Muruganantham Rethinasabapathy, Soyeon Jeon, Jiyoung Jeong, Eunsu Kim, Sinuk Lee, Songyeon Kim, Gyuri Kim, Yeonjeong Ha, Eunsol Bae, Yun Suk Huh, Wan-Seob Cho. Role of reactive oxygen species in the toxicity of two-dimensional nanomaterials: A study on layered Ti3C2 MXenes. Nano Today 2023, 51 , 101925. https://doi.org/10.1016/j.nantod.2023.101925
  47. Peng Zhu, Han Yin, Jiajiao Wei, Jianmeng Wu, Dehai Ping, Xingqun Zhang. A bilayer biocompatible polycaprolactone/zinc oxide/Capparis spinosa L. ethyl acetate extract/polylactic acid nanofibrous composite scaffold for novel wound dressing applications. International Journal of Biological Macromolecules 2023, 242 , 125093. https://doi.org/10.1016/j.ijbiomac.2023.125093
  48. Qiang Zeng, Zhigang Liu, Ting Niu, Chuan He, Ying Qu, Zhiyong Qian. Application of nanotechnology in CAR-T-cell immunotherapy. Chinese Chemical Letters 2023, 34 (3) , 107747. https://doi.org/10.1016/j.cclet.2022.107747
  49. Akihiro Moriyama, Isamu Ogura, Katsuhide Fujita. Potential issues specific to cytotoxicity tests of cellulose nanofibrils. Journal of Applied Toxicology 2023, 43 (1) , 195-207. https://doi.org/10.1002/jat.4390
  50. Mearg Gidey Berhe, Yemane Tadesse Gebreslassie. Biomedical Applications of Biosynthesized Nickel Oxide Nanoparticles. International Journal of Nanomedicine 2023, Volume 18 , 4229-4251. https://doi.org/10.2147/IJN.S410668
  51. Junjira Tanum, Moonhyun Choi, Hyejoong Jeong, Sohyeon Park, Chanutchamon Sutthiwanjampa, Hansoo Park, Jinkee Hong. Generation of zinc ion-rich surface via in situ growth of ZIF-8 particle: Microorganism immobilization onto fabric surface for prohibit hospital-acquired infection. Chemical Engineering Journal 2022, 446 , 137054. https://doi.org/10.1016/j.cej.2022.137054
  52. Hasan Saygin, Asli Baysal. Influence of Ca species on the surface properties of TiO2 nanoparticles and its possible transformation. Bulletin of Materials Science 2022, 45 (3)https://doi.org/10.1007/s12034-022-02735-z
  53. Mo’tasem M Alsmadi, Nusaiba K Al-Nemrawi, Rana Obaidat, Anwar E Abu Alkahsi, Khetam M Korshed, Ishraq K Lahlouh. Insights Into the Mapping of Green Synthesis Conditions for Zno Nanoparticles and their Toxicokinetics. Nanomedicine 2022, 17 (18) , 1281-1303. https://doi.org/10.2217/nnm-2022-0092
  54. Sixuan Piao, Donglan He. Sediment Bacteria and Phosphorus Fraction Response, Notably to Titanium Dioxide Nanoparticle Exposure. Microorganisms 2022, 10 (8) , 1643. https://doi.org/10.3390/microorganisms10081643
  55. Ayush Singha Roy, Aparna Sharma, Bhim Sen Thapa, Soumya Pandit, Dibyajit Lahiri, Moupriya Nag, Tanmay Sarkar, Siddhartha Pati, Rina Rani Ray, Mohammad Ali Shariati, Polrat Wilairatana, Mohammad S. Mubarak. Microbiomics for enhancing electron transfer in an electrochemical system. Frontiers in Microbiology 2022, 13 https://doi.org/10.3389/fmicb.2022.868220
  56. Marta Kędzierska, Nisrine Hammi, Joanna Kolodziejczyk-Czepas, Nadia Katir, Maria Bryszewska, Katarzyna Milowska, Abdelkrim El Kadib. Glassy-like Metal Oxide Particles Embedded on Micrometer Thicker Alginate Films as Promising Wound Healing Nanomaterials. International Journal of Molecular Sciences 2022, 23 (10) , 5585. https://doi.org/10.3390/ijms23105585
  57. Jayashree Shanmugam, Manikandan Dhayalan, Mohammed Riyaz Savaas Umar, Mayakkannan Gopal, Moonis Ali Khan, Jesus Simal-Gandara, Antonio Cid-Samamed. Green Synthesis of Silver Nanoparticles Using Allium cepa var. Aggregatum Natural Extract: Antibacterial and Cytotoxic Properties. Nanomaterials 2022, 12 (10) , 1725. https://doi.org/10.3390/nano12101725
  58. Murugeswaran Dayana Senthamarai, Balasubramanian Malaikozhundan. Synergistic action of zinc oxide nanoparticle using the unripe fruit extract of Aegle marmelos (L.) - Antibacterial, antibiofilm, radical scavenging and ecotoxicological effects. Materials Today Communications 2022, 30 , 103228. https://doi.org/10.1016/j.mtcomm.2022.103228
  59. Guoxin Cui, Wentao Su, Mingqian Tan. Formation and biological effects of protein corona for food‐related nanoparticles. Comprehensive Reviews in Food Science and Food Safety 2022, 21 (2) , 2002-2031. https://doi.org/10.1111/1541-4337.12838
  60. Rowland Burges, Surendar Varadharajan. A Short Review on Effects of Nano Metals on Human Health. 2022, 275-281. https://doi.org/10.1007/978-981-16-4400-9_20
  61. Anamika Singh, Dinesh K. Patel. Nanomaterials for Biomedical Engineering Applications. 2022, 75-102. https://doi.org/10.1007/978-981-19-1384-6_5
  62. Tsuyoshi Kawakami, Atsuko Miyajima, Kaoru Komoriya, Reiko Kato, Kazuo Isama. Effect of secondary particle size of nickel oxide nanoparticles on cytotoxicity in A549 cells. The Journal of Toxicological Sciences 2022, 47 (4) , 151-157. https://doi.org/10.2131/jts.47.151
  63. Jiali Yuan, Yue Zhang, Yuanbao Zhang, Yiqun Mo, Qunwei Zhang. Effects of metal nanoparticles on tight junction-associated proteins via HIF-1α/miR-29b/MMPs pathway in human epidermal keratinocytes. Particle and Fibre Toxicology 2021, 18 (1)https://doi.org/10.1186/s12989-021-00405-2
  64. Leonara Fayer, Rafaella S S Zanette, Juliana T C Siqueira, Eduarda R Oliveira, Camila G Almeida, Juliana C Gern, Saulo M Sousa, Luiz F C de Oliveira, Humberto M Brandão, Michele Munk. The distinct effect of titanium dioxide nanoparticles in primary and immortalized cell lines. Toxicology Research 2021, 10 (3) , 511-522. https://doi.org/10.1093/toxres/tfab040
  65. Masanori Horie, Yosuke Tabei. Role of oxidative stress in nanoparticles toxicity. Free Radical Research 2021, 55 (4) , 331-342. https://doi.org/10.1080/10715762.2020.1859108
  66. Joseph S. Erlichman, James C. Leiter. Complexity of the Nano-Bio Interface and the Tortuous Path of Metal Oxides in Biological Systems. Antioxidants 2021, 10 (4) , 547. https://doi.org/10.3390/antiox10040547
  67. Sharlee L. More, Michael Kovochich, Tara Lyons-Darden, Michael Taylor, Alexandra M. Schulte, Amy K. Madl. Review and Evaluation of the Potential Health Effects of Oxidic Nickel Nanoparticles. Nanomaterials 2021, 11 (3) , 642. https://doi.org/10.3390/nano11030642
  68. Dibyajit Lahiri, Moupriya Nag, Hassan I. Sheikh, Tanmay Sarkar, Hisham Atan Edinur, Siddhartha Pati, Rina Rani Ray. Microbiologically-Synthesized Nanoparticles and Their Role in Silencing the Biofilm Signaling Cascade. Frontiers in Microbiology 2021, 12 https://doi.org/10.3389/fmicb.2021.636588
  69. Zi-Jian Chen, Zhicheng Huang, Song Huang, Jin-Lin Zhao, Yuanming Sun, Zhen-Lin Xu, Juewen Liu. Effect of proteins on the oxidase-like activity of CeO 2 nanozymes for immunoassays. The Analyst 2021, 146 (3) , 864-873. https://doi.org/10.1039/D0AN01755H
  70. Mazhar Salim Al Zoubi, Alaa A. A. Aljabali, Kaushik Pal. Highly Toxic Nanomaterials for Cancer Treatment. 2021, 161-185. https://doi.org/10.1007/978-3-030-67223-2_8
  71. Rakesh Kumar Sahoo, Sarita Rani, Vinay Kumar, Umesh Gupta. Zinc oxide nanoparticles for bioimaging and drug delivery. 2021, 483-509. https://doi.org/10.1016/B978-0-12-818900-9.00021-8
  72. Anza-vhudziki Mboyi, Ilunga Kamika, Maggy N.B. Momba. Comparing the biosorption of ZnO and Ag nanomaterials by consortia of protozoan and bacterial species. 2021, 579-598. https://doi.org/10.1016/B978-0-12-823528-7.00013-5
  73. Pallavi Saxena, Vinod Saharan, Prabhat Kumar Baroliya, Vinod Singh Gour, Manoj Kumar Rai, Harish. Mechanism of nanotoxicity in Chlorella vulgaris exposed to zinc and iron oxide. Toxicology Reports 2021, 8 , 724-731. https://doi.org/10.1016/j.toxrep.2021.03.023
  74. Manikandan Dhayalan, Malathi Selvaraj, Kumar B Karthick, Riyaz S.U. Mohammed, Mika Sillanpää. Eco friendly synthesis and characterization of zinc oxide nanoparticles from Aegle marmelos and its cytotoxicity effects on MCF-7 cell lines. Nanofabrication 2021, 6 (1) , 44-51. https://doi.org/10.1515/nanofab-2020-0104
  75. Matthias P.L. Sentis, Giovanni Brambilla, Valérie Fessard, Gérard Meunier. Simultaneous screening of the stability and dosimetry of nanoparticles dispersions for in vitro toxicological studies with static multiple light scattering technique. Toxicology in Vitro 2020, 69 , 104972. https://doi.org/10.1016/j.tiv.2020.104972
  76. Federico G. Baudou, Luciano Fusco, Exequiel Giorgi, Eugenia Diaz, Sofía Municoy, Martín F. Desimone, Laura Leiva, Mauricio C. De Marzi. Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom. Colloids and Surfaces B: Biointerfaces 2020, 193 , 111128. https://doi.org/10.1016/j.colsurfb.2020.111128
  77. Jang Hyun Choi, Jung-A Hong, Ye Rim Son, Jian Wang, Hyun Sung Kim, Hansol Lee, Hangil Lee. Comparison of Enhanced Photocatalytic Degradation Efficiency and Toxicity Evaluations of CeO2 Nanoparticles Synthesized Through Double-Modulation. Nanomaterials 2020, 10 (8) , 1543. https://doi.org/10.3390/nano10081543
  78. Nadine Wiesmann, Wolfgang Tremel, Juergen Brieger. Zinc oxide nanoparticles for therapeutic purposes in cancer medicine. Journal of Materials Chemistry B 2020, 8 (23) , 4973-4989. https://doi.org/10.1039/D0TB00739K
  79. Linda Böhmert, Linn Voß, Valerie Stock, Albert Braeuning, Alfonso Lampen, Holger Sieg. Isolation methods for particle protein corona complexes from protein-rich matrices. Nanoscale Advances 2020, 2 (2) , 563-582. https://doi.org/10.1039/C9NA00537D
  80. Sekar Vijayakumar, Kandasamy Saravanakumar, Balasubramanian Malaikozhundan, Mani Divya, Baskaralingam Vaseeharan, Esteban F. Durán-Lara, Myeong-Hyeon Wang. Biopolymer K-carrageenan wrapped ZnO nanoparticles as drug delivery vehicles for anti MRSA therapy. International Journal of Biological Macromolecules 2020, 144 , 9-18. https://doi.org/10.1016/j.ijbiomac.2019.12.030
  81. A. Angel Ezhilarasi, J. Judith Vijaya, L. John Kennedy, K. Kaviyarasu. Green mediated NiO nano-rods using Phoenix dactylifera (Dates) extract for biomedical and environmental applications. Materials Chemistry and Physics 2020, 241 , 122419. https://doi.org/10.1016/j.matchemphys.2019.122419
  82. Ying Shi, Alexander R. Pilozzi, Xudong Huang. Exposure of CuO Nanoparticles Contributes to Cellular Apoptosis, Redox Stress, and Alzheimer’s Aβ Amyloidosis. International Journal of Environmental Research and Public Health 2020, 17 (3) , 1005. https://doi.org/10.3390/ijerph17031005
  83. Loutfy H. Madkour. Programmed cell death mechanisms and nanoparticle toxicity. 2020, 229-264. https://doi.org/10.1016/B978-0-12-822481-6.00010-4
  84. Jing-nan Feng, Xing-pan Guo, Yu-ru Chen, Da-pei Lu, Zuo-shun Niu, Fei-yun Tou, Li-jun Hou, Jiang Xu, Min Liu, Yi Yang. Time-dependent effects of ZnO nanoparticles on bacteria in an estuarine aquatic environment. Science of The Total Environment 2020, 698 , 134298. https://doi.org/10.1016/j.scitotenv.2019.134298
  85. Saeideh Nemati, Hasan Ali Hosseini, Alireza Hashemzadeh, Mohammad Mohajeri, Zahra Sabouri, Majid Darroudi, Reza Kazemi Oskuee. Cytotoxicity and photocatalytic applications of biosynthesized ZnO nanoparticles by Rheum turketanicum rhizome extract. Materials Research Express 2019, 6 (12) , 125016. https://doi.org/10.1088/2053-1591/ab46fb
  86. Neelu Singh, Monoj Kumar Das, Rohit Gautam, Anand Ramteke, Paulraj Rajamani. Assessment of intermittent exposure of zinc oxide nanoparticle (ZNP)–mediated toxicity and biochemical alterations in the splenocytes of male Wistar rat. Environmental Science and Pollution Research 2019, 26 (32) , 33642-33653. https://doi.org/10.1007/s11356-019-06225-4
  87. Robert A. Yokel, Matthew L. Hancock, Benjamin Cherian, Alexandra J. Brooks, Marsha L. Ensor, Hemendra J. Vekaria, Patrick G. Sullivan, Eric A. Grulke. Simulated biological fluid exposure changes nanoceria’s surface properties but not its biological response. European Journal of Pharmaceutics and Biopharmaceutics 2019, 144 , 252-265. https://doi.org/10.1016/j.ejpb.2019.09.023
  88. Safia Hameed, Javed Iqbal, Muhammad Ali, Ali Talha Khalil, Banzeer Ahsan Abbasi, Muhammad Numan, Zabta Khan Shinwari. Green synthesis of zinc nanoparticles through plant extracts: establishing a novel era in cancer theranostics. Materials Research Express 2019, 6 (10) , 102005. https://doi.org/10.1088/2053-1591/ab40df
  89. Pallavi Saxena, Harish. Toxicity assessment of ZnO nanoparticles to freshwater microalgae Coelastrella terrestris. Environmental Science and Pollution Research 2019, 26 (26) , 26991-27001. https://doi.org/10.1007/s11356-019-05844-1
  90. Greta Patrinoiu, Raluca Dumitru, Dana C. Culita, Cornel Munteanu, Ruxandra Birjega, José M. Calderon-Moreno, Andrei Cucos, Diana Pelinescu, Mariana C. Chifiriuc, Coralia Bleotu, Oana Carp. Self-assembled zinc oxide hierarchical structures with enhanced antibacterial properties from stacked chain-like zinc oxalate compounds. Journal of Colloid and Interface Science 2019, 552 , 258-270. https://doi.org/10.1016/j.jcis.2019.05.051
  91. L. A. Sharafutdinova, Yu. S. Zamula, Z. R. Khismatullina, M. R. Daminov, V. V. Valiullin. Structural and Biomechanical Characteristics of Blood Neutrophils on the Background of Exposure to Titanium Dioxide Nanoparticles (an atomic force microscopy study). Neuroscience and Behavioral Physiology 2019, 49 (6) , 791-796. https://doi.org/10.1007/s11055-019-00803-2
  92. P. L. Sanches, W. Souza, S. Gemini-Piperni, A. L. Rossi, S. Scapin, V. Midlej, Y. Sade, A. F. Paes Leme, M. Benchimol, L. A. Rocha, R. B. V. Carias, R. Borojevic, J. M. Granjeiro, A. R. Ribeiro. Rutile nano–bio-interactions mediate dissimilar intracellular destiny in human skin cells. Nanoscale Advances 2019, 1 (6) , 2216-2228. https://doi.org/10.1039/C9NA00078J
  93. Alyssa R. Deline, Jeffrey A. Nason. Evaluation of labeling methods used for investigating the environmental behavior and toxicity of metal oxide nanoparticles. Environmental Science: Nano 2019, 6 (4) , 1043-1066. https://doi.org/10.1039/C8EN01187G
  94. Jinhua Li, Meng Jiang, Huaijuan Zhou, Ping Jin, Kenneth M. C. Cheung, Paul K. Chu, Kelvin W. K. Yeung. Vanadium Dioxide Nanocoating Induces Tumor Cell Death through Mitochondrial Electron Transport Chain Interruption. Global Challenges 2019, 3 (3)https://doi.org/10.1002/gch2.201800058
  95. Byoung Chan Kim, Eunhoo Jeong, Eunju Kim, Seok Won Hong. Bio-organic–inorganic hybrid photocatalyst, TiO2 and glucose oxidase composite for enhancing antibacterial performance in aqueous environments. Applied Catalysis B: Environmental 2019, 242 , 194-201. https://doi.org/10.1016/j.apcatb.2018.09.102
  96. Abhishek Sharan, Seema Nara. Phytotoxic Properties of Zinc and Cobalt Oxide Nanoparticles in Algaes. 2019, 1-22. https://doi.org/10.1016/B978-0-12-811488-9.00001-9
  97. Ioan Valentin Tudose, Narcisa Vrinceanu, Cristina Pachiu, Stefan Bucur, Petronela Pascariu, Laurentiu Rusen, Emmanuel Koudoumas, Mirela Petruta Suchea. Nanostructured ZnO-based materials for biomedical and environmental applications. 2019, 285-305. https://doi.org/10.1016/B978-0-12-814401-5.00011-6
  98. Debora Barros Barbosa, Douglas Roberto Monteiro, Aline Satie Takamyia, Emerson Rodrigues de Camargo, Alessandra Marçal Agostinho Hunt, Alberto Carlos Botazzo Delbem, Juliano Pelim Pessan. Silver and phosphate nanoparticles: Antimicrobial approach and caries prevention application. 2019, 225-242. https://doi.org/10.1016/B978-0-12-815886-9.00009-7
  99. Yining Xia, Maria Rubino, Rafael Auras. Interaction of nanoclay-reinforced packaging nanocomposites with food simulants and compost environments. 2019, 275-298. https://doi.org/10.1016/bs.afnr.2019.02.001
  100. Sumreen Hayat, Saima Muzammil, Shabana, Bilal Aslam, Muhammad Hassnain Siddique, Muhammad Saqalein, Muhammad Atif Nisar. Quorum Quenching: Role of Nanoparticles as Signal Jammers in Gram-Negative Bacteria. Future Microbiology 2019, 14 (1) , 61-72. https://doi.org/10.2217/fmb-2018-0257

Load more citations

Chemical Research in Toxicology

Cite this: Chem. Res. Toxicol. 2009, 22, 3

Click to copy citationCitation copied!

Published February 16, 2009

Copyright © 2009 American Chemical Society

Altmetric

-

Citations

Learn about these metrics

Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.