A modified Boyden chamber assay for tumor cell transendothelial migration in vitro (original) (raw)

Abstract

We have developed a modified Boyden chamber system for assessing tumor cell transmigration across endothelial monolayer in vitro. This radiometric-based method allows us to take into account the cells which have traversed but detached from the filter, a significant fraction not included in the existing method of visual counting. The method was applied to examine six malignant and one non-malignant cell lines. The variable invasive potentials of these cells were found to correlate with their ability to disrupt the endothelial cell monolayers.

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Authors and Affiliations

  1. George W. Woodruff School of Mechanical Engineering and Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, 30332-0405, USA
    Yu-Hua Li & Cheng Zhu

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  1. Yu-Hua Li
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  2. Cheng Zhu
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Li, YH., Zhu, C. A modified Boyden chamber assay for tumor cell transendothelial migration in vitro.Clin Exp Metastasis 17, 423–429 (1999). https://doi.org/10.1023/A:1006614232388

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